| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 6,021,514 | T→G | 38.4% | S60R (AGC→CGC) | ycaD_4 ← | putative MFS‑type transporter YcaD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,021,514 | 0 | T | G | 38.4% | 9.5 / 2.4 | 32 | S60R (AGC→CGC) | ycaD_4 | putative MFS‑type transporter YcaD |
| Reads supporting (aligned to +/- strand): ref base T (8/10); new base G (5/6); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC > KT2440/6021379‑6021648 | aCATGCCGATCCTCAGCCGCAGCAGCAGCCATACCGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCt < 8:319125/136‑1 (MQ=255) cATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCt < 3:377414/135‑1 (MQ=255) cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGTCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCggccggccc > 1:94823/1‑134 (MQ=255) cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTCCGCCAACCCAA‑CCTGCCAGGCGGGCGGccc > 3:299635/1‑134 (MQ=255) cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGccc > 5:104763/1‑134 (MQ=255) gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGGGGGcccg > 1:164843/1‑133 (MQ=255) gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGcccg > 1:255881/1‑133 (MQ=255) gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGcccc > 6:390287/1‑133 (MQ=255) catcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTg < 8:220736/136‑1 (MQ=255) aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCCGTACGcccc > 4:160149/1‑134 (MQ=255) gggTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGCCCCTCCGCCCCAcccc > 2:111784/1‑135 (MQ=255) cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCCGGCTGGCCCCCGCCCGTAAGCCCCACCCCCGCCtt > 5:191752/1‑135 (MQ=255) aGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCCGTACGCCCCACGCCGGCATTgc > 3:229119/1‑135 (MQ=255) agcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCGGGGCCGGCGGGCGCCCGGCCGGACGCCCCACGCCCGGCTTggcgcccgc > 1:72401/1‑132 (MQ=255) cgcgAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCGGGCCCGGCGGGCGCCCGCCCGGACGCCCCACCCCCGCATTGCCGCCCTCAc > 5:345206/1‑135 (MQ=255) gAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTGTCGCCGGCCCGTAAGCCCAACCCCCGCCATGACGCCCTAACGcc > 5:231329/1‑135 (MQ=255) ccAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg < 2:245414/135‑1 (MQ=255) cgccccgcccCCCCGGCACAGGCGCAGCGCCGAAGGACCGCCAACCCAC‑CCTGCCCGGCGGGTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAg < 7:324392/136‑1 (MQ=255) gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCGGGCCCCGCTGGCGCCGGGCCGGTCGCCCAACCCCCGGCTTGCCGCCCTCACCCCGATGGCGCAGCTGCCGTAGc > 8:277067/1‑136 (MQ=255) gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCCGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAg > 1:79188/1‑135 (MQ=255) cccgccccACCGCCACCGCCGAAGCCCCGACGG‑ACCCCCACCCAACCCGGCCCGGGGGCTGCCCTGGGGGGGGCCGCCCATAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcc < 1:111784/135‑1 (MQ=255) cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCAGTACGCCCCACGCCGGCCTTGGCCCCCTCACGCCGCTGGCGGAGCTGCCGGAGccccc > 7:299257/1‑135 (MQ=255) ccACCGCCACAGCCGCATCGCCGACGGTACGCCAACCCAA‑CCTGCCCGGCGGCGGGCCAGGGGGGCGCCGGCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGc < 1:257231/135‑1 (MQ=255) acggccacaggcCCTTCCCCGACGTCACGCAAACCCAA‑CGGGCCCGGGGGCGGGCCTGGCGGGGGCGGGACTTTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCt < 6:355656/130‑1 (MQ=255) accccaaacccac‑ccGCCCTGGCGGGGGGCCCGGCGGGCGCGGCCCCGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt < 3:340004/129‑1 (MQ=255) aaCGCCACCCCAC‑CCTGCCCGGCGGCTGGCCAGGCCGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt < 2:127809/134‑1 (MQ=255) aacccaa‑ccTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACTCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCAc < 6:231329/135‑1 (MQ=255) a‑ccGGCCCGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg < 4:145025/135‑1 (MQ=255) cccTGCCCGGGGGCGGCCCCGGGGGGCGCCGGCCCGAACCCCCACCGCCGGCATTGCCGCCAGCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg < 5:298436/134‑1 (MQ=255) gCCCGGCGGCGGGCCAGGCTGGCGCCGGCCATTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa < 7:372319/136‑1 (MQ=255) cgggggcTGGCCCGGGGGGGGCCGCCCTGTCCGCCCAACGCCGGCTTTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc < 3:261693/131‑1 (MQ=255) aGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc < 2:255881/135‑1 (MQ=255) ggcggcAGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGccc > 8:343668/1‑136 (MQ=255) gcggcgGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg < 8:299257/136‑1 (MQ=255) gggcGGGCCAGGGTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg < 6:104763/134‑1 (MQ=255) cTGGCCCGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGacac < 7:277067/136‑1 (MQ=255) | ACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAA‑CCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC > KT2440/6021379‑6021648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |