Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	930,465	A→C	21.0%	V2736G (GTG→GGG)	CJ019_00881 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	930,465	0	A	C	21.0%	90.4 / 3.4	61	V2736G (GTG→GGG)	CJ019_00881	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/26);  new base C (13/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 6.06e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCGCTGGCCCCCACCACGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCC  >  KT2440/930331‑930592                                                                                                                                       |                                                                                                                                cgccgcGGGCCCCCACCACGACACTGCCCAGTACAGTGGTGCCAGCGGCGGCCAGCACACGGACGGTGCGGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCcac                                                                                                                                <  8:239674/136‑1 (MQ=255)         gCCCCCACCACGACACGGCCCAGTACAGGGGTGCCAGCGGCGGCCAGCACTCGGAGGGTGCTGCCTGCTTGGGGGCGGCCGCGGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGcgcg                                                                                                                         <  2:302207/135‑1 (MQ=255)                            ccAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGTACCGTCGCCACTGATTGCCAGATCGCGTGGCGCCCCAGGCGCGGTGATGTCTGGTgcggcgc                                                                                                      >  3:379781/1‑134 (MQ=255)                            ccAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGGACCGTCGCCCCGGATTGCCAGATCGCTTGGCGCCACACGCGCGGGGATGTCTGGTGCGGCGa                                                                                                      >  3:127432/1‑135 (MQ=37)                              aGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCACTGATTTCGCGGTCGCGTTGCGCCCCAGGCGCGGGGGTGTCTTGTgcgggggcc                                                                                                    >  5:46626/1‑130 (MQ=21)                                 cctggggtcCCAGCGGCGGCCAGCACCCGGCCGGGGCTGCGTGCTGCGGGGCGGCCGCGGACGACGGTACCGGCGCCACTGATTGCCAGCTCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTg                                                                                                  <  2:132866/129‑1 (MQ=255)                                 aCAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGGACCGTCGCCCCTGATTGCCAGATCGCTTGGCGCCCCAGGCGCGGGGCTGTCTGGTGCGGCGACCTGc                                                                                                 >  3:92142/1‑135 (MQ=37)                                 aCAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCCCAGATTGCCAGCTCGCTTGGCGCCCCAGGCGCGGGGGTGTGAGGTGCCGCGCCCGGc                                                                                                 >  2:422011/1‑135 (MQ=25)                                   aggggggCCAGCGGCGGCCAGCACACGGCCGGTGCTGCCTGCTGCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCAcc                                                                                              <  8:422111/130‑1 (MQ=255)                                   aGTGGTGCCATCGGCGGCCAGCACTCGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCAc                                                                                               >  6:331082/1‑135 (MQ=255)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTTCCGTCGCCACAGATTGCCAGATCGCTTGGCGCCACACGCGCGGTGGTGTCTGGTGCGGCGACCGGCCCCGGCa                                                                                          >  1:246381/1‑135 (MQ=37)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTTACCACGCCACAGAATTCCACATACCATGGCGCCACACGCGCGGTGAAGTAAGGTTCGGCGACCAGCACCCGcc                                                                                          >  1:183678/1‑134 (MQ=16)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGcc                                                                                          >  5:184705/1‑134 (MQ=255)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCaa                                                                                         >  7:426207/1‑136 (MQ=255)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATAGCATGGCGCCACACGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCa                                                                                          >  7:73782/1‑135 (MQ=255)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGGCGCCCCTGATTGCCAGATAGCTTGGCGCCACAGGCGCGGGGAGGGCTGGGGCGGCGcccggccccggcca                                                                                         >  7:188604/1‑134 (MQ=25)                                        tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGGCGCCCCGGCTTGCCAGCTCGCGTGGCGCCACCGGCGCGGGGCTGTCTGGTGCGGCGCCCGGCCCCGGCa                                                                                          >  3:392919/1‑135 (MQ=25)                                          ccATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGGCACTGCTTGCCAGATCGCTTGGCGCCCCAGGCGCGGGGGTGTCTGGTGCGGCGCCCTGCACCgggcac                                                                                        >  3:430794/1‑131 (MQ=37)                                          ccATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACCGTACCCTCGCCACTGATTGGCAGCTCGCTTTGCGCCCCCGGCGCGGGGCTGGCTGGTGCGGCGCCCGGCACCGGCAacg                                                                                       >  7:448004/1‑136 (MQ=25)                                           cagcggcggCAAGAACAGGGAGGGGGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAacg                                                                                       <  6:237617/132‑1 (MQ=255)                                           cATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAacga                                                                                      >  8:45744/1‑136 (MQ=255)                                           cATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACAGCTTTGCAGATCGCTTGGCGCCCCCGGCGCGGGGTTGTATGGGGCGGCGACCTgcaacggcaacg                                                                                       >  3:381209/1‑135 (MQ=37)                                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGCCGGTTCCGTCGCCACGGATTGCCCGCTCGCGTGGCGCCCCAGGCGCGGGGCTGTCTGGTGCGGCGGCCGGCACCCGCAccgaa                                                                                     >  3:346186/1‑134 (MQ=25)                                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGGCACTGAATGCCCGAACGCTTTGCGCCCCAGGCCCGGTTATGTCTGGTTCGGCGACCTGCCCCGGCCacgacg                                                                                    >  8:188322/1‑136 (MQ=25)                                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACCGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAacgac                                                                                     >  5:231046/1‑135 (MQ=255)                                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAacgacg                                                                                    <  8:426207/136‑1 (MQ=255)                                             tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACGGATTGCCAGATCGCTTGGCGCCCCAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAacgcc                                                                                     >  2:10506/1‑133 (MQ=255)                                                  ggcCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTg                                                                               <  8:244722/136‑1 (MQ=255)                                                           ccggccggGGCTGCCGGCGGCGGCGCGGCCGCGGACGACGGTACCGGCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGATGAGGTGCCgttgtt                                                                       <  5:2436/134‑1 (MQ=255)                                                             ggacggGGCTGCCTGCTGCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTc                                                                    <  8:270996/136‑1 (MQ=255)                                                              gacggTGCTGCCTGCTTCGGCGCGGCCGCTGACGACCGTACCGTCGCCACAGCTTGCCATATCGCTTGGCCCCACCAGCGCGGGGCTGTCTGGTGCCGCGGCCTGCCCCGGCAACCAAGCGGTGCCGGTGTTGTc                                                                    >  4:251606/1‑135 (MQ=255)                                                                cggTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCCCAGAGTGCCAGCTCGCTAGGCGCCCCAGGCGCGGGGCTGTCTGGTGCGGCGCCCGGCACCGGCCACGCCGCCGTGCCGTTGTTGGCgc                                                                  >  2:289009/1‑134 (MQ=255)                                                                 ggTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCCCTGATTGGCAGCTCGCGTGGCGCCCCAGGCGCGGGGGTGTGTGGTGCGGCGGCCTGCCCCGGCCACCCCCCGGTGCCGTTGTTGTCgct                                                                 >  6:340988/1‑133 (MQ=255)                                                                     ctgcctgcTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGGCAGATCGCTTTGCGCCCCAGGCGCGGGGATGTGTGGTGCGGCGACCTGCCCCCGCAACGCCGCGGTGCCCTTGTTGTCGATCGcc                                                             >  5:134744/1‑135 (MQ=255)                                                                                ggCGCGGCCGCTGACGACGGTACCGGCGCCCCTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGAGGTCTGGTTGCGCGACCGGCACCGGGCACGACGAGGTGCCGTTGTTGTCGATCGCCACCCCGTGCAg                                                  >  4:262424/1‑135 (MQ=255)                                                                                 gggcgGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCa                                                <  7:239812/134‑1 (MQ=255)                                                                                       cgtcgtacgacgGTACCGTCGCCTCTGATTGCCAGATCGCTTGGCGCCATCGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGAAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                           <  5:340692/129‑1 (MQ=255)                                                                                       cggCTGACGACGGTACCGTCGCCACTGATTGCCAGAGCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                           <  1:375448/133‑1 (MQ=255)                                                                                       ccGCGGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                           <  6:231189/135‑1 (MQ=255)                                                                                         gCTGACGACGGTTCCGTCGCCACGGATTGGCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGCCCTGCACCGGCAACCCCGAGGTGCCGGTGTTGTGGATCGCCACCCCGTCCAGCACCTCGc                                          >  4:350073/1‑134 (MQ=255)                                                                                                ccgGTACCGGCGCCACTGATTGCCAGCTCGCTTGGCGCCACAGGCGCGGTGATGTCTGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                 <  8:401193/135‑1 (MQ=255)                                                                                                 cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                 <  5:331082/135‑1 (MQ=255)                                                                                                 cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                <  8:18538/136‑1 (MQ=255)                                                                                                  ggTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                <  6:134744/135‑1 (MQ=255)                                                                                                  ggTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                <  2:16133/135‑1 (MQ=255)                                                                                                   gTACCGTCGCCACTGATTGCCTGATCGCTTGGCGCCCCAGGCGCGGTGATGGCTGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTgg                               <  3:4373/135‑1 (MQ=255)                                                                                                   gTACCGTCGCCACTGATTGCCAGATCGCTGGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTgg                               <  1:422011/135‑1 (MQ=255)                                                                                                   gTACCGGCGCCACTGATTGCCAGTTGGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTgg                               <  2:183678/135‑1 (MQ=255)                                                                                                    tACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGCCGAGGTGCCGTTGTTGTCGCTCGCCCCCACGTCCCGCCACTCGCCATTGGCCTggg                              >  6:409690/1‑135 (MQ=255)                                                                                                    tACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGACGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTggg                              >  6:189650/1‑135 (MQ=255)                                                                                                       cggcGCCAGTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCgg                          <  8:448004/133‑1 (MQ=255)                                                                                                       cGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGcc                            <  4:127432/134‑1 (MQ=255)                                                                                                        ggcGCCACTGATTGCCCGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCgg                          <  5:235121/133‑1 (MQ=255)                                                                                                        ggcGCCACTGATTGCCAGAGCGCTGGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCgg                          <  4:392919/133‑1 (MQ=255)                                                                                                            ccACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTTCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCCCCACCACGTCCAGCAACTCGCCATTGGCCTGGTCCGGTATaa                     >  7:247704/1‑136 (MQ=255)                                                                                                                tGATTGCCAGTTCTCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACGGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGca                  <  3:350073/135‑1 (MQ=255)                                                                                                                 gATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCAATGGCCTGGGCCGGTTTAAGcac                 >  2:143931/1‑135 (MQ=255)                                                                                                                   ttGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGcacca               <  2:90137/135‑1 (MQ=255)                                                                                                                    tGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAACcaccac              >  6:94372/1‑135 (MQ=255)                                                                                                                              ctgtgcgcCACAGGCGCGGGGATGGCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCAACACTTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTg    <  5:72491/131‑1 (MQ=255)                                                                                                                              cggggCGCCCCCGGCGCGGTGAGGGCTGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTg    <  6:25507/132‑1 (MQ=255)                                                                                                                               ttGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGcc  >  7:130579/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                CGCCGCTGGCCCCCACCACGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGCGGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCC  >  KT2440/930331‑930592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑