Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,005,066 A→C 32.2% D143A (GAC→GCC)  prfC → Peptide chain release factor RF3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,005,0660AC32.2% 30.4 / 3.0 40D143A (GAC→GCC) prfCPeptide chain release factor RF3
Reads supporting (aligned to +/- strand):  ref base A (15/11);  new base C (9/5);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCATCGCCCTGATGGACGTGTGCCGCCTGCGCGACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACTACAT  >  KT2440/1004945‑1005201
                                                                                                                         |                                                                                                                                       
gcgGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCCTCGCCCTGATGGACGTGTGCCGCCTGCGCGCCCCGCCCATCGTACGCTTCATAACCCAACTCGACCGCGCCCTCcgc                                                                                                                            >  3:330950/1‑135 (MQ=255)
gcgGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCATCGCCCTGATGGACGTGTGCCGCCAGCGCGCCACGCCCATCGTCAGCTTCCTCCACAAACTCGACCGAGACCTCcgc                                                                                                                            >  3:223021/1‑135 (MQ=255)
          cGGCGCTGATGGTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCATCGCCCTGATGGCCGTGTGGCGCCTGCGCGACCCGCCCCTCGTCCGGGTGATCAACCAACACGCCCGCGCCAACCGCGCCCCGCTc                                                                                                                   >  3:378865/1‑134 (MQ=255)
              gcTGATGGTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCATCGCCCTGATGGGCGTGTGCCGCCTGCGCGCCACGCCCCTCGTCCGCTTCATCAACAAACTCGCCCGGGCCATACGCGCCCCGATCGCACt                                                                                                              >  3:204741/1‑135 (MQ=255)
                     gTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCATCGCCCAGATGGACCTGTGCCGCCTTCGCGCCACGCCCAACGTCAGCATCATAAACAAACTCGCCCCACCCCACCGCGACCCGAAAGAACGACTcgcc                                                                                                       >  4:226269/1‑133 (MQ=255)
                                    aaaGGGGTAGAGCCGCGTACCAGCGCCCTGATGGACGTGTGCCGCCGGCGCGACACGCCAATCGTCAGCTTCAACAAAAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGtt                                                                                        <  3:152406/135‑1 (MQ=255)
                                              agCCGCTTACCATCGCCCTGATGGCCGTGTGCCGCCTGCGCGACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCa                                                                              <  3:405182/135‑1 (MQ=255)
                                                ccGCGTACCATCGCCCTGATGGCCGTGTGCCGCCTGCGCGCCACGCCCATCCGCAGCTTCATCCACAAACACGCCCGGGCCAACCCCGCCCCGATCGCACAGCGCGGCCAGAACGAAGCCGGTTTGAAGATCAAg                                                                            >  2:281454/1‑135 (MQ=255)
                                                   cgTACCATCGCCCTGATGGACGTGTGCCGCCTGCGCGCCACGCCCATCGTCAGCGTCATCAACCAAACCGCCCGAGACCACCGCGACCCGCTCGAACTGCTCGCCGCGATCGAAGCCGTTTTGAATAGCAAGGCcg                                                                        >  8:33841/1‑136 (MQ=255)
                                                      aCCATCGCCCTGATGGACGTGTGCCGCCTGCGCGACACGCCCATAGTCAGCTTCATCAACAAACTAGACCGCGACATCCGCGACCCGATCGAAATGCTCGACGCGATCGCAGCCGGTTTGAAGATCAAGGCcgcg                                                                      >  6:173180/1‑135 (MQ=255)
                                                      aCCATCGCCCTGATGGACGTGTGCCGCCGGCGCGCCCCGCCCATCGTCACCGTCATCAACAAACACGCCCGAGACATCCCCCACCCGATAGCACTGCTCGACGAGCTCGCAGCCGTTTTGAATAACAAGGCcgcg                                                                      >  2:362043/1‑135 (MQ=255)
                                                      aCCATCGCCCTGATGGACGTGTGCCGCCAGCGCGACACGCCCATCGGCAGCGTCATCAACAAACTCGACCGAGCCATCCCCGCCCCGATCGCACTGCTCGACGCGCTCGAAGCCGGTTTGAAGATCAAGGCCgcgc                                                                     >  8:449223/1‑136 (MQ=255)
                                                             cccTGATGGACGTGTGCCGCCTGCGCGCCACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGCCCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCa                                                               >  2:13080/1‑135 (MQ=255)
                                                             cccTGATGGACGTGTGCCGCCTGCGCGACACGCCCATCGTCCGCTACCTCAACACACTCGACCGAGACATCCGCCACCCGATCCAACTGCTCGACCCCCAGCAAGCCCTGTTGAAGATCAACGCCGCGCCAATCa                                                               >  2:343214/1‑135 (MQ=255)
                                                                tGATGGACGTGTGCCGCCTGCGCCCAACGCCCCACGTCAGCGTCCTCCACAAACGCGCCCGCGACATCCGCGACCCGCTCGCACTGCTCGACGAGATCGAACCCCGTTTGAAGCTAAAGGCCGCCCCAATccact                                                            >  4:62343/1‑131 (MQ=255)
                                                                     gACGTGTGCCGCCTGCGCGACACGCCCCTCGTCAGCGTCATCAACAAAATCGCCCGAGCCCTCCGCGCCCCGCTCGAAAAGCTCGACGAGCTCGACGCCGTTTTGAAGGTCAATGCCGCGCCCCTACCCTGGCCg                                                       >  2:193911/1‑135 (MQ=255)
                                                                     gACGTGTGCCGCCTGCGCGACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCg                                                       >  3:336094/1‑135 (MQ=255)
                                                                           tgCCGCCTGCGCGACACGCCCATCGTCCGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGCAGCCGTTTTGAAGATCAAGGCCGCGCCAATCCCCTGGCCGCTCGGt                                                 >  1:274336/1‑135 (MQ=255)
                                                                           tgCCGCCTGCGCGACACGCCCATCGTCAGCTTCATCAACAAACTCGCCCGAGACATCCGCGCCCCGATCGAACTGCTCGACGAGATAGCAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGt                                                 >  4:157071/1‑135 (MQ=255)
                                                                              cgccTGCGCGACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTg                                               >  6:149595/1‑134 (MQ=255)
                                                                                ccTGCGCGACACGCCCCTCGTCAGCTTAATCAACAACCTCGACCGCGACAACCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTAc                                           <  7:125961/136‑1 (MQ=255)
                                                                                 cggcgcGACCCGCCCATCGTCAGCTACAACAACAAACTCCCCCGACACACCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTAc                                           <  5:235927/133‑1 (MQ=255)
                                                                                   gcgcGACACGCCCATCGTCCGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGCAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTTCcgc                                        >  8:29831/1‑136 (MQ=255)
                                                                                   gcgcGACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACcgc                                        >  7:209716/1‑136 (MQ=255)
                                                                                       gACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGAc                                     >  3:70444/1‑135 (MQ=255)
                                                                                        acagGCCCATCGTCAGCTTCATCAAAAACCTCGCCCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCTGTTGCTACCGCGACt                                    <  6:147987/135‑1 (MQ=255)
                                                                                        acacGCCCATCGTCAGATTCATCAACAAACTCGACCGAGAAATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACtt                                   <  7:33841/136‑1 (MQ=255)
                                                                                           cGCCCATCGTCAGCTCAACCAAAAACCTCGCCCGACACAACCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCa                                 <  1:140399/135‑1 (MQ=255)
                                                                                               cATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGc                                                         <  2:377787/107‑1 (MQ=255)
                                                                                               cATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGc                                                         >  1:377787/1‑107 (MQ=255)
                                                                                                      agattCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTacc                     <  8:64491/133‑1 (MQ=255)
                                                                                                           catcaACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTaccacc                  >  5:320596/1‑134 (MQ=255)
                                                                                                            atcaACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTaccacc                  <  4:378865/133‑1 (MQ=255)
                                                                                                               aacaaACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCg            >  7:298407/1‑136 (MQ=255)
                                                                                                                acaaACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGAcc             <  4:196871/134‑1 (MQ=255)
                                                                                                                aaaaaCTCTACCGACACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCgg           <  7:221154/134‑1 (MQ=255)
                                                                                                                 caacatCGACCTAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCgg           <  4:22465/130‑1 (MQ=255)
                                                                                                                   aaCTCCCCCGAGACAGCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGGCGTTTTGAAGAGCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTg         <  4:142581/135‑1 (MQ=255)
                                                                                                                      tCGCCCGCGAGACCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACt      <  1:343214/135‑1 (MQ=255)
                                                                                                                      tCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACt      <  5:149595/135‑1 (MQ=255)
                                                                                                                        caCCGAGACAGCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACTAc    <  2:308909/134‑1 (MQ=255)
                                                                                                                         aCCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACTACa   <  6:320596/135‑1 (MQ=255)
                                                                                                                         aCCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACTACAt  >  7:260339/1‑136 (MQ=255)
                                                                                                                         |                                                                                                                                       
GCGGTGGACTCGGCGCTGATGGTGCTCGACGGCGGTAAAGGTGTAGAGCCGCGTACCATCGCCCTGATGGACGTGTGCCGCCTGCGCGACACGCCCATCGTCAGCTTCATCAACAAACTCGACCGAGACATCCGCGACCCGATCGAACTGCTCGACGAGATCGAAGCCGTTTTGAAGATCAAGGCCGCGCCAATCACCTGGCCGATCGGTTGCTACCGCGACTTCAAGGGCGTGTACCACCTGACCGGTGACTACAT  >  KT2440/1004945‑1005201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑