Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,108,673 C→G 8.1% Q249H (CAG→CAC COQ3_3 ← Ubiquinone biosynthesis O‑methyltransferase, mitochondrial

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,108,6730CG 8.1% 139.1 / 5.7 50Q249H (CAG→CACCOQ3_3Ubiquinone biosynthesis O‑methyltransferase, mitochondrial
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base G (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

AGCTCGCCGTGGGGGAATACATAGCGATCGATGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCTTGTCGGCAAGCCCTTC  >  KT2440/6108539‑6108807
                                                                                                                                      |                                                                                                                                      
aGCTCGCCGTGGGGGAATACATAGCGATCGATGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTc                                                                                                                                        <  6:377765/135‑1 (MQ=255)
                aTACATAGCGATCGATGAACTCCCCCGCCCCACGCCCGCCCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCCCCAGCCCCCCGTCCCTCACCGCCCCGCACCGCTTGTGGCAGGACAGCGCCa                                                                                                                        >  6:70036/1‑135 (MQ=255)
                aTACATAGCGATCGATGAACTCCCCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGGGATGCCGTGGTTCATCACCAGCCCCCCTGCCCTCCCCGCCCCGAACAGCATGTGGAAGTACAGCGCCa                                                                                                                        >  6:76761/1‑135 (MQ=255)
                   cATAGCGATCGATGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGt                                                                                                                     <  3:42117/135‑1 (MQ=255)
                    aTAGCGATCGATGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCCGTGATGCCGTGGGTCATCACCACCCCCCCCTCCCGCCCCCCCCCCCACACCTGCTGGCACTACCGCCCCCGGtt                                                                                                                    >  6:31237/1‑135 (MQ=255)
                    aTAGCGATCGATGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGt                                                                                                                     <  6:211330/134‑1 (MQ=255)
                        cgatcgatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGc                                                                                                                 <  1:51167/134‑1 (MQ=255)
                        cgatcgatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGc                                                                                                                 <  5:181115/134‑1 (MQ=255)
                         gatcgatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCCCCAGCCCCCCGTCCCTCCCCGCCCCGAACCGCGTCTGGCAGTACCGCGCCAGGTTGGcct                                                                                                               >  6:403203/1‑133 (MQ=255)
                         gatcgatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCCCCACCCCCCCATCCCGGACCCCCCCGCACAGCTTCTGGGAGTCCAGCGCCAGGATGGCAt                                                                                                               >  1:37580/1‑135 (MQ=255)
                         gatcgatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCGTCCCTCCCCGCCCCGCACAGCTTCTGGCAGGACAGCGCCAGGTTGGCATg                                                                                                              >  7:156480/1‑136 (MQ=255)
                         gatcgatGAACTCACCCGCCCCACGCCCGACCGGTCCGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCATCCCTCGCCGCCCCCCACACCATCGGGCAAGACCGCGCGAGGTTCGCAt                                                                                                               >  6:386293/1‑135 (MQ=255)
                            cgatGAACTCACCCGCCCCACGCCCGACCGGTCGGGCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGcc                                                                                                            <  2:124222/135‑1 (MQ=255)
                             gatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGccc                                                                                                           <  1:96556/135‑1 (MQ=255)
                             gatGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCg                                                                                                          >  8:66754/1‑136 (MQ=255)
                              atGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGccc                                                                                                           <  4:169253/134‑1 (MQ=255)
                              atGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCGTCCCTCACCGCCCCGAACAGCGTCTGGCAGTACAGCGCCAGGTTGGCATGCCCg                                                                                                          >  6:222249/1‑135 (MQ=255)
                                  aCTCACCCGCCCCACGCCCGCCCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACAt                                                                                                      <  6:408972/135‑1 (MQ=255)
                                         cGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGCCATGCGCGaa                                                                                               >  6:141467/1‑135 (MQ=255)
                                             ccACGCCCGACCGGTCGGCCATCGCCGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATa                                                                                           <  1:440225/135‑1 (MQ=255)
                                              cACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATAc                                                                                          <  3:26761/135‑1 (MQ=255)
                                              cACGCCCGACCGGGCGGCCATCGACGTGCTTGGCAGTGATGCCGGGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATAc                                                                                          <  5:303809/135‑1 (MQ=255)
                                                  cccGACCGGGCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCAc                                                                                      <  2:214849/135‑1 (MQ=255)
                                                        cGGTCGGCCAGCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGAc                                                                                <  1:178451/135‑1 (MQ=255)
                                                         ggTCGACCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGcc                                                                                                            >  7:107393/1‑106 (MQ=255)
                                                         ggTCGACCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGcc                                                                                                            <  8:107393/106‑1 (MQ=255)
                                                              gCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGCACAGCTTCTGGCAGTACAGCGCCAGGTTGGCCTGCCCGACATGCTCGAACATACCCACGCTGACAACCtt                                                                          >  5:131226/1‑135 (MQ=255)
                                                              gCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCATCTGGCAGTACAGCGCCAGGTTTGCATGCCCGACATGCTCGAACATACCCACGCTGaccaccat                                                                          >  2:63946/1‑133 (MQ=255)
                                                                      cgtgcgtgGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTGTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  <  5:70036/135‑1 (MQ=255)
                                                                      cGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTGTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  <  5:76761/135‑1 (MQ=255)
                                                                      cGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  <  1:263133/135‑1 (MQ=255)
                                                                      cGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  <  6:327890/135‑1 (MQ=255)
                                                                      cGTGCTTGGCAGTGATGCCGTGGTGCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  <  6:87789/135‑1 (MQ=255)
                                                                            tGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGACATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCagc                                                           >  7:342004/1‑134 (MQ=255)
                                                                                     tGCCGTGGTTCATCACCAGCCCCCCTTCCCTCCCAGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCGCGCACATACCCACGCTGACCACCTTGTCGAAGCGGCCATCCTGGGGCAg                                                  >  8:95363/1‑136 (MQ=255)
                                                                                       ccGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCCCGCTGACAACCTTGTCGACGCGGCCCTCCTGGGGCAgc                                                 >  4:339913/1‑134 (MQ=255)
                                                                                         gTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATc                                               <  4:161189/135‑1 (MQ=255)
                                                                                             ttCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGGAGTACAGCGCCACGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATcgcg                                            >  6:74429/1‑134 (MQ=255)
                                                                                             ttCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGCCATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATcgcgc                                           >  8:334443/1‑134 (MQ=255)
                                                                                                     cAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  <  7:270036/104‑1 (MQ=255)
                                                                                                     cAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGc                                                                  >  8:270036/1‑104 (MQ=255)
                                                                                                          ccccTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTCTCGAAGCGGCCATCCTGGGGCAGCTCGCGATAATCGAGAATCTg                             >  8:130112/1‑136 (MQ=255)
                                                                                                          ccccTTCCCTCACAGCCCCGAACAGCTTATGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACACACGCTGACAACCTTGTCGAAGAGGCCATCCTGGGGCAGATCTCGATAATCGAGAATc                               >  5:222367/1‑134 (MQ=255)
                                                                                                             cTTCCCTCACCGCCCCGAACAGCTGCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCa                           <  5:100439/135‑1 (MQ=255)
                                                                                                                cccTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTTGCATGCCCGACATGCTCGCACATACCCACGCTGACAACCTTGTCGAAGCGGCCCTCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGt                        >  5:387940/1‑135 (MQ=255)
                                                                                                                cccTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTc                       >  8:79014/1‑136 (MQ=255)
                                                                                                                   tCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGa                     <  6:232817/135‑1 (MQ=255)
                                                                                                                      ccGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCt                  >  5:165180/1‑135 (MQ=255)
                                                                                                                         ccccGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTTGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCTTGTc              <  7:357212/136‑1 (MQ=255)
                                                                                                                          cccGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCTTGTCg             >  8:144882/1‑136 (MQ=255)
                                                                                                                             gAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATAGCGATAATCGAGAAACTGCAGGTCGACATTGTCgg            >  3:422362/1‑134 (MQ=255)
                                                                                                                                      cTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCTTGTCGGCAAGCCCTTc  >  4:188377/1‑135 (MQ=255)
                                                                                                                                      cTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCTTGTCGGCAAGCCCTTc  >  3:90847/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                      
AGCTCGCCGTGGGGGAATACATAGCGATCGATGAACTCACCCGCCCCACGCCCGACCGGTCGGCCATCGACGTGCTTGGCAGTGATGCCGTGGTTCATCACCAGCCCCCCTTCCCTCACCGCCCCGAACAGCTTCTGGCAGTACAGCGCCAGGTTGGCATGCCCGACATGCTCGAACATACCCACGCTGACAACCTTGTCGAAGCGGCCATCCTGGGGCAGATCGCGATAATCGAGAATCTGCAGGTCGACCTTGTCGGCAAGCCCTTC  >  KT2440/6108539‑6108807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: