Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,890,917	C→T	6.6%	A96T (GCC→ACC)	sbp_2 ←	Sulfate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,890,917	0	C	T	6.6%	200.4 / 2.3	60	A96T (GCC→ACC)	sbp_2	Sulfate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base T (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCAGGTCGCCCCAGTCCTTGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCACCCGGGTGGGCCTGTT  >  KT2440/5890783‑5891051                                                                                                                                       |                                                                                                                                       gATCAGGTCGCCCCAGTCCTTGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGc                                                                                                                                        >  4:317177/1‑135 (MQ=255)          gCCCCAGTCCTTGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCACGGTCTTGCCAAGCTTGGCCACTtcgtc                                                                                                                               >  7:223457/1‑135 (MQ=255)              cAGTCCTTGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATg                                                                                                                           <  6:227635/135‑1 (MQ=255)                  ccTTGATGCCTTTCGGGGTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCAcc                                                                                                                      <  7:358900/136‑1 (MQ=255)                  ccTTGATGCCTTTCGGGGTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCAcc                                                                                                                      <  7:56605/136‑1 (MQ=255)                   cTTGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCAcc                                                                                                                      <  7:77688/135‑1 (MQ=255)                    ttGATGGCTTTCGGGTTGCGCTTGCGCACCAGGAACAAGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCTGGCAGGGTCTTGCGAAGCTTGTCCACTTCGTCGATGTCAcc                                                                                                                      <  6:203036/134‑1 (MQ=255)                    ttGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGGCAcc                                                                                                                      <  1:243435/134‑1 (MQ=255)                    ttGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGGACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCAcc                                                                                                                      <  4:251638/134‑1 (MQ=255)                        tGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGACGATGTCACCcgccag                                                                                                                >  8:83392/1‑136 (MQ=255)                         gCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGTCACTTCGTCGATGTCACCggccagg                                                                                                               >  7:359206/1‑136 (MQ=255)                         gCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGTCACTTCGTCGATGTCACCggccagg                                                                                                               >  7:352818/1‑136 (MQ=255)                              tCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCggccagggcc                                                                                                            <  7:391816/134‑1 (MQ=255)                                gggTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGgccagggccagg                                                                                                         >  1:347398/1‑135 (MQ=255)                                 ggTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGt                                                                                                       <  7:74155/136‑1 (MQ=255)                                 ggTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTGCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGccagggccaggg                                                                                                        <  2:210441/135‑1 (MQ=255)                                        cTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTcaccac                                                                                                 >  6:137653/1‑135 (MQ=255)                                              cACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGGCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGgc                                                                                           <  4:203348/135‑1 (MQ=255)                                               aCCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGgcgc                                                                                         >  7:196068/1‑136 (MQ=255)                                               aCCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGgcg                                                                                          >  1:242880/1‑135 (MQ=255)                                               aCCAGGAACACGATGGTCGAGGTGTACGGGGGGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGgcgc                                                                                         <  8:55597/136‑1 (MQ=255)                                                    gAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAg                                                                                     >  3:378293/1‑135 (MQ=255)                                                      acacGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGgcgcgcccgc                                                                                   >  1:230922/1‑131 (MQ=255)                                                          gATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTc                                                                               <  2:12659/135‑1 (MQ=255)                                                             ggTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGAt                                                                            >  6:74867/1‑135 (MQ=255)                                                              gTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGGCGCTc                                                                           >  1:376579/1‑135 (MQ=255)                                                                     tgtACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGCTCACCGCAc                                                                    >  5:386811/1‑135 (MQ=255)                                                                       tACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACggg                                                                 >  8:149761/1‑136 (MQ=255)                                                                                 tGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGc                                                        >  1:30181/1‑135 (MQ=255)                                                                                 gggCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGcc                                                       <  7:12470/135‑1 (MQ=255)                                                                                  ggCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCTGTCGATCACCGCACGGGCCTGCTTGcc                                                       >  2:310941/1‑135 (MQ=255)                                                                                  ggCGTCAGGCAGGAGGGTCTGCCACTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGCGCTGcaagc                                                        >  3:307420/1‑130 (MQ=255)                                                                                   gCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGccc                                                      <  6:6758/135‑1 (MQ=255)                                                                                   gCGTCAGGCAGGCGGGGCTGCCAGTGCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGccc                                                      <  5:211809/135‑1 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCATGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCCAACCGGCCAGCATGCCCGa                                                    >  8:182365/1‑136 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCg                                                     <  6:417853/135‑1 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCg                                                     <  1:204456/135‑1 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGa                                                    >  8:442466/1‑136 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGa                                                    >  8:352517/1‑136 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGGCGATCACCGCACGGGCCTGCTTGCCCgc                                                    >  7:330428/1‑135 (MQ=255)                                                                                    cGTCAGGCAGGCGGGTCTGCCAGTGCTCGGGCAGGGTCGTGCCAAGCTTGGCCACTTCGTCGATGGCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCGTGcc                                                       <  2:215745/133‑1 (MQ=255)                                                                                         ggcaggcGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCCGGCCGTCGCTCCCCGCACGGGCCTGCGTGCCCGAcccc                                                >  6:337347/1‑135 (MQ=255)                                                                                          gctggcGGGTCTGCCAGTGCTCGGGCTGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGAccccc                                               <  1:230261/132‑1 (MQ=255)                                                                                           caggcGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCGACTTCGTCGATGTCTCCGGCCAGGGCCAGGGTCACCACGTCGGCGCCCACCGCGACGATCACCCCACCGGCCAGCTTGCCCGAcccccc                                              >  7:275/1‑135 (MQ=255)                                                                                           caggcGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCCGGCCGTCGATCCCCGCACCGGCCTGCGTGCCCGAccccc                                               >  2:179020/1‑134 (MQ=255)                                                                                           caggcGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCCGGCCGTCGATCACCGCCCCGGCCTGCTTGCCCGAcccccc                                              >  8:12470/1‑135 (MQ=255)                                                                                           caggcGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGCGCCTGCTGGCCCGAccccccc                                             >  8:324363/1‑135 (MQ=255)                                                                                                gggTCTGCCAGTTCTCGGGCCGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGGCGCTCACCGCCCGGGCCGGCAGGCCCGAcccccccgggg                                         >  1:321780/1‑130 (MQ=255)                                                                                                   tCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGAtt                                      <  3:206508/135‑1 (MQ=255)                                                                                                                gggCAGGGTCTTGCCAAGCTTGGTCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCAc                         <  8:352818/135‑1 (MQ=255)                                                                                                                gggCAGGGTCTTGCCAAGCTTGGTCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCAc                         <  8:359206/135‑1 (MQ=255)                                                                                                                         cTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCTCCTTGTCAccc               <  8:166125/136‑1 (MQ=255)                                                                                                                         cTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCAccc               <  7:215588/136‑1 (MQ=255)                                                                                                                         cTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGCGCCTGCTTGCCCGACCCCCCATGGGATTGCAGGAATTTCACCATGTCaac                >  3:191896/1‑133 (MQ=255)                                                                                                                           tGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCACCCgg             <  7:214905/136‑1 (MQ=255)                                                                                                                              cAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCTGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCACCCGGGt           <  1:310941/135‑1 (MQ=255)                                                                                                                               aaGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGCTCACCGCACGGGCCGGCTTGCCCGCCCCCCCCGGGGATTGGTGGATTTTCCCCGTGTCACCCggggg          >  3:10666/1‑133 (MQ=255)                                                                                                                                   ttGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGcc                                                       <  7:129169/86‑1 (MQ=255)                                                                                                                                   ttGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGcc                                                       >  8:129169/1‑86 (MQ=255)                                                                                                                                   ttGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGCCCCCCCATGGGATTGCAGGATTTTCACCTTGTCACCCGGGAGGGCCt     >  8:365991/1‑136 (MQ=255)                                                                                                                                   ttGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCACCCGGGTGGGcc      >  6:182350/1‑135 (MQ=255)                                                                                                                                       ccACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCACCCGGGTGGGCCTGtt  <  2:347398/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GATCAGGTCGCCCCAGTCCTTGATGCCTTTCGGGTTGCCCTTGCGCACCAGGAACACGATGGTCGAGGTGTACGGGGTGCTGGCGTCAGGCAGGCGGGTCTGCCAGTTCTCGGGCAGGGTCTTGCCAAGCTTGGCCACTTCGTCGATGTCACCGGCCAGGGCCAGGGTCACCACGTCGGCGCGCAGGCCGTCGATCACCGCACGGGCCTGCTTGCCCGACCCCCCATGGGATTGCTGGATTTTCACCTTGTCACCCGGGTGGGCCTGTT  >  KT2440/5890783‑5891051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑