Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	967,684	C→T	10.8%	A9V (GCG→GTG)	CJ019_00912 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	967,684	0	C	T	10.8%	107.7 / 4.5	37	A9V (GCG→GTG)	CJ019_00912	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (5/28);  new base T (2/2);  total (7/30)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTTGGCGATGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCCGCGTGAAGTTT  >  KT2440/967553‑967818                                                                                                                                    |                                                                                                                                       ccGTTTGGCGATGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGggtgcggtg                                                                                                                                    <  8:71615/136‑1 (MQ=255) ccGTTTGGCGATGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGggtgcggt                                                                                                                                     <  5:232188/135‑1 (MQ=255)   gtgtGGCGATGAAACGGCGCGTGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCggtgct                                                                                                                                  <  7:170250/133‑1 (MQ=255)       ggCGATGAAAAGGCGCGAGTGGTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCggtgctgg                                                                                                                                <  6:420308/134‑1 (MQ=255)        gCGATGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGGGCggtgctgggt                                                                                                                              <  8:21231/135‑1 (MQ=255)          gATGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCggtgctgggtgc                                                                                                                            <  6:76347/135‑1 (MQ=255)           aTGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCggtgctgggtgct                                                                                                                           <  5:309755/135‑1 (MQ=255)                         gtgtTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGc                                                                                                             <  3:413171/135‑1 (MQ=255)                         gtgtTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGGGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGc                                                                                                             <  3:413175/135‑1 (MQ=255)                         gtggtgCACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGCAGCGCGGGTTCACACCCGCTGGGACGATCAGGAGGTTCACGTGAATAAATCAATGCGGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGc                                                                                                             <  1:176428/135‑1 (MQ=255)                           gtTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCg                                                                                                           <  6:209930/135‑1 (MQ=255)                              gAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAg                                                                                                        <  8:383360/135‑1 (MQ=255)                                    gcgcCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCtg                                                                                                  <  5:320199/135‑1 (MQ=255)                                     cgcCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCtg                                                                                                  <  4:324957/134‑1 (MQ=255)                                     cgcATTTTTTTTTGCTCATAGGCGGTGAGAAGAGCTGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTTAATAAATCTATGCTGGTGGGTGCGGTGCTGGGTGCTGGAGGTGTAACTGCAGGAGGTGCt                                                                                                   <  4:68744/133‑1 (MQ=255)                                        cTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTGCACACCCGCTGGTACGAGCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGc                                                                                              <  6:68907/135‑1 (MQ=255)                                            ttttttGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGAc                                                                                           <  3:212762/134‑1 (MQ=255)                                                ttGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTAc                                                                                       <  1:325044/134‑1 (MQ=255)                                                 tGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACa                                                                                      <  1:113376/134‑1 (MQ=255)                                                          gCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGaa                                                                            <  2:26603/135‑1 (MQ=255)                                                                         ggTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTc                                                            <  8:228082/136‑1 (MQ=255)                                                                          gTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTc                                                            <  1:99096/135‑1 (MQ=255)                                                                                                  gaggTTCACGTGAATAAATCAATGCTGGTGGGTGTGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGa                                   >  8:349614/1‑136 (MQ=255)                                                                                                   aggTTCAAGAGAATAAATCAAGGCGGGTGGGGGCGGTGCTGGGTGCGGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAACGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGa                                   <  5:260636/135‑1 (MQ=255)                                                                                                        cACGTGAATAAATCAATGCTGGTGGGTGTGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAag                              <  7:349614/135‑1 (MQ=255)                                                                                                         aCGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAag                              <  3:23563/134‑1 (MQ=255)                                                                                                         aCGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAag                              <  2:414445/134‑1 (MQ=255)                                                                                                          cGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAg                                                                                     <  2:357086/78‑1 (MQ=255)                                                                                                          cGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAg                                                                                     >  1:357086/1‑78 (MQ=255)                                                                                                           gTGAATAAATCAATGCTGGTGGGTGTGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAagca                            >  5:178501/1‑134 (MQ=255)                                                                                                           gTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGc                                                                                                             >  4:54624/1‑53 (MQ=255)                                                                                                           gTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGc                                                                                                             <  3:54624/53‑1 (MQ=255)                                                                                                           gTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAagca                            >  6:75545/1‑134 (MQ=255)                                                                                                                  aaTCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGATGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGaa                   <  7:333837/136‑1 (MQ=255)                                                                                                                     cAATGCTGGTGGGTGTGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGaaaa                 <  6:178501/135‑1 (MQ=255)                                                                                                                           tggtggGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGCCGTGCAGCCGATCAAGCAGCAGGTGAAAACCCcgc           >  6:291551/1‑135 (MQ=255)                                                                                                                           tggtggGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCcgc           >  5:213396/1‑135 (MQ=255)                                                                                                                             gtgggtgCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCCGCGt         <  8:55761/135‑1 (MQ=255)                                                                                                                              tgggtgCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCCGCGt         <  6:213396/134‑1 (MQ=255)                                                                                                                                ggtgcggtgcTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAATCAGCAGGTGAAAACCCCGCGTGAAg     >  8:399230/1‑136 (MQ=255)                                                                                                                                ggtgcggtgcTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCCGCGTGaa      >  3:399963/1‑135 (MQ=255)                                                                                                                                    cggtgcTGGGTGCTGTCGTTGTAACTGCCGGAGGTGCTGGGCCGACCTACAGCTTGGTGAACAAAGGCCCCCAAGTCGCCCAGTGGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCCGCGTGAAGttt  <  2:396884/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       CCGTTTGGCGATGAAAAGGCGCGAGTGTTGAACTCGCGCCTTTTTTTTTGCTCCAAGGCTGGGAGAAGCGCGGGTTCACACCCGCTGGTACGATCAGGAGGTTCACGTGAATAAATCAATGCTGGTGGGTGCGGTGCTGGGTGCTGTCGGTGTAACTGCCGGAGGTGCTGTGGCGACCTACAGCTTGGTGAACAAAGGCCCCGAAGTCGCCCAGGTGACTGACGTGCAGCCGATCAAGCAGCAGGTGAAAACCCCGCGTGAAGTTT  >  KT2440/967553‑967818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑