Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,474,145 C→T 8.1% R216H (CGC→CAC)  rhlB_2 ← ATP‑dependent RNA helicase RhlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,474,1450CT 8.1% 143.3 / 2.6 49R216H (CGC→CAC) rhlB_2ATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base C (26/19);  new base T (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGAAGCCCATGTCGAGCATACGGTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCAGCTCGCGGGTGGGGGCGATG  >  KT2440/1474017‑1474279
                                                                                                                                |                                                                                                                                      
tGAAGCCCATGTCGAGCATACGGTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCtt                                                                                                                                  <  4:385016/135‑1 (MQ=255)
         tGTCGAGCATACGGTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCttt                                                                                                                        <  8:149553/136‑1 (MQ=255)
          gTCGAGCATACGGTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGAACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCttt                                                                                                                        <  2:403957/135‑1 (MQ=255)
             gAGCATACGGTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAgc                                                                                                                    <  8:147035/136‑1 (MQ=255)
                     ggTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTg                                                                                                              <  6:361660/134‑1 (MQ=255)
                             tCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCCCCAGCATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGACGTccc                                                                                                    >  8:444895/1‑135 (MQ=255)
                             tCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCa                                                                                                    >  7:134544/1‑136 (MQ=255)
                                  aaGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGGTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGcc                                                                                                >  3:330893/1‑135 (MQ=255)
                                        cATCACCTTGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACcgac                                                                                          >  5:270332/1‑135 (MQ=255)
                                            aCCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGc                                                                                     >  8:24258/1‑136 (MQ=255)
                                              cTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCtc                                                                                   <  8:48394/136‑1 (MQ=255)
                                               tCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCtc                                                                                   <  6:363367/135‑1 (MQ=255)
                                                 gcccATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCtcat                                                                                 >  6:112605/3‑135 (MQ=255)
                                                    cATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCAc                                                                              <  6:408459/135‑1 (MQ=255)
                                                     aTGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCtcatca                                                                               >  1:366080/1‑133 (MQ=255)
                                                     aTGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACg                                                                             >  5:5662/1‑135 (MQ=255)
                                                      tgtgCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGGAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGt                                                                            <  5:74370/135‑1 (MQ=255)
                                                         ccAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGTGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAg                                                                        >  7:137842/1‑136 (MQ=255)
                                                         ccAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGTGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAg                                                                        >  7:143962/1‑136 (MQ=255)
                                                         ccAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTc                                                                          <  5:278592/134‑1 (MQ=255)
                                                         ccAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTc                                                                          <  1:429840/134‑1 (MQ=255)
                                                            ggTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGc                                                                      <  6:31357/135‑1 (MQ=255)
                                                                cACTTCACCACGCTGGTTGAATTCCAGCAGGCGGCACGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCAATGCCACCGACGAAGCTCATCACGTTCAGGCCGgt                                                                  >  1:113173/1‑135 (MQ=255)
                                                                cACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCCAGTTCAGGCCGgt                                                                  >  1:214160/1‑135 (MQ=255)
                                                                cACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGgt                                                                  >  3:377126/1‑135 (MQ=255)
                                                                cACTTCACCACGCTGGTTGAAGTACAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCgga                                                                  >  6:238061/1‑134 (MQ=255)
                                                                 aCTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTTAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTGCAGGCCGgtg                                                                 <  5:112605/135‑1 (MQ=255)
                                                                 aCTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTGAGGCCGgtg                                                                 <  4:237151/135‑1 (MQ=255)
                                                                   ttCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCCTCCCGTTGAGGCCGGAGTa                                                               >  2:133141/1‑135 (MQ=255)
                                                                   ttCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTa                                                               >  1:113387/1‑135 (MQ=255)
                                                                     caccacGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTa                                                               >  1:416393/1‑133 (MQ=255)
                                                                     caccacGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACtt                                                            >  8:35474/1‑136 (MQ=255)
                                                                     caccacGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACt                                                             >  2:22857/1‑135 (MQ=255)
                                                                     caccacGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGCAGCCCCCAGAATATCGCAGTGGCGCGCTGCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGCCGAAGCTCATCACGTTCAGGCCGGTGTAcg                                                             >  4:330680/1‑134 (MQ=255)
                                                                      accacGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGGTCAGGCCGGTGTACtt                                                            >  1:227648/1‑135 (MQ=255)
                                                                       ccacGCTGGGTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACtt                                                            <  2:416393/134‑1 (MQ=255)
                                                                                ttGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCa                                                  >  4:369061/1‑135 (MQ=255)
                                                                                        cagcagGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGc                                                                      <  5:201406/107‑1 (MQ=255)
                                                                                        cagcagGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGc                                                                      >  6:201406/1‑107 (MQ=255)
                                                                                           cagGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTg                                      <  7:444895/136‑1 (MQ=255)
                                                                                            agGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGCGCTGCTTGTCGAAGTCCAAGCCACCGACGAAGCTCCTCACGTTGCGGCCGGTGTACTTCGTATGGGAAGCGCCGTCAttt                                      >  3:26980/1‑134 (MQ=255)
                                                                                            agGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTgg                                     >  8:310329/1‑136 (MQ=255)
                                                                                            agGCGGCCCGGCGTAGCCACCAGAATATCGCAGGGGCGCGCGTCCAGGGCGTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCATGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTg                                      <  5:251896/135‑1 (MQ=255)
                                                                                                          aGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGcaccac                        >  3:384458/1‑135 (MQ=255)
                                                                                                            ccaccaGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGGCCATGCCACCGACGAAGCGCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCAg                     <  7:406839/136‑1 (MQ=255)
                                                                                                             caccaGAATATCGCAGTGGTGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCAGc                    <  8:137842/136‑1 (MQ=255)
                                                                                                             caccaGAATATCGCAGTGGTGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCAGc                    <  8:143962/136‑1 (MQ=255)
                                                                                                                   aaTATCGCAGTGGCGCGCTT‑CAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCAGCTCGCgg              >  3:312798/1‑135 (MQ=255)
                                                                                                                    atatCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAATCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCCCCAGCTCGCgg              >  6:16688/1‑135 (MQ=255)
                                                                                                                                cgcgcTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCCGCTCGCGGGTGGGGGCgatg  >  1:111970/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                      
TGAAGCCCATGTCGAGCATACGGTCGGCTTCGTCAAGCACCATCACCTCGACCATGTCCAGGTGCACTTCACCACGCTGGTTGAAGTCCAGCAGGCGGCCCGGCGTAGCCACCAGAATATCGCAGTGGCGCGCTTCCAGGGCTTTGAGCTGCTTGTCGAAGTCCATGCCACCGACGAAGCTCATCACGTTCAGGCCGGTGTACTTGGTCAGGGCAGCGGCGTCCTTGGCGATCTGCACCACCAGCTCGCGGGTGGGGGCGATG  >  KT2440/1474017‑1474279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑