Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	336,468	T→G	25.9%	K9N (AAA→AAC)	CJ019_00309 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	336,468	0	T	G	25.9%	99.9 / 5.0	61	K9N (AAA→AAC)	CJ019_00309	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/32);  new base G (16/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 7.48e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGAT  >  KT2440/336336‑336598                                                                                                                                     |                                                                                                                                    gtAGCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGttt                                                                                                                                   <  2:130180/135‑1 (MQ=255)     cTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTg                                                                                                                                <  5:140893/134‑1 (MQ=255)          cGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCtt                                                                                                                         <  3:78265/135‑1 (MQ=255)          cGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCtt                                                                                                                         <  3:236736/135‑1 (MQ=255)          cGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCtt                                                                                                                         <  3:193088/135‑1 (MQ=255)           gACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGGGCGGTTATT‑TTGCTTg                                                                                                                        >  5:148690/1‑135 (MQ=255)                     ccTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTg                                                                                                               <  1:371935/134‑1 (MQ=255)                         gttgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATg                                                                                                         <  8:169040/136‑1 (MQ=255)                           tgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGGGTGGCGCGGGTCTG‑TTGGTTGGTATGGGTGGGCATgg                                                                                                        >  2:371935/1‑135 (MQ=255)                           tgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATg                                                                                                         <  1:12023/134‑1 (MQ=255)                             tACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCGTGGTAGGGGTGGCCATgggg                                                                                                      >  8:218645/1‑135 (MQ=255)                             tACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCGTGGTAGGGGTGGCCATggg                                                                                                       >  3:411675/1‑134 (MQ=255)                               cacCTGCTCGACGCGGGCGCTGTCGCCGGCTCTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGaaa                                                                                                   <  7:387200/136‑1 (MQ=255)                                acCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGACTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGaa                                                                                                    <  6:59882/134‑1 (MQ=255)                                  cTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGGGGTTGAG‑GTGGGTTGTAGGGGGGGCCAGGGGGTAAtg                                                                                                 >  1:120301/1‑134 (MQ=255)                                  cTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGTAATcc                                                                                                >  8:335688/1‑136 (MQ=255)                                  cTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTGTG‑TTGGGTGGGAGGGGGGGGCCTGGGGTAAtg                                                                                                 >  5:73168/1‑134 (MQ=255)                                     cTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGGGCTTGGGGCGTTTCTG‑TTGGTTGGGTTGGGTGGCCCTGGGGAAATcggt                                                                                              >  6:46853/1‑132 (MQ=255)                                      tCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTg                                                                                             <  5:89497/135‑1 (MQ=255)                                      tCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTg                                                                                             <  3:330129/135‑1 (MQ=255)                                      tCGACGCTGGCGCTGGCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCGTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTg                                                                                             <  4:25369/135‑1 (MQ=255)                                      gcGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTg                                                                                             <  4:388941/134‑1 (MQ=255)                                       cGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTGTG‑TTGCGTGGTAGGGGTGGCCCTGGGGAAATGCTTgg                                                                                            >  4:160992/1‑135 (MQ=255)                                       cGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTGGG‑TTTGTTGGTAGGGGGGGGCATGGGGGAAtaggtgg                                                                                            >  3:423755/1‑129 (MQ=255)                                        gACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCCCGCTTGGCGCGTTTGGG‑TTGGTTGGTTGGGGTGGGCAAGGGGTAATACTTggg                                                                                           >  6:416955/1‑135 (MQ=255)                                                  cTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTCGG‑GTGCTTGGTAGGGGTGGGCATGGGGAAATCCGTGGGGGAGtgtg                                                                                   >  6:91124/1‑133 (MQ=255)                                                  cTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCAGGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTGGGGGCGGTTGGG‑TTGGGTGGGAGGGGGGGGCCTGGGGGAATCCGGGGGTGGGTCTg                                                                                   >  6:418049/1‑133 (MQ=255)                                                                        ccGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCaa                                                            <  4:302459/134‑1 (MQ=255)                                                                         cGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTCTG‑TTGCTTTGGAGGGGGGGCCCTGGGGTAATCAGTTGGGGAGTGTGAAAGTGCGCGTTTTGAAGCGCAAtt                                                          >  6:124815/1‑135 (MQ=255)                                                                              ggCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTGTG‑TTGGTTGGTTGGGGTGGCCCTGGGGAAATCCTTGGGTGAGTGTGAAAGTGCGCGTATTGAAGCGCAATTGCCCg                                                     >  1:78245/1‑135 (MQ=255)                                                                              ggCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGGTATG‑TTGGTTGGTAGGGGTGGCCATGGGGAAAACCGTGGGTGAGTCTGTAAATTCGCGGATTGAAGCGCAAATGgcc                                                      >  2:372893/1‑134 (MQ=255)                                                                              ggCCGCTGCGTACCATGAGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCGTGGTAGGGGTGGCCATTGGGAAATCCTTGGGTGGGTCTGAAATTGCCCGTATTGAAGCGCAATTGccc                                                      >  7:373685/1‑134 (MQ=255)                                                                               gCCGCTGCTTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGGGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCg                                                     <  4:307866/134‑1 (MQ=255)                                                                                cggCTGCGTTCCATGCGGTTCTGGTTGGCCTTGTTGGGGGGGCGGTGGGGGCGTTTCTG‑TTGCTTGGTTGGGGTGGCCATGGGGAATTGCTTGGGTGTGTCTGAAAGTGCGCGTTTTGAAGCGCAATTGCCCGAt                                                   <  5:363514/133‑1 (MQ=255)                                                                                    tGCGTACCATGCGGTTCTGCTTGGCCTGGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAAc                                               <  3:160992/135‑1 (MQ=255)                                                                                         gccATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTGGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCAGGGGTAAATCATTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAg                                          <  1:328566/134‑1 (MQ=255)                                                                                         aCCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAg                                          <  5:379158/135‑1 (MQ=255)                                                                                         aCCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTG‑TTGCTTGGTCGGGGGGGCCATGGGTAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAg                                          <  2:183022/135‑1 (MQ=255)                                                                                                gggtCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGGAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGgcgcg                                   <  6:211996/132‑1 (MQ=255)                                                                                                ggTTCTGCTTGGCCTTGCTGGCGGCGCGGTTGGGGGGGTTGTGTTTGGTGGGTGGGGGGGGCC‑TGGGGGAATGCGTGGGGGTGTGGGAAATTGGGGGTTTTTTAGCGCCATTTCCCCCTAAACCCCACCcgcgcg                                   >  6:379158/1‑135 (MQ=255)                                                                                                ggTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTG‑TTGGTTGGGTGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAATTGCGCGTATTGAAGCGCAAATGCCCGATAAACCCAAGCGgcgcg                                   >  4:366788/1‑135 (MQ=255)                                                                                                 gTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGcc                                 >  8:241163/1‑136 (MQ=255)                                                                                                 gTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATGCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGcc                                 >  8:15382/1‑136 (MQ=255)                                                                                                       cTTGGCCTGGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgcg                           <  7:402378/136‑1 (MQ=255)                                                                                                        ttGGCCTTGCTGGCGGCGCGCTTGTCGCGTTTCTT‑TTTCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGACTCTGAAAGTTCGCGTATTTAAGCGCAATTGCCAGATAAACCCAAACGGCGCGCCTGGgcgc                          >  8:342282/1‑136 (MQ=255)                                                                                                        ttGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTG‑TTGCGTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTGTGAAATTGGGCGTATTTAAGCGCAATTGCCCGATAAACCCCAGCCGCGCGCCGGGgcc                           >  2:328566/1‑134 (MQ=255)                                                                                                        ttGGCCTTGCTGGCGGCGCGCTGGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgc                            <  5:91124/134‑1 (MQ=255)                                                                                                        ttGGCCTTGCTGGCGGCGCGCGTTGCGCGGTTGTT‑TTGGGTGGTAGGGGTGGCCATGGGGTAAACCTTGGGTGTGTGAGAAATTTCCCGGATTTAATCGCCATTTGCCGATAAACCCAAACCGCGCCCCGGGgcg                           >  4:387775/1‑135 (MQ=255)                                                                                                           gCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgcgc                          <  4:411675/133‑1 (MQ=255)                                                                                                            ccTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCa                      <  7:241163/136‑1 (MQ=255)                                                                                                               tGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCt                   >  7:149365/1‑136 (MQ=255)                                                                                                               tGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCt                   >  8:317273/1‑136 (MQ=255)                                                                                                                gcgggcggcGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCt                   <  1:156035/132‑1 (MQ=255)                                                                                                                 cTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGGGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCtt                  <  1:348927/135‑1 (MQ=255)                                                                                                                   ggcggcGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTga                <  2:78245/135‑1 (MQ=255)                                                                                                                    gcggcgCGCTTGGCGCGGTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCtt                  >  8:238202/1‑132 (MQ=255)                                                                                                                       gcgcggTTGGGGCGTTTCTG‑TTGCTTGGTCGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTTAAAGTGCGCGTATTCAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATg            <  1:137230/135‑1 (MQ=255)                                                                                                                       gcgcgcTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATg            <  5:170979/135‑1 (MQ=255)                                                                                                                       gcgcgcTGGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATg            <  5:389143/135‑1 (MQ=255)                                                                                                                          cgcTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCtg        <  7:347669/136‑1 (MQ=255)                                                                                                                                gcgcGTTTCTG‑TTGCTGGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGGCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGa   <  6:73168/135‑1 (MQ=255)                                                                                                                                gcgcGGTTCTG‑TTGCGTGGTAGGGGTGGCCATGGTGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGAt  <  8:373685/136‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    GTAGCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTG‑TTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGAT  >  KT2440/336336‑336598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑