| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,689,824 | T→G | 50.2% | R238R (CGT→CGG) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,689,824 | 0 | T | G | 50.2% | 8.0 / 24.8 | 35 | R238R (CGT→CGG) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (9/8); new base G (3/15); total (12/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01 | |||||||||||
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG > KT2440/5689692‑5689958 | aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACGTCAGCCACCTAACCAGCGCCCCTgg > 2:261917/1‑135 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGGCCGTCAGCCAGCTAACCACCGCCCGGGGcgcgcg > 7:217826/1‑136 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACATCAGCCACATCACCACCGCCCCTGGcgcgcg > 8:73297/1‑136 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGGCCCTTACGCCAGATCACCAGCCCCCGTGgcgcgc > 6:171795/1‑135 (MQ=255) tGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCTCCCGTGGCGCGCGGc < 5:333042/135‑1 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCCGCCCGCTCCCCAGCCCCCGGGGCGCGCCGCTGCTc > 2:121087/1‑135 (MQ=255) aGCGTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACGGTGGCCGTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCg < 7:326956/136‑1 (MQ=255) tgggCGTGCCGGTCATCCCGGAGAGCGCCTAAACCGTGGCCCTGG‑CCCGAACCCTGCTGCGGGGGGACCTGAGCGGCGGGCGTGCGAACTTCAGCCAGACCACCCGCGCCGGTGGCGCGCGGCTGATCgcccagg < 6:325774/133‑1 (MQ=255) cggccggcGGGGCATCCCGTAGAGCCCCGAAACCGTGGCCCTGG‑CGCGAAACCTGGTGCTGGTGTACCTGTGGGGGGTGCGTGCGAACTGCTCCCACATCACCCGCGCCGGGGGCGCGCGGCTGATCgcccaggc < 6:429458/135‑1 (MQ=255) cTGCCGGGCATCCGGGAGCGCGCCTAACCCGGGCCCGTGG‑CGCGCAACCTGCGGGTGGGGGAACAGCGCGGCGTGCGGGCCCACTCCAGCCAGATCACCCGGGCCGGGGGCGCGCGGCTGATCgcccaggcccag < 2:33762/135‑1 (MQ=255) gCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCCATTCCCCCCGATCACCACCGCCCGGGGCGCGCGGCTGTTAGCCcccgcccacgcc > 8:19019/1‑136 (MQ=255) gcgcCGAACCCGTGGCCCGGG‑CGCGCAACAGGCGGCGGGGGGAACAGCGCGGCGTGCGGGCGCACTGCAGCCACATCACCCGGGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 7:342818/136‑1 (MQ=255) cgcCGAAACCGGGGCCAGGG‑GGCGCAACCTGCTGCGGGGGTAACACAGCGGCTGGGGGGCGAACTTAACCCTAATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 6:414281/135‑1 (MQ=255) ggTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 7:236673/135‑1 (MQ=255) gTGGCCCTGG‑CGCGCAACCTGCTGCTGGGGTACCAGCGGGGCGTGCGGGCGCACTGCCGCCAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:201994/135‑1 (MQ=255) gggCCCCGGCCGCGCACCGGGCGGCGGGGGGACCCGAGGGGGGGGCGTGCGCACTTCCGCCAGACCCCCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:328696/134‑1 (MQ=255) gcgcACCCTGGGGCTGGGGGAACAGCGCGGGGGGGGGGCGCACTTCCCCCATACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg < 7:61725/136‑1 (MQ=255) cgcACCCTGCGGGGGGGGGAACAGAGGGGCGGGCGTGCGCACTTCAGCCAGAGCCCCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg < 4:57641/135‑1 (MQ=255) gAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCACCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCCGCCTTGGGCCTTCCGGTAACCGCCGCTGTAGCGCTGTGACAGCTGATCCTCCCGGCCGAGt > 7:288959/1‑136 (MQ=255) cggggggcgggcgCCCTTCCGCCCGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 2:378721/129‑1 (MQ=255) cggcggGCGGGCGCACTCCCCCCAGACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 4:14850/135‑1 (MQ=255) cggcgTGCGGGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 4:267889/135‑1 (MQ=255) ggcgTGGGTGCCCACTTCCGCCAGCTCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc < 4:161674/135‑1 (MQ=255) gggggcgcACTGCCCCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt < 6:437551/131‑1 (MQ=255) gcgtgcgCACTTCAGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCATGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt < 2:62820/135‑1 (MQ=255) gcgggcgCACCACAGCCATACCCCCCGCGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc < 8:205594/136‑1 (MQ=255) gcgcAGTGCAGCCAGAGCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 6:200718/135‑1 (MQ=255) ccccAGATCCCCCGCGCCCGGGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGt < 6:82152/131‑1 (MQ=255) ccAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTGCTCCAGCCTGTACCa > 5:254788/1‑135 (MQ=255) ccccgggcCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg < 2:246378/129‑1 (MQ=255) cgcgcCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgccg < 5:171795/134‑1 (MQ=255) gcCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGccgccg > 5:202499/1‑135 (MQ=255) cccGTGGCGCGCGGCTAATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCcgccgc > 3:243752/1‑135 (MQ=255) gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACGAGCTGATCCTCACGGACGAGTCGCTGCGCGCGGTCTCCAGCCTGTACCACGTGCCGCCGCCGCTgcg > 8:359969/1‑136 (MQ=255) gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGCGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgc > 2:180207/1‑135 (MQ=255) | AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGG‑CGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG > KT2440/5689692‑5689958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |