Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 395,021 A→C 32.6% P185P (CCT→CCG)  yibF ← putative GST‑like protein YibF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440395,0210AC32.6% 46.5 / 3.7 53P185P (CCT→CCG) yibFputative GST‑like protein YibF
Reads supporting (aligned to +/- strand):  ref base A (28/6);  new base C (11/8);  total (39/14)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTG  >  KT2440/394894‑395147
                                                                                                                               |                                                                                                                              
acaGATAGCGACCACTCTGGAAAACCTTGTACGAGCGGGTTGACCAGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGCCCACCACCGCCTACCCCGCCGCCAACCCAgccagtc                                                                                                                         >  1:314228/1‑129 (MQ=255)
acaGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGgcg                                                                                                                        >  7:190059/1‑136 (MQ=255)
acaGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCACGGCATACCACGCCGCCACCCCAGCCTGgc                                                                                                                         >  5:19103/1‑135 (MQ=255)
         gAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACgcc                                                                                                                >  4:167103/1‑135 (MQ=255)
            cacTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCCCCTCGGCATACCACCCCGCCCACCCCGGCAGGCCCACCCgccccc                                                                                                             >  2:438128/1‑132 (MQ=38)
            cacTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCCCGCCGCCACCCCAGGCTGGCGCTCACgccagc                                                                                                             >  4:370745/1‑135 (MQ=255)
            cacTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACgccagc                                                                                                             >  2:52330/1‑135 (MQ=255)
               tgtgGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGCCCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCCACCCCGGCTGGCGCTCCCGCCCGCCaaa                                                                                                         >  8:304751/1‑136 (MQ=39)
                 tgGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCTCTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGGCGCTCACGCCAGCCAAAc                                                                                                        >  1:278500/1‑135 (MQ=255)
                 tgGAAAACCTTGTAGGAGCGGGTTAACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCAAAGAAGGCCGCTTGACAACCTCGGCATACCACGCCGCCAAACCAGGCTGGCGCTCACGCCCGCCacac                                                                                                        >  5:110009/1‑135 (MQ=255)
                   gAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGCCCATCGATGGGCGCGTGCCCACCTAGGCATACCCCGCCGCCAACCCAGCAAGGCGCTCACGCCAGCCACAccc                                                                                                      >  1:88214/1‑135 (MQ=38)
                   gAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGCCCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCg                                                                                                     >  8:122812/1‑136 (MQ=255)
                   gAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAAccc                                                                                                      >  1:341842/1‑135 (MQ=255)
                   gAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCg                                                                                                     >  8:146199/1‑136 (MQ=255)
                   gAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCACGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAAccc                                                                                                      >  4:188707/1‑135 (MQ=255)
                    aaaaCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCAGGCGCTCCCGCCCGCCAAACCCgg                                                                                                    >  7:105291/1‑136 (MQ=255)
                    aaaaCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCGTGACCCCCTCGGCATACCACGCCGCCCACCCCGGCTGGCGCTCACCCCCGCCCAAcccc                                                                                                     >  5:260486/1‑134 (MQ=38)
                    aaaaCCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGAACATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGGCGCTAACGCCCGCCAAACCCgc                                                                                                    >  7:315024/1‑135 (MQ=255)
                              aGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCCCGCCGCCAACCCCGGCTGGCGCTCACGCCAGCCAAACCCGGGCGGCCGcc                                                                                           >  7:19121/1‑134 (MQ=255)
                                   cGGTTTTCCCCGCGACGGGGCCCGCCGGCCACTGGCGCTTTAGCGACCTTCGATGGGCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATcc                                                                                      <  3:184165/135‑1 (MQ=255)
                                   cGGGTTACCCCGCGAGGGGGCCCTCCGGCCAGTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCAAACCCCGCCGCCAACCCCGGGGGGCGCACACGCCAGCCAAACCCGGGCTGACGCAAATcc                                                                                      <  2:341842/135‑1 (MQ=255)
                                     ggTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCACCCCAGGCTGGCGCTCACGCCCGCCAAACCCGGGCTGACGCACAt                                                                                        >  2:357904/1‑131 (MQ=255)
                                      gTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGAGCACCTCGGCAGACCCCGCCGCCAACCCAGGCAGGCGCTCACGCCAGCCCAACCCGGGCTGCGGCCAATCCAGa                                                                                   >  1:406023/1‑135 (MQ=255)
                                      gTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCCCCTCGGCATACCACGCCGCCAACCCCGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCaca                                                                                   >  7:411490/1‑133 (MQ=255)
                                      gTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCCCGCCGCCACCCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAg                                                                                    >  1:286863/1‑134 (MQ=255)
                                              ggtaaGGGGCCCTCTGGCCAGTGGCGCTGTAGCTACCATCGATGGCCGCTTGACCACGTCGCCATACCACGCCCCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAgc                                                                          <  7:304768/133‑1 (MQ=255)
                                                  aGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCCCCTCGGCATACCCCGCCGCCAACCCCGGCTGGCGCGCCCGCCAGCCAAACCCGGGCTGCCGCAAATCCAGATAgcccagccca                                                                       >  1:365916/1‑135 (MQ=255)
                                                               ccACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCACCCCAGGCTGGCGCTCACGCCCGCCACACCCGGGCTGACGCAAATCCAGATAGCCCCGCGCCCAGGCCACGCCaa                                                          >  6:208540/1‑135 (MQ=255)
                                                                      cgcTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATcgccgg                                                   >  4:135702/1‑134 (MQ=255)
                                                                      cgcTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATcgccg                                                    >  2:340512/1‑134 (MQ=255)
                                                                             gctaCCATCGATGGCCGCTTAACCACCTCGCAAAACCCCGCCGCAAACCCAGGGTGGCGCTCACCCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGa                                           <  8:315024/133‑1 (MQ=255)
                                                                              cGACCATCGAGGGCCGCTTCCCCCCCCCGCCAACCCCCCCCCCCACCCCCGGCGGGCGCCCCCGCCACCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGa                                           <  3:296277/135‑1 (MQ=255)
                                                                               gACCATCGATGGCCGCTTGACCACCTCGTCAGACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAg                                         <  8:231488/136‑1 (MQ=255)
                                                                                aCCATCGATGGCCGCTTGCCCCCCGCGGAATACCCCGCCGCCAACCCCGGCGGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAg                                         <  5:210699/135‑1 (MQ=255)
                                                                                  cATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCCGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGcc                                       >  1:369152/1‑135 (MQ=255)
                                                                                   aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCg                                      >  3:6002/1‑135 (MQ=255)
                                                                                   aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCg                                      >  5:194165/1‑135 (MQ=255)
                                                                                   aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACCCCAATCGCCGCCAGGTCGAAGCCgg                                     >  7:194154/1‑136 (MQ=255)
                                                                                        tGGCCGCTTGCCCACCTCGGCATACCCCGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCCGGCTGACGCAAATCCAGATAGCCCAGCGCCCCGGCCACGCCAAACGCCGCCACGTCGAAGCCGgaga                                  >  1:189590/1‑134 (MQ=255)
                                                                                        tGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGgaga                                  >  6:308877/1‑134 (MQ=255)
                                                                                              cTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGCCGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGagctcagctc                           >  2:161716/1‑135 (MQ=255)
                                                                                               tcccccaccGCGGAAACCCCCGCCGCCACCCCCGGCGGCCCCGCCCGCCACCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTcc                          <  5:208540/131‑1 (MQ=255)
                                                                                                  acccccTCGCCAACCCCCCCCCCCAACCCCGGCTGGCGCCCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGc                       <  7:146199/135‑1 (MQ=255)
                                                                                                     accTCGCCATCCCACGCCGCCAACCCCGGCTGGCGCTCCCGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGGCGAAGCCGGAGATCAGCTCCGGCAAg                    <  5:76681/135‑1 (MQ=255)
                                                                                                       cTCGGCATCCCACGCCCCCACCCCCGGCGGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtg                  <  2:369152/135‑1 (MQ=255)
                                                                                                          ggCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCCGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctc               >  2:387412/1‑135 (MQ=255)
                                                                                                          ggCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctc               >  4:362665/1‑135 (MQ=255)
                                                                                                                 cACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctgctcc        <  4:274474/135‑1 (MQ=255)
                                                                                                                    gccgccAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAgg     >  6:182069/1‑135 (MQ=255)
                                                                                                                     ccgccAACCCAGGCGGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGtt   <  7:323/136‑1 (MQ=255)
                                                                                                                      ccccACCCCCGGCGGGCGCTCACGCCCGCCAAACCCCGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGtt   <  3:288765/133‑1 (MQ=255)
                                                                                                                       gccAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTg  >  3:355346/1‑135 (MQ=255)
                                                                                                                       gccAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTg  >  4:369684/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                              
ACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTG  >  KT2440/394894‑395147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑