Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,021,514 T→G 38.4% S60R (AGC→CGC)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,021,5140TG38.4% 9.5 / 2.4 32S60R (AGC→CGC) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (8/10);  new base G (5/6);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC  >  KT2440/6021379‑6021648
                                                                                                                                        |                                                                                                                                      
aCATGCCGATCCTCAGCCGCAGCAGCAGCCATACCGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        <  8:319125/136‑1 (MQ=255)
 cATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        <  3:377414/135‑1 (MQ=255)
      cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGTCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCggccggccc                                                                                                                                   >  1:94823/1‑134 (MQ=255)
      cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTCCGCCAACCCAACCTGCCAGGCGGGCGGccc                                                                                                                                   >  3:299635/1‑134 (MQ=255)
      cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGccc                                                                                                                                   >  5:104763/1‑134 (MQ=255)
       gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGGGGcccg                                                                                                                                  >  1:164843/1‑133 (MQ=255)
       gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGcccg                                                                                                                                  >  1:255881/1‑133 (MQ=255)
       gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGcccc                                                                                                                                  >  6:390287/1‑133 (MQ=255)
          catcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTg                                                                                                                              <  8:220736/136‑1 (MQ=255)
                              aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCCGTACGcccc                                                                                                           >  4:160149/1‑134 (MQ=255)
                                   gggTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGCCCCTCCGCCCCAcccc                                                                                                      >  2:111784/1‑135 (MQ=255)
                                         cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCCCCCGCCCGTAAGCCCCACCCCCGCCtt                                                                                                >  5:191752/1‑135 (MQ=255)
                                           aGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCCGTACGCCCCACGCCGGCATTgc                                                                                              >  3:229119/1‑135 (MQ=255)
                                                  agcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCGGCGGGCGCCCGGCCGGACGCCCCACGCCCGGCTTggcgcccgc                                                                                       >  1:72401/1‑132 (MQ=255)
                                                    cgcgAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCGCCCGCCCGGACGCCCCACCCCCGCATTGCCGCCCTCAc                                                                                     >  5:345206/1‑135 (MQ=255)
                                                       gAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGTCGCCGGCCCGTAAGCCCAACCCCCGCCATGACGCCCTAACGcc                                                                                  >  5:231329/1‑135 (MQ=255)
                                                              ccAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg                                                                           <  2:245414/135‑1 (MQ=255)
                                                                         cgccccgcccCCCCGGCACAGGCGCAGCGCCGAAGGACCGCCAACCCACCCTGCCCGGCGGGTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAg                                                               <  7:324392/136‑1 (MQ=255)
                                                                          gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCGGGCCGGTCGCCCAACCCCCGGCTTGCCGCCCTCACCCCGATGGCGCAGCTGCCGTAGc                                                              >  8:277067/1‑136 (MQ=255)
                                                                          gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCCGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAg                                                               >  1:79188/1‑135 (MQ=255)
                                                                            cccgccccACCGCCACCGCCGAAGCCCCGACGGACCCCCACCCAACCCGGCCCGGGGGCTGCCCTGGGGGGGGCCGCCCATAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcc                                                             <  1:111784/135‑1 (MQ=255)
                                                                              cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCAGTACGCCCCACGCCGGCCTTGGCCCCCTCACGCCGCTGGCGGAGCTGCCGGAGccccc                                                          >  7:299257/1‑135 (MQ=255)
                                                                                  ccACCGCCACAGCCGCATCGCCGACGGTACGCCAACCCAACCTGCCCGGCGGCGGGCCAGGGGGGCGCCGGCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGc                                                       <  1:257231/135‑1 (MQ=255)
                                                                                    acggccacaggcCCTTCCCCGACGTCACGCAAACCCAACGGGCCCGGGGGCGGGCCTGGCGGGGGCGGGACTTTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCt                                                     <  6:355656/130‑1 (MQ=255)
                                                                                                             accccaaacccacccGCCCTGGCGGGGGGCCCGGCGGGCGCGGCCCCGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                            <  3:340004/129‑1 (MQ=255)
                                                                                                             aaCGCCACCCCACCCTGCCCGGCGGCTGGCCAGGCCGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                            <  2:127809/134‑1 (MQ=255)
                                                                                                                   aacccaaccTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACTCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCAc                      <  6:231329/135‑1 (MQ=255)
                                                                                                                         accGGCCCGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  4:145025/135‑1 (MQ=255)
                                                                                                                          cccTGCCCGGGGGCGGCCCCGGGGGGCGCCGGCCCGAACCCCCACCGCCGGCATTGCCGCCAGCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  5:298436/134‑1 (MQ=255)
                                                                                                                              gCCCGGCGGCGGGCCAGGCTGGCGCCGGCCATTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa           <  7:372319/136‑1 (MQ=255)
                                                                                                                                 cgggggcTGGCCCGGGGGGGGCCGCCCTGTCCGCCCAACGCCGGCTTTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc         <  3:261693/131‑1 (MQ=255)
                                                                                                                                 aGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc         <  2:255881/135‑1 (MQ=255)
                                                                                                                                  ggcggcAGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGccc       >  8:343668/1‑136 (MQ=255)
                                                                                                                                   gcggcgGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg      <  8:299257/136‑1 (MQ=255)
                                                                                                                                    gggcGGGCCAGGGTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg      <  6:104763/134‑1 (MQ=255)
                                                                                                                                       cTGGCCCGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGacac  <  7:277067/136‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC  >  KT2440/6021379‑6021648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: