Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,226,581 G→T 9.3% H53N (CAC→AAC)  glpR ← Glycerol‑3‑phosphate regulon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,226,5810GT 9.3% 126.5 / 2.0 50H53N (CAC→AAC) glpRGlycerol‑3‑phosphate regulon repressor
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base T (3/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGAACAGCGACGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGTTCGAGGAT  >  KT2440/1226447‑1226710
                                                                                                                                      |                                                                                                                                 
gagcaaCAGCGACGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGCCGGCACGCAGGGCGTAGGCGGGGTTCTCGATGCTCGAGTCTTAAGCTGCGCCGCCGTgg                                                                                                                                  <  7:434378/132‑1 (MQ=255)
   gaACAGCGACGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGGGGTa                                                                                                                                >  5:419436/1‑135 (MQ=255)
        gCGACGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGgc                                                                                                                           <  4:113996/135‑1 (MQ=255)
            cGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCgcag                                                                                                                       >  4:309023/1‑135 (MQ=255)
            cGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCgca                                                                                                                        >  6:319106/1‑134 (MQ=255)
                 ggTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGGCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGtt                                                                                                                 <  7:205012/136‑1 (MQ=255)
                      ggAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGc                                                                                                             >  1:57629/1‑135 (MQ=255)
                        aaTCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTc                                                                                                           <  5:98125/135‑1 (MQ=255)
                                         cGTCGGCAATGGGCTGCTTTTCGTCACGCAGCTGGTAGGCAGGCATGGCGTTGGCGGTGTTCTGGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTc                                                                                         <  8:169752/136‑1 (MQ=255)
                                           tCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTcg                                                                                        >  3:186999/1‑135 (MQ=255)
                                             ggCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGt                                                                                     <  8:27928/136‑1 (MQ=255)
                                             ggCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGt                                                                                     <  8:51955/136‑1 (MQ=255)
                                             ggCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGGGTTCTCGATGCGCGAGTCGTACGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGt                                                                                     <  8:16461/136‑1 (MQ=255)
                                              gCATTGCGCTGCTTTTCTTCACGCATCTGTTCGGCACTCATGGAGTAGGCTGTGTTCTCTATGCTCGATTATTAAGCTGAGCCGCAGTTGTAACTTAGCATAAGGTTGAGCTCGGCAAGCTGATTGTTGTCGCGt                                                                                     <  6:441968/135‑1 (MQ=255)
                                              gCATTGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTTGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTATCGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGt                                                                                     <  1:65366/135‑1 (MQ=255)
                                               cattGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCg                                                                                   <  7:196765/133‑1 (MQ=255)
                                               cAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCg                                                                                   >  7:111294/1‑136 (MQ=255)
                                                aaTGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTTGGCGGTGTTCTCGCGGCTCGAGGCGTAAGCTGCTCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCg                                                                                   <  5:312476/135‑1 (MQ=255)
                                                aaTGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCg                                                                                   <  5:319106/135‑1 (MQ=255)
                                                aaTGCGCTGCTTTTCGTCACGCATCGGGTGGGCTCTCATGGCGTAGGCGGGGTTCTGGATGCTCGTGGCTTCAGCTGCGCCGAGGTGGTATCGGCGCTGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCg                                                                                   <  6:43262/135‑1 (MQ=255)
                                                     gctgctTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGCTTGATGTCGCGTCGGATGGt                                                                             >  7:5239/1‑136 (MQ=255)
                                                               gTCACGCATCTGGGCGGCACGCATGGCGTAGGCGGGGGTCTCGATGCTCGAGGCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAg                                                                     <  2:354177/134‑1 (MQ=255)
                                                                    gCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTTGTAACGGCGCAGCAGGTTTAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGATTGACaa                                                               >  5:152340/1‑135 (MQ=255)
                                                                            tCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACa                                                         <  3:91875/133‑1 (MQ=255)
                                                                                 aCGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTgcgc                                                  <  3:219539/135‑1 (MQ=255)
                                                                                   gCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCa                                                >  1:312162/1‑135 (MQ=255)
                                                                                   gCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGGTGCCCTCGGCAAGCTGATTGATTTGCAGTCGGAAGGTCTGGGGAGTGACAACGAACAGCAGGGCCa                                                >  3:5610/1‑135 (MQ=255)
                                                                                    cATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCAt                                               <  3:348654/135‑1 (MQ=255)
                                                                                     aTGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTCAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCAttt                                             <  8:353046/136‑1 (MQ=255)
                                                                                     aTGGCGTAGGCGGTGTTCGCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCAttt                                             <  7:299013/136‑1 (MQ=255)
                                                                                            aGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCATGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGa                                       <  5:265928/135‑1 (MQ=255)
                                                                                                 gtgtTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTTGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTg                                  >  3:312930/1‑135 (MQ=255)
                                                                                                     tctcGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGAc                                >  6:441813/1‑133 (MQ=255)
                                                                                                     tctcGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAAAGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGAc                                >  6:75328/1‑133 (MQ=255)
                                                                                                     tctaGATGCTCGATTCGTAATCTGCGCCGCCCTTGTAACGGCGCAGCAGGTTGCTCTAGGCAACCAGATTTCAGTCGCGGAGGGATGTCTGGGGAGTGCCCACGAACACCTGCGCCATTTCCTAGATACTGaaa                               >  6:380341/1‑132 (MQ=255)
                                                                                                      ctcGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAg                            >  8:420325/1‑136 (MQ=255)
                                                                                                             cTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGt                      <  8:340321/135‑1 (MQ=255)
                                                                                                               ctaGTCGTAAGCTGCGCCGCCGTGTTAAAGTAGAATAAAGATAAGCTCGGCAAGCTGATTGAAGTCGCGTCGGATGGTCTGGGGAGTGACAAAGAACAGCTGCGCCATTTCCTCGATACTGACATAGCAGCGTTcg                   >  8:345010/3‑136 (MQ=255)
                                                                                                               cGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATTGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATAATGACATAGCCGCGTTcg                   >  7:391448/1‑136 (MQ=255)
                                                                                                               cGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACCACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTc                    >  6:66570/1‑135 (MQ=255)
                                                                                                                 aGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTcgc                  >  3:348500/1‑135 (MQ=255)
                                                                                                                   tCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGt                >  3:54883/1‑135 (MQ=255)
                                                                                                                   tCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTAGCGTCGGATGGTCTGGGGAGTGACAACGAACCGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGt                >  2:402455/1‑135 (MQ=255)
                                                                                                                         gCTGCGCCGCCGTTGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGt          <  4:312930/135‑1 (MQ=255)
                                                                                                                         gCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGt          <  4:72949/135‑1 (MQ=255)
                                                                                                                                cgccgTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGt          <  1:155367/128‑1 (MQ=255)
                                                                                                                                cgccgTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGt          >  2:155367/1‑128 (MQ=255)
                                                                                                                                cgccgTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGTTCGAGGa   >  4:224074/1‑135 (MQ=255)
                                                                                                                                cgccgTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGTTCGAGGa   >  3:204556/1‑135 (MQ=255)
                                                                                                                                cgccgTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGTTCGAGGa   >  4:295185/1‑135 (MQ=255)
                                                                                                                                 gccgTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGGCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGTTCGAGGAt  <  5:80892/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
GATGAACAGCGACGCATGGTCGGGAATCTGCCGGGCTACGGCGTCGGCAATGCGCTGCTTTTCGTCACGCATCTGGTCGGCACGCATGGCGTAGGCGGTGTTCTCGATGCTCGAGTCGTAAGCTGCGCCGCCGTGGTAACGGCGCAGCAGGTTGAGCTCGGCAAGCTGATTGATGTCGCGTCGGATGGTCTGGGGAGTGACAACGAACAGCTGCGCCATTTCCTCGATACTGACATAGCCGCGTTCGCGTACCAGTTCGAGGAT  >  KT2440/1226447‑1226710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑