Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	491,817	T→A	5.1%	L81M (TTG→ATG)	uvrY →	Response regulator UvrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	491,817	0	T	A	5.1%	232.8 / 3.0	79	L81M (TTG→ATG)	uvrY	Response regulator UvrY
Reads supporting (aligned to +/- strand):  ref base T (45/30);  new base A (2/2);  total (47/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

AGTTGCTCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATT  >  KT2440/491689‑491951                                                                                                                                 |                                                                                                                                       aGTTGCTCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGa                                                                                                                                  >  1:76035/1‑135 (MQ=255) aGTTGCTCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGAt                                                                                                                                 >  8:128772/1‑136 (MQ=255)  gTTGCTCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGAt                                                                                                                                 <  6:242222/135‑1 (MQ=255)      cTCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCAc                                                                                                                             <  3:272159/135‑1 (MQ=255)       tCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCAc                                                                                                                             <  3:110008/134‑1 (MQ=255)       tCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACg                                                                                                                            <  2:252265/135‑1 (MQ=255)       tCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACg                                                                                                                            <  2:439148/135‑1 (MQ=255)                   gcCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGa                                                                                                                >  6:103796/1‑135 (MQ=255)                   gcCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGAGGTTGTCGATGCACGATGAGGCACAGa                                                                                                                >  1:441722/1‑135 (MQ=255)                          acCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATggcggc                                                                                                         >  3:251525/1‑135 (MQ=255)                           ccGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGgcgc                                                                                                       >  8:385729/1‑136 (MQ=255)                            cGTGCGAGGGGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGgcgc                                                                                                       <  5:138285/135‑1 (MQ=255)                               gCGAGGTGGTACTGCTGGACATCTCGATTCAGTGTGTGAACGGCCTGGAGGCGATCACGCGTATCCTTGCGCTGCACGATGCTCAAGCGAGCCTGATGTTGTCGATGCACTATGAGTCACAGATGGCGGCGCggg                                                                                                    >  2:360242/1‑135 (MQ=255)                               gCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCggg                                                                                                    >  5:17724/1‑135 (MQ=255)                               gCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCggg                                                                                                    >  3:443522/1‑135 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcgc                                                                                                 >  7:379614/1‑136 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcgc                                                                                                 >  7:113686/1‑136 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcg                                                                                                  >  1:154138/1‑135 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcg                                                                                                  >  5:390869/1‑135 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcg                                                                                                  >  6:69216/1‑135 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcg                                                                                                  >  1:96033/1‑135 (MQ=255)                                 gAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCAAGCGATCCGGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcg                                                                                                  >  6:87795/1‑135 (MQ=255)                                  aGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcgc                                                                                                 >  3:103791/1‑135 (MQ=255)                                  aGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGgcgc                                                                                                 >  3:409706/1‑135 (MQ=255)                                       gtACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGAACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCTGCGCGGGCGCTGAAg                                                                                            >  4:117678/1‑135 (MQ=255)                                                gACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTgcgg                                                                                  >  8:320467/1‑136 (MQ=255)                                                  cATCTCGATTCAGGGTGTGAACGGCCTGTAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCGCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAGGGCCGGTgcggc                                                                                 >  5:952/1‑135 (MQ=255)                                                  cATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTgcggc                                                                                 >  1:432687/1‑135 (MQ=255)                                                  cATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTgcggc                                                                                 <  5:103796/135‑1 (MQ=255)                                                     ctcGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTgcggc                                                                                 >  6:176160/1‑132 (MQ=255)                                                     ctcGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTgcggc                                                                                 <  5:176160/132‑1 (MQ=255)                                                     ctcGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCggg                                                                              >  6:295983/1‑135 (MQ=255)                                                     ctcGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGc                                                                             >  8:362858/1‑136 (MQ=255)                                                     ctcGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTAATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCggg                                                                              >  2:154414/1‑135 (MQ=255)                                                     ctcGATGCCGGGTGTGAACGGCCTGGAGGCGATCCAGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCggg                                                                              >  6:120782/1‑135 (MQ=255)                                                        gATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCt                                                                            <  7:128772/134‑1 (MQ=255)                                                        gATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTa                                                                           >  1:394511/1‑135 (MQ=255)                                                        gATGCCGGGTGTGAACGGCCTGGACGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGCCTa                                                                           >  2:360113/1‑135 (MQ=255)                                                        gATGCCGGCTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGAGGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTAt                                                                          >  8:249240/1‑136 (MQ=255)                                                         ctGCCGGGTGTGAACGGCCTGGAGGCGATCACGCGTATCCGTGCGCTGCAGGATGCTCCAGCTATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCt                                                                            <  3:34744/132‑1 (MQ=255)                                                         aTGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCggg                                                                              <  1:380109/131‑1 (MQ=255)                                                         aTGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCt                                                                            <  3:363317/133‑1 (MQ=255)                                                         aTGCCGGGGGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCt                                                                            <  2:96033/133‑1 (MQ=255)                                                         aTGCCGGGGGGGAACGGCCTGGAGGCGAGCCCGCGTATCCGGGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCt                                                                            <  5:276908/133‑1 (MQ=255)                                                               ggTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCTGGCTATGccacca                                                                   >  8:427412/1‑136 (MQ=255)                                                                gtgtGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCaa                                                                  <  7:427412/136‑1 (MQ=255)                                                                   tgAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAg                                                                >  5:411001/1‑135 (MQ=255)                                                                   tgAACGGCCTGGAGGCGATCCCGCGTATCCGGGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAg                                                                <  3:237664/135‑1 (MQ=255)                                                                             ggAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGCTGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGCCAGTGATcc                                                      >  6:21201/1‑135 (MQ=255)                                                                             ggAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATcc                                                      >  3:190601/1‑135 (MQ=255)                                                                             ggAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATcc                                                      >  3:218140/1‑135 (MQ=255)                                                                             ggAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCg                                                     >  8:385470/1‑136 (MQ=255)                                                                              gtggCGATCCGGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCg                                                     <  6:4750/133‑1 (MQ=255)                                                                              gAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCg                                                     <  7:79850/135‑1 (MQ=255)                                                                                     tCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCgttgtt                                             <  7:223038/136‑1 (MQ=255)                                                                                       ccGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCgttgttg                                            >  2:322278/1‑135 (MQ=255)                                                                                       ccGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCgttgttg                                            >  6:267876/1‑135 (MQ=255)                                                                                       ccGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCgttgtt                                             <  2:76035/134‑1 (MQ=255)                                                                                                  tGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCCCCAAAGCCAGTGATCCGGCGTTGTTGTTGACAGCGAtt                                >  7:252738/1‑136 (MQ=255)                                                                                                   gcgcTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGAtt                                <  1:297839/135‑1 (MQ=255)                                                                                                          aCGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGt                        >  7:6209/1‑136 (MQ=255)                                                                                                            gATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTg                       >  6:184841/1‑135 (MQ=255)                                                                                                                    aGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGt                 <  6:311409/133‑1 (MQ=255)                                                                                                                    aGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGt                 <  4:254562/133‑1 (MQ=255)                                                                                                                    aGCGATCCTGATGATGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGt                 <  4:104070/133‑1 (MQ=255)                                                                                                                    aGCGATCCTGATGATGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGt                 <  4:110321/133‑1 (MQ=255)                                                                                                                    aGCGATCCTGATGATGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTgg               >  3:104070/1‑135 (MQ=255)                                                                                                                    aGCGATCCTGATGATGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTgg               >  3:110321/1‑135 (MQ=255)                                                                                                                      cGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg            <  8:113686/136‑1 (MQ=255)                                                                                                                      cGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg            <  8:273259/136‑1 (MQ=255)                                                                                                                       gATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg            <  6:411001/135‑1 (MQ=255)                                                                                                                       gATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg            <  4:263644/135‑1 (MQ=255)                                                                                                                       gATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg            <  3:124393/135‑1 (MQ=255)                                                                                                                       gATCCTGAGGTGGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCTGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTggcgg            <  4:299914/135‑1 (MQ=255)                                                                                                                           cTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGCCAGCGATTCGTCGGGTGGCCGGTggcgggcg         >  5:190482/1‑134 (MQ=255)                                                                                                                           cTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGt        >  5:42489/1‑135 (MQ=255)                                                                                                                             gATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATc                                                       <  8:331479/86‑1 (MQ=255)                                                                                                                             gATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATc                                                       >  7:331479/1‑86 (MQ=255)                                                                                                                              aTGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTtata    >  7:159351/1‑136 (MQ=255)                                                                                                                                 ttgtCGAGGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATAtt  <  7:201335/135‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       AGTTGCTCGAAACCCTGCGCCACACACCGTGCGAGGTGGTACTGCTGGACATCTCGATGCCGGGTGTGAACGGCCTGGAGGCGATCCCGCGTATCCGTGCGCTGCACGATGCTCCAGCGATCCTGATGTTGTCGATGCACGATGAGGCACAGATGGCGGCGCGGGCGCTGAAGGCCGGTGCGGCGGGCTATGCCACCAAAGACAGTGATCCGGCGTTGTTGTTGACAGCGATTCGTCGGGTGGCCGGTGGCGGGCGTTATATT  >  KT2440/491689‑491951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑