| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 6,016,987 | A→C | 52.5% | intergenic (+67/+77) | puuB_8 → / ← CJ019_05632 | Gamma‑glutamylputrescine oxidoreductase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,016,987 | 0 | A | C | 52.5% | 0.5 / 10.1 | 22 | intergenic (+67/+77) | puuB_8/CJ019_05632 | Gamma‑glutamylputrescine oxidoreductase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/6); new base C (3/8); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01 | |||||||||||
CAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCA > KT2440/6016854‑6017111 | cacgCACCGGCGCTCGCCACTGCTGGCGCTGGGAATGCTGGGGCATCGCCGCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCACCGGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGc < 8:425261/133‑1 (MQ=255)cAAGCACCTGCGCTCGCCACTGCTGGCGCTTGGAATGCGGGGGCATCGCCTCAAAGACCTCACTTGACAGACCCCATCCATGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGc < 8:441016/136‑1 (MQ=255) aCTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGGGCGGGCGTGGCCGcggccc > 1:52298/1‑130 (MQ=255) aCTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGCGGGGGGGGGCCCGCGccccc > 7:267417/1‑134 (MQ=255) tgctgGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGAGTGCCCGCGCCcacc > 6:134711/1‑134 (MQ=255) gggAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCATCTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGGCCCCGCCCCCAGGGTGGGGGc > 8:361115/1‑135 (MQ=255) cgggggCAGCGCCTCAAAGCGCGGACTTGAACGTCCCCATCCTTGGGGCACCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCGGGGGGAGCGGGCGGGCCCGCGCCCACAGGGTCGGTGCAAGTTTCa < 8:336189/132‑1 (MQ=255) gccagcGCCTAAAACCGCTGACTGTAACGGCCCCAGCCTGGGGCCACCAACTGGCTTGCTAAAAATCAAACCGCTCCCGGCCCCCCTGGGGGCGGGGGCGTGCCGGCACCCACAGGGTCGGTGCAAGTTTCAAGt < 4:30734/130‑1 (MQ=255) aCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGCGCGGGCGTGCCCGCGCCCCCCCGGGCGGGGCAACTTTGAAGTCGGGGGCACAACCCGcccgc > 1:89503/1‑133 (MQ=255) aCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCCGGCGTTCCCCCTCCCACACGGTCGGTGCAAGTTTGAAGTAAGGGGCACAACACGccccg > 3:200596/1‑133 (MQ=255) cTTGTGGCTGCAAGTGGCTTGGTTAAAATCTAAACGCTCACGGGCCCCTTGTGGGCGCGGGCTGGCCGGGGCCCAGAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAg < 8:235887/136‑1 (MQ=255) ttGTGGCAGCAACTGTCTTGCTAAAAATATAAACGGTGCCGCGCCCCCTGGGGGAGCGGGGGGGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCTGCGACCATCGTGTCAg < 2:52298/135‑1 (MQ=255) ggCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGCCGCGCCCCCTGTGGGCGCGGGCGTGGCCGCGCCCACACGGGCGGTGCCACTTTGAAGTCTGTGGGACAACAGGCCCACCAGCGCCCCTCGGGTCAGGccgg > 7:372961/1‑134 (MQ=255) gcaACTGTCTTGCTTAAAATCTAAACGCTGACGCGCCCCCGGGGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCa < 2:284692/135‑1 (MQ=255) aaaaTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTTCCCCCGCCCACAGGGGGGGGGGCAGTTTGAACTGTGGGGGACAACCGGCCCGCCCCCGCCCCACCGGTCCGTCCGTCCCGAAGGGGTTGCGcccg > 8:205958/1‑135 (MQ=255) gcgcccgcgCCCCCTGGGGGCGCGGGCGGGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCggg < 3:417887/130‑1 (MQ=255) gCTGCCGCGCCCCCGGTGGGCGCGGGCGGGCCGGCCCCCAAATGGTCGGTGCAAGTTTCAAGTCTGGGGAACCACAGGCCCTCCAGCGACCATCGTGTCAGGCCTTCCAGAAGGGTTTCCGCCCTTCGATCCggg < 2:12126/135‑1 (MQ=255) ccgcgCCCCCTGTGGGCGCGGGCGCGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGc < 7:205958/135‑1 (MQ=255) cccccTGGGGGCGCGGGCGGGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa < 8:73248/136‑1 (MQ=255) cccgTGGGGGCGCGGGCGGGCCCGCCCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa < 5:134711/135‑1 (MQ=255) ccccTGGGGGCGCGGGGGGCCCCGCCCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa < 1:160898/135‑1 (MQ=255) ccccTGGGGGAGCGGGCGTGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa < 2:89503/135‑1 (MQ=255) | CAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCA > KT2440/6016854‑6017111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |