Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,138,117	C→T	5.7%	P90P (CCG→CCA)	pigA_1 ←	Heme oxygenase PigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,138,117	0	C	T	5.7%	214.3 / 3.1	70	P90P (CCG→CCA)	pigA_1	Heme oxygenase PigA
Reads supporting (aligned to +/- strand):  ref base C (24/42);  new base T (2/2);  total (26/44)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

AAGCTGTCGGACAGTTCCAGGGCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCGGACTGGAATAGGTACT  >  KT2440/1137983‑1138250                                                                                                                                       |                                                                                                                                      aaGCTGTCGGACAGTTCCAGGGCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCg                                                                                                                                      >  8:317896/1‑136 (MQ=255) aaGCTGTCGGACAGTTCCAGGGCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCg                                                                                                                                      >  8:311781/1‑136 (MQ=255)                tCCAGGGCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGGCg                                                                                                                        <  4:168606/135‑1 (MQ=255)                  cAGGGCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAg                                                                                                                      >  1:242921/1‑135 (MQ=255)                     ggCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTc                                                                                                                   <  5:194123/135‑1 (MQ=255)                     ggCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTc                                                                                                                   <  3:72269/135‑1 (MQ=255)                     ggCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTc                                                                                                                   <  5:49582/135‑1 (MQ=255)                       ccACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCgg                                                                                                                 >  5:107552/1‑135 (MQ=255)                            gCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAgg                                                                                                            >  6:438947/1‑135 (MQ=255)                            gCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAgg                                                                                                            >  6:74883/1‑135 (MQ=255)                            gCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAgg                                                                                                            >  3:374442/1‑135 (MQ=255)                            gCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGt                                                                                                           >  8:201039/1‑136 (MQ=255)                            gCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCACATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTGCGGTGTCGAGGTCGGCAAgg                                                                                                            >  6:138685/1‑135 (MQ=255)                                  ttGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGc                                                                                                     >  7:256472/1‑136 (MQ=255)                                   tGATCAGAAACGCCGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCg                                                                                                    <  8:259392/136‑1 (MQ=255)                                    gATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGATACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCg                                                                                                    <  8:252927/135‑1 (MQ=255)                                     aTCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCg                                                                                                    <  6:238716/134‑1 (MQ=255)                                        aGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCTGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGcc                                                                                                >  3:226852/1‑135 (MQ=255)                                        aGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGcc                                                                                                >  6:215815/1‑135 (MQ=255)                                        aGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATAGCCTCGCCCAGGCTCGGCTTGCACACGGCACCCCGTACCGCGGCCGGTCCTGCGGTGTCGAGGTCGGCAAGGTCAATGCGGGcc                                                                                                >  3:306099/1‑135 (MQ=255)                                         gAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCt                                                                                               <  5:215815/135‑1 (MQ=255)                                                  cgcCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGGCGAGGTCGGCAAGGGCAAGGCGGGCCTGCTcggcac                                                                                      <  5:220196/135‑1 (MQ=255)                                                       aGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTc                                                                                                                         >  7:238638/1‑95 (MQ=255)                                                       aGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTc                                                                                                                         <  8:238638/95‑1 (MQ=255)                                                       aGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAg                                                                                 >  5:181655/1‑135 (MQ=255)                                                             gAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTc                                                                            <  3:174011/134‑1 (MQ=255)                                                             gAGCCTTCGGAGACGAAGAGCCAGCCCATTGCCTCGCCCAGGCGCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTc                                                                            <  2:423753/134‑1 (MQ=255)                                                                  ttCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAg                                                                      <  6:181655/135‑1 (MQ=255)                                                                      gagaCGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCa                                                                  >  5:42542/1‑135 (MQ=255)                                                                      gagaCGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGGCGAGGTCGGCCACGTCAAGGCGGGCCTGCGCGACCCGGCCGCGCTCAGCCAGCTCa                                                                  >  5:286575/1‑135 (MQ=255)                                                                      gagaCGAAGATCCAGCCCATTGCCTCGCCCAGGCGCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATc                                                                   <  1:283028/134‑1 (MQ=255)                                                                               aTCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGAtt                                                         >  7:372588/1‑135 (MQ=255)                                                                                tCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTg                                                        >  2:15373/1‑135 (MQ=255)                                                                                 ccAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGc                                                       <  3:430247/135‑1 (MQ=255)                                                                                   aGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGGCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCg                                                      <  5:74883/134‑1 (MQ=255)                                                                                        aTTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCt                                               <  7:266586/136‑1 (MQ=255)                                                                                           gCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGa                                             <  8:235682/135‑1 (MQ=255)                                                                                            ccTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAgg                                           >  8:285726/1‑136 (MQ=255)                                                                                             cTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  8:379681/136‑1 (MQ=255)                                                                                             cTCGCCCAGGCTCGGCTTGCCCACGGCACCAGGTACCGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGAGGGCCTCCTCCGCACGGCAGCGCGCAGCCAGATCAGGGCCGATTGCGATCAGCTGAgg                                           >  5:291630/1‑135 (MQ=255)                                                                                              tCGCCGCGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGGCGGTACTTCGGGGTCGCGGTCGGCAAGGTCCAGGCGGGCCTGCTCGTCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  4:338301/135‑1 (MQ=255)                                                                                              tCGCCCAGGCTCGGCTTGCCCACGGCACCCTGAACGGCGGCCGGTACTTCGGTGTCGAGGGCGGCAAGGTCAAGGCGGGGCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  6:76515/135‑1 (MQ=255)                                                                                              tCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  5:438947/135‑1 (MQ=255)                                                                                              tCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  6:42542/135‑1 (MQ=255)                                                                                              tCGCCCAGGCTCGGCGTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGGCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  6:121710/135‑1 (MQ=255)                                                                                                 cccAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          >  5:247140/1‑132 (MQ=255)                                                                                                 cccAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAggg                                          <  6:247140/132‑1 (MQ=255)                                                                                                   cAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTg                                    <  7:285726/136‑1 (MQ=255)                                                                                                      gCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTAc                                 <  8:243938/136‑1 (MQ=255)                                                                                                         cGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCCCGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAgg                              >  8:363262/1‑136 (MQ=255)                                                                                                         cGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGCTCAGCTGAGGGTCGTTg                                    >  4:125216/1‑130 (MQ=255)                                                                                                         cGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTg                                    <  3:125216/130‑1 (MQ=255)                                                                                                                cccACGGCACCCGGTACGGCGGCCGGTACTTCTGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCa                       <  7:201039/136‑1 (MQ=255)                                                                                                                cccACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGc                        <  1:199190/135‑1 (MQ=255)                                                                                                                 ccACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCa                       <  6:107552/135‑1 (MQ=255)                                                                                                                 ccACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGGCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCa                       <  1:1025/135‑1 (MQ=255)                                                                                                                 ccACGGCACCCGGGACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCa                       <  6:52467/135‑1 (MQ=255)                                                                                                                   aCGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGCTTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCt                    >  7:205545/1‑136 (MQ=255)                                                                                                                   aCGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGc                     <  4:38863/135‑1 (MQ=255)                                                                                                                    cGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTc                   <  7:302490/136‑1 (MQ=255)                                                                                                                     ggCACGCGGTACGGCGGCCGGTACTTCGGGGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTc                   <  2:77850/135‑1 (MQ=255)                                                                                                                     ggCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGTGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTc                   <  3:323389/135‑1 (MQ=255)                                                                                                                       cACCCGGTACGGCGGCTGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCgg                 <  4:226852/135‑1 (MQ=255)                                                                                                                       cACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGTTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCgg                 <  5:150108/135‑1 (MQ=255)                                                                                                                       cACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCgg                 <  4:296933/135‑1 (MQ=255)                                                                                                                       cACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGGCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCgg                 <  4:291655/135‑1 (MQ=255)                                                                                                                           cGGTACGGCGGCCTGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGTGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCGGACTg             <  2:242921/135‑1 (MQ=255)                                                                                                                            ggTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCGGACTgg            <  6:80744/135‑1 (MQ=255)                                                                                                                               aCGGCGGCTGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGc                                                       <  4:166444/89‑1 (MQ=255)                                                                                                                               aCGGCGGCTGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGc                                                       >  3:166444/1‑89 (MQ=255)                                                                                                                                      ccGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCGGACTGGAATAGGTACt  >  5:348232/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                      AAGCTGTCGGACAGTTCCAGGGCCACGGCACGCTTGATCAGAAACGCAGCGCCCAGTTTGGAGCCTTCGGAGACGAAGATCCAGCCCATTGCCTCGCCCAGGCTCGGCTTGCCCACGGCACCCGGTACGGCGGCCGGTACTTCGGTGTCGAGGTCGGCAAGGTCAAGGCGGGCCTGCTCGGCACGGCAGCGCTCAGCCAGATCAGGGACGATTGCGATCAGCTGAGGGTCGTTGTACAGGGCCTGCAGCTCGGACTGGAATAGGTACT  >  KT2440/1137983‑1138250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑