Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	495,400	A→G	100%	V213V (GTA→GTG)	CJ019_00444 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	495,400	0	A	G	100.0%	269.7 / ‑6.8	73	V213V (GTA→GTG)	CJ019_00444	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (48/24);  minor base C (1/0);  total (49/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTACTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGTGCTGCTGCA  >  KT2440/495269‑495531                                                                                                                                    |                                                                                                                                    ctgGCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTgg                                                                                                                                 <  8:253204/136‑1 (MQ=255) ctgGCATCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCAACCCGGACCAGGCCATTTACATGGACATTTTTGTCCTTACCCTCTGGATGGGAGTGGTGAGTACCACTATCTGCCTGGTGCTgg                                                                                                                                 >  8:109518/1‑136 (MQ=255)    gCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCt                                                                                                                              >  7:51283/1‑136 (MQ=255)    gCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTCGACATTTTTGTCCGTCCCCTGTGGATGGGCGTGGTGATTCCCACTATCTGCCTGGTGCTGGc                                                                                                                                >  2:256047/1‑134 (MQ=255)      cTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTAc                                                                                                                            <  8:70490/136‑1 (MQ=255)       tCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTAc                                                                                                                            <  4:290920/135‑1 (MQ=255)       tCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCAACCCGGACCATGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGTCGTGGTGATTTCCACTATCTGACTGGTGCTGTCCTAc                                                                                                                            <  5:420305/135‑1 (MQ=255)          gTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTAcc                                                                                                                           <  5:266506/133‑1 (MQ=255)           tGGACCATCGCAGCGATGACCTTGGTGAGCGAGCCAAGGCCACCCCGGACCCGGCCATTTCCCTTGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACt                                                                                                                       <  8:404419/136‑1 (MQ=255)            ggACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTa                                                                                                                             >  7:146470/1‑129 (MQ=255)            ggACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTa                                                                                                                             <  8:146470/129‑1 (MQ=255)            ggACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACt                                                                                                                       <  2:81781/135‑1 (MQ=255)                 atcgcatcgATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCt                                                                                                                  >  3:432714/1‑135 (MQ=255)                 atcgcatcgATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTa                                                                                                                 <  8:118358/136‑1 (MQ=255)                   cgcatcgATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTAc                                                                                                                >  4:176861/1‑135 (MQ=255)                   cgcatcgATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTAc                                                                                                                >  5:268652/1‑135 (MQ=255)                      atcgATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACttg                                                                                                             >  2:334791/1‑135 (MQ=255)                          aTGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGc                                                                                                        <  8:246520/136‑1 (MQ=255)                             aCCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCa                                                                                                      >  2:225144/1‑135 (MQ=255)                                   gTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGc                                                                                                >  4:266311/1‑135 (MQ=255)                                    tGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGc                                                                                                <  4:308103/134‑1 (MQ=255)                                           gCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTCCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGACGACCCgg                                                                                        >  7:54010/1‑135 (MQ=255)                                           gCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCAGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGACAACCTGCCGCCCCgg                                                                                        >  8:112020/1‑135 (MQ=255)                                            ccAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCg                                                                                         <  1:168555/133‑1 (MQ=255)                                               aGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGCCCCGGCagag                                                                                   >  8:254835/1‑136 (MQ=255)                                                 gCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGc                                                                                  <  2:420730/135‑1 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCGGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGACTACCCACTGGCCTACTAGTTGGCCAACCTGCCGACCCGGCAGAGCAACctgc                                                                           >  4:162994/1‑135 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCCCTGGCCTACTTGTTGGCCACCCTGCCGACCCGGCAGAGCACCcggc                                                                           >  6:5531/1‑135 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGCGCAACcggct                                                                          >  8:228261/1‑136 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCCACctgc                                                                           >  6:159970/1‑135 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACctgct                                                                          <  8:366154/136‑1 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACctgc                                                                           >  3:364111/1‑135 (MQ=255)                                                        cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACctgc                                                                           >  4:180935/1‑135 (MQ=255)                                                              aGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTgatgat                                                                    >  8:61250/1‑136 (MQ=255)                                                                  cATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCt                                                                 >  2:446468/1‑135 (MQ=255)                                                                  cATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCt                                                                 >  1:228148/1‑135 (MQ=255)                                                                  cATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTg                                                                >  8:127615/1‑136 (MQ=255)                                                                   aTTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTg                                                                >  3:130595/1‑135 (MQ=255)                                                                        ccTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGc                                                            <  6:268652/134‑1 (MQ=255)                                                                               aTTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGccc                                                    >  3:408405/1‑135 (MQ=255)                                                                                              cTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATc                                     >  1:367143/1‑135 (MQ=255)                                                                                                  ggATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTgg                                 >  1:393827/1‑135 (MQ=255)                                                                                                   gATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCCCTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTGTGGACATCGCTCCTGGt                                >  1:160579/1‑135 (MQ=255)                                                                                                   gATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTa                               >  7:146553/1‑136 (MQ=255)                                                                                                    aTGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTa                               >  1:108676/1‑135 (MQ=255)                                                                                                    aTGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTa                               >  3:33862/1‑135 (MQ=255)                                                                                                    aTGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTAc                              >  7:275301/1‑136 (MQ=255)                                                                                                       ggCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGa                            >  5:194447/1‑135 (MQ=255)                                                                                                        gCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGa                            <  2:357769/134‑1 (MQ=255)                                                                                                         cGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGc                                                            >  4:74126/1‑101 (MQ=255)                                                                                                         cGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGc                                                            <  3:74126/101‑1 (MQ=255)                                                                                                          gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGCCCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTg                         >  5:9339/1‑135 (MQ=255)                                                                                                          gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCAATGGCCTACTTGTTGGCCAACCAGCCGACCCGGCAGAGCAACCTTCTGATGATTCTGGTGCTGTTGCACTTCTGGACATCGAAACTGGTACGAGTg                         >  6:54628/1‑135 (MQ=255)                                                                                                            ggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGCTCCTGGTACGAGTggcg                      >  7:376721/1‑136 (MQ=255)                                                                                                              tgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcggc                    >  7:288301/1‑136 (MQ=255)                                                                                                              tgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcgg                     >  5:347086/1‑135 (MQ=255)                                                                                                              tgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcg                      <  6:347086/134‑1 (MQ=255)                                                                                                                 ttACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTATGAGTGGCGGCGt                  <  3:226557/135‑1 (MQ=255)                                                                                                                 ttACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGt                  <  4:33862/135‑1 (MQ=255)                                                                                                                 ttACCACTATCTGCCTGGGGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGt                  <  1:360790/135‑1 (MQ=255)                                                                                                                  tACCACTATCTGCCGGGGGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTg                 <  1:195626/135‑1 (MQ=255)                                                                                                                     cACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCg                                        <  1:223090/109‑1 (MQ=255)                                                                                                                     cACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCg                                        >  2:223090/1‑109 (MQ=255)                                                                                                                     cACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATc             >  8:25745/1‑136 (MQ=255)                                                                                                                             gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGtgctgct     >  7:316661/1‑136 (MQ=255)                                                                                                                             gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGtgctgc      >  4:305094/1‑135 (MQ=255)                                                                                                                             gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGtgctgc      >  5:355549/1‑135 (MQ=255)                                                                                                                             gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGtgctgc      >  2:54712/1‑135 (MQ=255)                                                                                                                             gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGtgctgc      >  4:251552/1‑135 (MQ=255)                                                                                                                               cTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATAGtgctgct     >  1:292861/1‑134 (MQ=255)                                                                                                                                 ggTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTg                                                          >  1:53814/1‑79 (MQ=255)                                                                                                                                 ggTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTg                                                          <  2:53814/79‑1 (MQ=255)                                                                                                                                 ggTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGTGGGGCTGCa  >  5:252513/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                    CTGGCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTACTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGTGCTGCTGCA  >  KT2440/495269‑495531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑