| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 96,480 | T→G | 35.1% | intergenic (+121/‑72) | cynT → / → CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 96,480 | 0 | T | G | 35.1% | 33.1 / 6.4 | 48 | intergenic (+121/‑72) | cynT/CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/13); new base G (8/8); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTGGTGG > KT2440/96348‑96609 | ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGGTGGCGGCCCGCGCCGgc > 6:354326/1‑135 (MQ=255)ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgcg > 7:86749/1‑136 (MQ=255)ggCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc < 3:271050/135‑1 (MQ=255) gCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgcg < 1:125991/135‑1 (MQ=255) gCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCGGCCGTGAATTACCTGCCTGCCCGGCCGGCGTGGGCGCGGGCGGCCCCCGCCGgcgggg > 1:105382/1‑132 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGGCGTCGGCGGGCTTGACTTCCCTGACAGCCCTGCCGGCGGGCTCGCGGGCGGCCCCCCCCCgggcgcgg > 6:46418/1‑133 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCGGGCGTGAATTACCAGACTTCCCTGCCGGCGTGGGCGCTGGCGGCCCCCGGCCgcgcgcgt > 6:24520/1‑133 (MQ=255) ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGCCTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGGGTGTc > 8:140492/1‑136 (MQ=255) ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTc > 7:324784/1‑136 (MQ=255) ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCGCGCTGGCGGCCCCCGCCGgggcgcgttg > 1:350672/1‑131 (MQ=255) ccGTCCGCCGCTAGAACGTTTATAGCCGGCTGCACCCTTCTGGGGGGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGGGGGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCaa < 2:236544/135‑1 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTACCTGACTTCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCCCGGCGCGCGTGTCAGGGGCaaaa > 4:62012/1‑134 (MQ=255) gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCGGGCGTGAATTCCCTGCCTGCCCTGCCGGCGTGGTCGCTGGCGGCCCGCGCCGGGGCGCTTGTCAGGGGccacc > 6:446573/1‑131 (MQ=255) ccgccgCTAGAACCTTGATAGCCGGGTGCACCCTTCCGGGGGGTGGCGCGGCCTTCGGCTGCCTTGAATACCCTGACTGCCCGGCCGGCGTGCTCGCTGGCGGCCCGCGCGTGCGCGCTTGTCAGGGGCAAACGTg < 8:424563/136‑1 (MQ=255) tAGACCCTTGATAGCCGGCTGCACCCTTCAGGGGTGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGGGCTTGTCAGGGGCAAACGTGCCCACg < 3:355915/135‑1 (MQ=255) aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGGATgg > 7:299396/1‑136 (MQ=255) gCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGc < 8:86749/136‑1 (MQ=255) cTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCGCAGCGCGCATGTCAGGGGGAAAACTGCCCCCCGGCAGGGGAAGGGGCGTgg > 7:351621/1‑135 (MQ=255) tGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCCCGGCCAGGGGAATGGCCGTGc > 2:280983/1‑135 (MQ=255) ccTTCATGGGTGTGGCGCGGCCTTCGGCTGCCTTTAAATCCCTCACTGCCCTGCGGGCGTGCTCGCTGTGGGCCCGCGCCTGCGGGGTTGTCTGGGGAAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTag < 7:28/136‑1 (MQ=255) aGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTTCCCAAGGCCAGGGGTATGGCCGTTCGACTAtaga > 8:298875/1‑134 (MQ=255) ggggTGTGGCGCGGCCTTAGGCTGGCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACCGCCACGGGAATGGCCGGGGGCCGAGAGaaa > 4:191021/1‑135 (MQ=255) gcggGGCGTTCGGGTGCCTTTAATTCCCTGACTGCCCTGCGGGGGTGCGCGCGGGGGGCCCGCGCGGGCGCGCTTGTCGGGGGAAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAac < 5:24520/135‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCGTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:157353/136‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCt > 8:10331/1‑66 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCt < 7:10331/66‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:360869/136‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCGCCTGCGCGCTTGGCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGGGGGCCTAGCGAAAGGAGAAaca > 2:338562/1‑135 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTACCTGACAGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGGGCGCTTGTGAGGGGCAAACGTGCCCACGGCCAGGGGCATGGCCGTGCGACATGCGCAAGGAGAAaca > 6:408104/1‑135 (MQ=255) cgcgGCCTTCGGCGGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGGGGCCCCCGCCGGCGCGCGTGTCAGGGGGCAACCTGCCCCCCGCCCGGGGGATGGCCCGGCGACGAGAGGAAGGCGCAaca > 6:138089/1‑135 (MQ=255) cgcgGCCTTAGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCAGCGCGCGTGTCAGGGGCAAACCTGCCCACCGCCACGGGCATGGCCGTGCGCCTATAGAAAGGAGCAacac > 8:251313/1‑136 (MQ=255) gcgGCCTTCGGCGTCCTTTAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGGGCGGGGGGGCTTGGCTGGGGAAACCGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 5:7073/135‑1 (MQ=255) gcgttCGGCTGCCTTTAATTCCCTGACGGCCCTGCGGGCGGGCTGGGTGGGGGCCCGCGCCGGCGCGCTTGTCTGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑Gt < 3:104947/132‑1 (MQ=255) cTTGGGCTGCCTTTAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGGGGCCCGCGCGGGCGCGGTGGTCAGGGGCAAACGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGa < 5:354326/135‑1 (MQ=255) ggCTGCCTTGATTTCCCTGACTGCCCTGCCGGCGGGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGAAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACAtt < 7:251313/136‑1 (MQ=255) gcggCCTTAAATTCCCTGACTCCCCTGCCGGGGTGCGCGGGGGGGGCCCGCGCCGGCGGGCTTGTCTGGGGCAACCGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACAtt < 5:42598/132‑1 (MQ=255) gCTGCCTTGAATTCCGTGCGTGCCCGGCGGGCGGGCGCGCTGGCGGCCCGCGCCGGCGCGGTGGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACAtt < 2:91218/135‑1 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTACACAAAGGAGAAACACG‑GTGAACATTACACAGt > 3:106834/1‑135 (MQ=255) tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGGGCGCCTAGAGAAAGGAGAAACACG‑GGGAACAATACACAGt > 3:86281/1‑135 (MQ=255) gACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGGGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCGCTGAGACATGAGAAAGTAGAACCACG‑GTGAACATTAGACCGTTGAAGGAGGc > 5:435967/1‑135 (MQ=255) gACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCCCTGCGCGCTTGTGCGGGGCCAACCTGCCCCCCGCCAGGGGAATGGCCCTGCGCGAAGAGCACGGAGAAACACC‑GTGCACAGTACCCAGTAGCAAGCGGc > 6:358678/1‑135 (MQ=255) tgccGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTTCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAACGGAGCAACACG‑GGGTACATTAACCAGTTGACAGCGGCCCTGCcgcg > 8:214902/1‑136 (MQ=255) gCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAGGGGCAAACAGGGCCACGGCCAGGGGTATGGCCGTGGGACGAGAGAAAAGCTAAACACGCGGGACCATT‑CTCCGTTGGAAGCGGCCCGGGcgcgggcg > 4:236300/1‑131 (MQ=255) cgcgggggcgccCCGCGCCGCCGCGGTGGGCCGGGGAAAAGGTCCCCAGGGCCAGGGGAAGGGCCGTGCGACTAGAGAAAGGAGAAACAGG‑GTTAACATAACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCt < 6:382513/129‑1 (MQ=255) cTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGCCGAGAGAAAGGAGAAACACG‑GTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCt > 4:113132/1‑135 (MQ=255) tCGCGGGCGGCCCGCGCCTGCGCGCTTGTCGGGGGCAACCGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTgg < 8:324784/136‑1 (MQ=255) gtGGGGGCCGGCGCGGGCGGGCTTTGCATGGGAAAAGGTGCCCACGGCCAGGGTATGGGCTGTGCGACTTTATAAAGCACAAACAGG‑GTTAACATTACAAAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGt < 8:351621/135‑1 (MQ=255) cggcCCGGGCGTGCGGGTTTGGCTGGGGAAAAGGTGCCCAGGGCCTGGGGATGGGCCGTGCGACTACACAAAGGAGAAACCCG‑GTCAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCgg < 4:64345/135‑1 (MQ=255) ccGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggtggt > 4:411945/1‑135 (MQ=255) gcgcGTGCGCGCTGGGCAGGGGAAACCGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggtggtgg < 2:350672/135‑1 (MQ=255) | GGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACG‑GTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTGGTGG > KT2440/96348‑96609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |