Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 996,842 C→A 5.6% A100D (GCC→GAC)  fecI_5 → putative RNA polymerase sigma factor FecI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440996,8420CA 5.6% 199.8 / 2.3 72A100D (GCC→GAC) fecI_5putative RNA polymerase sigma factor FecI
Reads supporting (aligned to +/- strand):  ref base C (30/38);  new base A (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

ATCAGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCGAAAAG  >  KT2440/996707‑996975
                                                                                                                                       |                                                                                                                                     
aTCAGGCTGCGCGCAATATCGCTGGGGAGGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       <  8:40849/136‑1 (MQ=255)
 tCAGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       <  4:348057/135‑1 (MQ=255)
 tCAGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       <  5:171435/135‑1 (MQ=255)
 tCAGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       <  3:149450/135‑1 (MQ=255)
 gcAGGCTGCGCGCAATATCGCGGGGGAGGGCTTCCGGCGCGAAGACCGGCGCCAGGCCCGGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       <  2:353025/134‑1 (MQ=255)
   aGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCg                                                                                                                                     >  6:407387/1‑135 (MQ=255)
   aGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCg                                                                                                                                     >  2:167293/1‑135 (MQ=255)
       tGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCCTGCCCTGGAGCTGGCCGACAg                                                                                                                                 >  3:272872/1‑135 (MQ=255)
       tGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAg                                                                                                                                 >  2:75628/1‑135 (MQ=255)
       tGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAg                                                                                                                                 >  6:184675/1‑135 (MQ=255)
       tGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGACGACAg                                                                                                                                 >  1:259352/1‑135 (MQ=255)
       tGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGACGACAg                                                                                                                                 >  1:252089/1‑135 (MQ=255)
          gcgcAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGt                                                                                                                              >  6:77932/1‑135 (MQ=255)
            gcAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGCCAGCGt                                                                                                                              >  1:267597/1‑133 (MQ=255)
            gcAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGt                                                                                                                              >  1:46939/1‑133 (MQ=255)
                        tgGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTgg                                                                                                                >  3:37344/1‑135 (MQ=255)
                           aTGGCTTCCGTCGCGAAGACCGGCGCCCGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCg                                                                                                             <  6:122614/135‑1 (MQ=255)
                           aTGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGCCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGCGCGGGCCCTGGcc                                                                                                              >  8:89603/1‑134 (MQ=255)
                             ggCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAg                                                                                                           >  4:168180/1‑135 (MQ=255)
                             ggCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCCTTGAGCGGGCCCTGGCCGAg                                                                                                           >  6:61530/1‑135 (MQ=255)
                                ttCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACa                                                                                                                                  >  5:308128/1‑109 (MQ=255)
                                ttCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACa                                                                                                                                  <  6:308128/109‑1 (MQ=255)
                                   cGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCct                                                                                                     >  6:442554/1‑135 (MQ=255)
                                     tCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCctct                                                                                                   <  4:418015/135‑1 (MQ=255)
                                     ccgcgAAGACCGGCGCCTGGCCCTGGAGCGGGAAGCCTTCGAAGACGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCctct                                                                                                   <  3:353756/134‑1 (MQ=255)
                                        cgAAGCCCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAAc                                                                                               >  8:148787/1‑136 (MQ=255)
                                             aCCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGcc                                                                                           >  1:215550/1‑135 (MQ=255)
                                                   gcCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGgt                                                                                    >  8:306703/1‑136 (MQ=255)
                                                    ccAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGgt                                                                                    >  3:30466/1‑135 (MQ=255)
                                                     cAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGCGCTGCCGCTCAACTGCCGGCAGgtg                                                                                   >  2:422649/1‑135 (MQ=255)
                                                          ccTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGCCAGCGTTGAGCGGGCCCTGGCCGAGCTGCCGCTCAACTGCCGGCAGGTGTTctt                                                                              >  5:327069/1‑133 (MQ=255)
                                                          ccTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATc                                                                             >  7:139653/1‑136 (MQ=255)
                                                          ccTGGAGCCGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCAGGAGCTTGCCGACAGCGTTGAGCGGGCCCAGGCCGAGCTGCCTCTCAACTGCCCGCAGGTGTTCAt                                                                              >  4:44826/1‑135 (MQ=255)
                                                              gctgtggATGCCTTCTAACATGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCGCTGGAGCTGGCCGAGAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGc                                                                         <  7:168403/131‑1 (MQ=255)
                                                              gAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTgg                                                                          <  7:254007/135‑1 (MQ=255)
                                                               aGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGATGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTACATCTg                                                                           >  5:146533/1‑133 (MQ=255)
                                                                     acggcTTCGACTACGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGGTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGc                                                                   <  1:21183/131‑1 (MQ=255)
                                                                             gaagaaGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAgg                                                            <  4:133384/134‑1 (MQ=255)
                                                                                tcgggCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTg                                                        <  1:170077/132‑1 (MQ=255)
                                                                                gaagGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTg                                                        <  5:407387/135‑1 (MQ=255)
                                                                                     cgcAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCa                                                   >  5:76262/1‑135 (MQ=255)
                                                                                     cgcAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAg                                                  >  7:21185/1‑136 (MQ=255)
                                                                                     cgaaaCCGGCAGTGGCGACCCGGAAGCCTAAGTGCAGGCCCTGGCGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCa                                                   <  6:78083/132‑1 (MQ=255)
                                                                                      gcAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAg                                                  >  4:3990/1‑135 (MQ=255)
                                                                                                gTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       <  3:373140/40‑1 (MQ=255)
                                                                                                gTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGc                                                                                                                                       >  4:373140/1‑40 (MQ=255)
                                                                                                 tGGCGACCCGGCAGCCGTCGTGCAGGCCCGGGAGCTGGCCGACAGCGGTGAGCGGGCCCGGGCCGAGCGGACTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGc                                       <  2:184581/135‑1 (MQ=255)
                                                                                                 tGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGc                                       <  6:196213/135‑1 (MQ=255)
                                                                                                 tGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGc                                       <  6:169991/135‑1 (MQ=255)
                                                                                                 gggCGACCCGGACGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGGTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGc                                       <  2:184584/134‑1 (MQ=255)
                                                                                                 gggCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGc                                       <  5:257021/134‑1 (MQ=255)
                                                                                                      aCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAgc                                  <  6:76262/135‑1 (MQ=255)
                                                                                                      aCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAgc                                  <  2:46939/135‑1 (MQ=255)
                                                                                                      aCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAgc                                  <  3:239247/135‑1 (MQ=255)
                                                                                                      aCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCGCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAgc                                  <  1:291751/135‑1 (MQ=255)
                                                                                                      aCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGACGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAgc                                  <  2:259352/135‑1 (MQ=255)
                                                                                                      aCCCGGAAGCCTACGTGCATGCCCTGGAGCTGGACGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAgc                                  <  2:252089/135‑1 (MQ=255)
                                                                                                          ggAAGCCTACGTGCAGGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATg                             <  8:208439/136‑1 (MQ=255)
                                                                                                           gAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATgg                            <  8:304247/136‑1 (MQ=255)
                                                                                                            aaGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATgg                            <  5:13714/135‑1 (MQ=255)
                                                                                                             aGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATggg                           <  4:30466/135‑1 (MQ=255)
                                                                                                               ccTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGtt                        <  7:308140/136‑1 (MQ=255)
                                                                                                                    gTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGc                    <  3:41993/135‑1 (MQ=255)
                                                                                                                      gCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGc                    >  7:199714/1‑133 (MQ=255)
                                                                                                                        aTGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGa                <  5:129513/135‑1 (MQ=255)
                                                                                                                            ccTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACAt            >  4:407242/1‑135 (MQ=255)
                                                                                                                            ccTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACAt            >  4:279057/1‑135 (MQ=255)
                                                                                                                            ccTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATg           >  8:362399/1‑136 (MQ=255)
                                                                                                                              tGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATgg          <  3:3990/135‑1 (MQ=255)
                                                                                                                                gAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTc        <  7:358099/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  3:279057/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  5:77932/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  3:443353/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  1:75628/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  5:184675/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  1:290418/135‑1 (MQ=255)
                                                                                                                                 aGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCg       <  1:160700/135‑1 (MQ=255)
                                                                                                                                  gCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGTCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGGCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCGa      <  4:272872/135‑1 (MQ=255)
                                                                                                                                      gCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCGAAAAg  <  3:220141/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
ATCAGGCTGCGCGCAATATCGCTGTGGATGGCTTCCGTCGCGAAGACCGGCGCCAGGCCCTGGAGCGGGAAGCCTTCGAAGAAGGCGCAACCGGCAGTGGCGACCCGGAAGCCTACGTGCATGCCCTGGAGCTGGCCGACAGCGTTGAGCGGGCCCTGGCCGAGCTGCCTCTCAACTGCCGGCAGGTGTTCATCTGGCAGAAGCTCGAAGGCCTGACCCAGGCCGAGATTGCCGAGCGCATGGGGTTGAGCAAGAACATGGTCGAAAAG  >  KT2440/996707‑996975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑