Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,859,001 T→G 22.0% T329P (ACC→CCC)  cadA_2 ← Cadmium‑transporting ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,859,0010TG22.0% 92.9 / 3.6 48T329P (ACC→CCC) cadA_2Cadmium‑transporting ATPase
Reads supporting (aligned to +/- strand):  ref base T (8/28);  new base G (10/2);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 2.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAACACCACCGGGGTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACTTCA  >  KT2440/5858874‑5859134
                                                                                                                               |                                                                                                                                     
gCGAACACCACCGGGGTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGGGCcgcgc                                                                                                                                >  8:356993/1‑134 (MQ=255)
 cGAACACCACCGGGGTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCTCCAGGGTCGACGTCCCGGCAGCGGCGGTGGcgggg                                                                                                                                >  2:162857/1‑131 (MQ=255)
 cGAACACCACCGGGGTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGGGGGGAcccgcc                                                                                                                               >  4:112754/1‑133 (MQ=255)
  gAACACCACCGGGGTGTAGATGCGCGAAAACCGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGGGGGGCcgcgcca                                                                                                                              >  5:150903/1‑132 (MQ=255)
  gAACACAACCGGGGTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCCGCCGGGACGCGaa                                                                                                                               >  4:322348/1‑134 (MQ=255)
          ccGGGGTGTAGATGCGCTAAAAACGGTCGACAACGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAgcg                                                                                                                      <  6:193456/135‑1 (MQ=255)
             gggTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGCCGCGAAACTCCAGCGccc                                                                                                                   >  4:163054/1‑135 (MQ=255)
             gggTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCCGTGCCGCGAAACTCCCGCGccc                                                                                                                   >  2:294149/1‑135 (MQ=255)
              ggTGTAGATGCGCGAAAACCGGTCGACAAAGCGCGGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGcccc                                                                                                                  <  2:32665/135‑1 (MQ=255)
                     aTGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGt                                                                                                          <  8:359306/136‑1 (MQ=255)
                     aTGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGGCGCCCCAAACACCACCGCCCCCCCCAgg                                                                                                           >  4:157228/1‑135 (MQ=255)
                      tGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGt                                                                                                          <  4:419142/135‑1 (MQ=255)
                      tGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGt                                                                                                          <  2:12608/135‑1 (MQ=255)
                             aaaaCGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGGGGGGGCGCGCAACCACCCCGCCCCCCCCCGGGTggttgt                                                                                                   >  3:408659/1‑130 (MQ=255)
                             aaaaCGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGCCGCGCAACTCCAGCGCCCCCGCCGGGTTGATGGt                                                                                                   >  4:45174/1‑135 (MQ=255)
                             aaaaCGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGCCGCGCAACGCCCGCGCCCCCGCCGGGTTGCTGGt                                                                                                   >  4:58293/1‑135 (MQ=255)
                             aaaaCGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGGGCCGCGGCACGCCCGCCCCCCCCCCGGGGtggtgggg                                                                                                  >  8:446403/1‑134 (MQ=255)
                             aaaaCGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGGGACGCGCAACTCCCGCGCCCCCGCCGGGGTggaggt                                                                                                   >  5:343142/1‑130 (MQ=255)
                             aaaaCGGTCGACAAAGCGCTGCGAAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCCGTGAAGCGAAACTACCGCCCCCCCGCCGTGTTGATGGt                                                                                                   >  6:427533/1‑135 (MQ=255)
                                   gTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGc                                                                                             <  6:450614/135‑1 (MQ=255)
                                   gTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGc                                                                                             <  2:4626/135‑1 (MQ=255)
                                               cTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTca                                                                                <  7:296316/136‑1 (MQ=255)
                                                 gggTTGGCGCCCGCGCGCCTGGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGGGCCAGCGAACAGCTTGTcac                                                                               <  6:280934/135‑1 (MQ=255)
                                                  ggTAGGCGCCCGCGCGCCTTGCGCGTCCTCCACCGCCTTGATCATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTcaccc                                                                             <  7:356993/136‑1 (MQ=255)
                                                   gTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTcaccc                                                                             <  1:294149/135‑1 (MQ=255)
                                                          cccGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCTGCGCCAGGGTCGACTGCCCGGCAGCGGCGGGGGCGGGGCACACCCGCCCCCCCCCCCGGGTGGTGGTGGCCGCGCACAGGGTGTGACccccccccg                                                                      >  4:39959/1‑134 (MQ=255)
                                                                 gcCTTGCGCTTCCTCCACCGCCTTGATGCTCCGCGCCAGGGGCGACTGCCCGGCAGCGGCGGGGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCcac                                                               <  2:248956/135‑1 (MQ=255)
                                                                 gcCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCcac                                                               <  3:322348/135‑1 (MQ=255)
                                                                 gcCTTGCGCTTCATCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCcac                                                               <  1:141904/135‑1 (MQ=255)
                                                                 gcCTGGCGCTGCCTCCCCCGCCTGGAGGAGCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCcac                                                               <  5:23644/135‑1 (MQ=255)
                                                                     tGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCACCGCCCCCCCCAGGTTGATGGTGCCAGCGCACACCTTGTCAACCCCCCCCAAATCCAcacgca                                                          >  7:314329/1‑136 (MQ=255)
                                                                         cTTCCTCCACCGCGTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACaggcaggc                                                       <  2:257514/135‑1 (MQ=255)
                                                                                cACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGGGGCGCGCAACTCCACCCCCCCCCCCCGGGTTCTGGTTGCCGCGGACAGCGTGGGGCCCCCCCCCATCGCCACCGCCAAGCTTTGACCg                                               >  7:76696/1‑136 (MQ=255)
                                                                                              tCCGGGCCAGGGTCTACTGCCCTGAAGCTGCGGTGACGCGAAACTCCAGCCCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCt                                  <  1:162857/135‑1 (MQ=255)
                                                                                              tCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCt                                  <  3:45174/135‑1 (MQ=255)
                                                                                                 gcgcCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGa                                <  4:204724/134‑1 (MQ=255)
                                                                                                   gcCAGGGTCGTCGGGCCGGCAGCGGGGGGGACGCGAAACTCCACCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCg                             <  3:163054/135‑1 (MQ=255)
                                                                                                   gcCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCGCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCg                             <  3:112754/135‑1 (MQ=255)
                                                                                                        ggTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGGGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCt                        <  5:157700/135‑1 (MQ=255)
                                                                                                                gCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCGGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGccc               <  8:24070/136‑1 (MQ=255)
                                                                                                                     gacgcggcgGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCggg          <  8:345560/133‑1 (MQ=255)
                                                                                                                      cAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCggg          <  4:394349/135‑1 (MQ=255)
                                                                                                                          ggcggTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACGCCCTTCTCCTCAGGCAGTCTTTAACCTGTAATCGGCGCCTGATCGAAGCTGGATTGCCCGCGGGTCACt     <  8:181421/136‑1 (MQ=255)
                                                                                                                          ggcggTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACt     <  8:353500/136‑1 (MQ=255)
                                                                                                                           gcggTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACt     <  4:408659/135‑1 (MQ=255)
                                                                                                                           gcggTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACt     <  2:403439/135‑1 (MQ=255)
                                                                                                                           gcggTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACt     <  5:168519/135‑1 (MQ=255)
                                                                                                                            cggTGACGCGAAACTCCAGCG‑CCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACTTCa  >  8:371442/1‑136 (MQ=255)
                                                                                                                               |                                                                                                                                     
GCGAACACCACCGGGGTGTAGATGCGCGAAAAACGGTCGACAAAGCGCTGGGTAGGCGCCCGCGCGCCTTGCGCTTCCTCCACCGCCTTGATGATCCGCGCCAGGGTCGACTGCCCGGCAGCGGCGGTGACGCGAAACTCCAGCGCCCCCGCCTGGTTGATGGTGCCAGCGAACAGCTTGTCACCCACCCCCTTCTCCACAGGCAGGCTTTCACCGGTAATCGGCGCCTGATCGACGCTGGATTGCCCGCGGGTCACTTCA  >  KT2440/5858874‑5859134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑