| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,188,687 | A→C | 60.3% | S41R (AGC→CGC) | CJ019_01117 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,188,687 | 0 | A | C | 60.3% | ‑5.9 / 3.2 | 18 | S41R (AGC→CGC) | CJ019_01117 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base C (4/7); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
CCTCGTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGG‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTG > KT2440/1188560‑1188815 | ccgcGTGAGGCGCCCCTCGCGTCTGCGCACCGGGGCTGGCCTGCCCCGGGCCGGCGGGCTTTCTGCGCTGTGTGTGCGGCAGTGGCGCCGGCACCCACACCCGG‑CCAC‑GGCGCCTGCGGG‑GGCCGACAGCCCGCt < 6:364440/132‑1 (MQ=255) gATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACAGCccccggccccgg > 7:271173/1‑136 (MQ=255) aCCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCCGCCCCACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACCCCCCCCCGCCCcggggggtg > 1:136619/1‑129 (MQ=255) tCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACCGCCCCCGGCCCGGGGGGCtgggtggtggc > 7:440788/1‑133 (MQ=255) tGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACAGCCCCCGGCCCGGGCGGATGGGTGGTCACTGG‑CCGG‑ggccg > 7:357400/1‑136 (MQ=255) gCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACCGCCCCCGGCCCCGGCGGATGGGTGGGCACAGGCCCGG‑CCCCGccc > 5:313690/1‑135 (MQ=255) ccGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACCGCCCCCGGGCCGGGGGGTTGGGTTGTCACTGG‑CAGGCCCCCGCCCG‑CGGCGGCGcca > 5:307426/1‑135 (MQ=255) cgtggcgccggccgCCACACCGGG‑CCAC‑GCCGCGGGGGGG‑GGCCCACAGCCCGCGGCCGGGGCTGATGGTTGGGCAGTGG‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCt < 7:444167/130‑1 (MQ=255) ttggCCGGCCTCCACACCCGGCCCAG‑GGCGGCTGGGGG‑GCCCGACCGCCCGCTGCCCGGGGTTATGGTTGGGCAGTGC‑CCGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTc < 8:280226/132‑1 (MQ=255) ccACCCCCGG‑CCCC‑GGGGGCGGGGGGGGGCCCACCGCCCGCGGCCGGGGGTTTTGGTGGGGCACTGG‑CCGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAg < 7:59817/136‑1 (MQ=255) ccacccccG‑CCACGGGCGCGGGCGGG‑GCCCGACCCCCGGCTGCCCGGGGTTTTGGTTGGTCACTGG‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAg < 3:223093/131‑1 (MQ=255) cccgg‑cccc‑ggcGCCGGCGGG‑GCCCGACAGCCCGCGGCCCGGGCTGATGGTGGGCCACTGG‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAg < 8:352147/136‑1 (MQ=255) ggCGCGGGCGGG‑GGCGGACCGCCCGCTCCCCGGGATTTTGGTGGGCCCCGGG‑CCGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGgc < 4:408521/135‑1 (MQ=255) cgcCGGCGGG‑GGCCGACAGCCCGCGGCCCGGGCTGATGGTGGGGCACTGG‑CCGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGgccgc < 7:135293/136‑1 (MQ=255) gCCGACCGCCGGCGGCCGGGGGTTATGGTTGGTCCCTGG‑CCGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 6:358219/135‑1 (MQ=255) gCCGACCGCCCGCGGCCCGGGCGGAGGGTGGGCCACTGC‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 6:248151/135‑1 (MQ=255) ccggACCGCCCGCTGCCCGGGCTGATGGTTGGTCACTGC‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 6:313690/132‑1 (MQ=255) ccggACAGCCCGGGCCCCGGGGTGTTGGTTGGTCACTGG‑CGGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTgtggtg < 3:420466/132‑1 (MQ=255) | CCTCGTGATGCTCACCTCGCGTCTGCTCACCGGTGCTGGCCTGACCCTGGCCGGCTGGCTTTCTGCGCAGTGTGTGCTGCAGTTGAGCCGGCAGCCACACCCGG‑CCAC‑GGCGCCTGCGGT‑GGCCGACAGCCCGCTGCCCGGGCTGATGGTTGGTCACTGG‑CAGG‑CGCCGCCCGACGACGGCGCCATCACCATCACTCGTCTGCCGCTGCAATACCTCGGCGGCCTCAAGGCCCAGCCATTGGCCGCCAGTGTGGTG > KT2440/1188560‑1188815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |