| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,508,654 | T→G | 33.2% | L178R (CTG→CGG) | ftsI → | Peptidoglycan D,D‑transpeptidase FtsI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,508,654 | 0 | T | G | 33.2% | 20.7 / 5.3 | 37 | L178R (CTG→CGG) | ftsI | Peptidoglycan D,D‑transpeptidase FtsI |
| Reads supporting (aligned to +/- strand): ref base T (10/13); new base G (9/4); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTGCTCGACCTCAAAGTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACC > KT2440/1508523‑1508788 | cGTGCGCGACCTCAAAGTGCCTGGTGGGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGGGACTGCGCACAGGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGc < 2:306790/135‑1 (MQ=255) tCGACCTCAAAGTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACCTGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGAGGGGTGGAGCGGGCCTAt > 5:279913/1‑134 (MQ=255) tCGACCTCAAAGTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGCCTATg > 8:445937/1‑135 (MQ=255) gACCTCAAAGTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGCGGGGGGGGGGTGGCCttt > 5:99055/1‑130 (MQ=255) tCAAAGTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGAGGGGTGGAGCGGGGCGATGAAGAGt > 3:95407/1‑135 (MQ=255) gTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGACGGGTGGGGGTGGGCTATTAATAGttggtt > 3:281994/1‑130 (MQ=255) tGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGAGGGGGGGGGGGGGGCGATGAAGAGTGGCTGGGCgg > 1:117361/1‑135 (MQ=255) ggTGTGTACGGACTGGAAGACTTCCGCCGCTGCTATCCGGCCGGTGATGTGCCTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGATGGGGGGGGGGGGGCGATGAAGAGTGGCTGGCCGGcc > 7:184844/1‑135 (MQ=255) ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGTGGGGTGGGGCGGGGCGATGTAGCGTGGCTGGCCGGGGGGCCCCGccaccg > 5:349210/1‑130 (MQ=255) ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGCAGGGGGGGAGCGGGGCGATGAAGACTGGCTGGCCGGCGGGCCCCGCCAgcg > 4:369167/1‑135 (MQ=255) ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGCGCTGGCCAATGCACACTGGCTGGCCCGCGGGCCCCGccaccc > 4:345281/1‑130 (MQ=255) ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGCGGTCGAGCAGGCCTATGAAGACTGGCTGGCCGGCCTGCCCGGCAAacg > 6:104677/1‑135 (MQ=255) tattaaTTCCGCGTCTCATAGCGGGCGGGTGATGTGACTCCGACCATGGGGGGCTTTA‑CCGACGGCCACGACCACGGTCGTGAAGGGGTGGAGCTGGCCTATCAAGAGTGGCTGGCCGGCGTGCCCGGAAAGCGcc < 8:96327/132‑1 (MQ=255) aataaTGCCGCCGCTTCAATCCGGCCGGTTCGGTCACGGCGAACAGGGGGGGGTTAC‑CCCACCGCGACGACCACGGGCGTGAAGGGGTGGAGCTGGCCTATGAAGAGTGGCGGGCCGGCGTGCCCGGCAAGCGcc < 6:279913/132‑1 (MQ=255) aagaaTTCCGCCGTTTCTAACCGGCCGGTGATGGGCCTGGGCACAGGGGCGGCTTTC‑CCGACCGCGACGACCACGGTCGTGATGGGGTGGAGCTGGCCTATGACGAGGGGCTGGCCGGCGTGCCCGGCAAGCGcc < 6:122510/135‑1 (MQ=255) aaTTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGGAGGGGTGGCGCTGGGCGATGTAGAGTGGCTGGCCGGCGTGGCCGGCCAGCGCCCgg > 1:92985/1‑135 (MQ=255) tttCCGCCGCTTCTTCCGGGCCGGGGAGGTGACTGCGCACTTGGTGGGCTTTC‑CCGACCGCGACGACCACGGTGGTGATGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTg < 7:445937/135‑1 (MQ=255) cgccgcTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGCAGGGGTGGGGCGGGCCTATGAAGAGTGGCTGGCCCGCGGGGCCGGCACGCGCCAGGTGAt > 2:342950/1‑134 (MQ=255) gccggTGATGTGCCTGCGCACTTGGGCGGCTTAC‑CCGACGTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGCCAATGAAGAGTGGCTGGCCGGGGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGt < 1:83290/135‑1 (MQ=255) gttcgtGCGCACATGGT‑GGTTTTACCCTACGTGGACGCCCTCGGGCGGGGCGGGGGGGAGCTGGCCTATGAAGAGGGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGAt < 1:57690/129‑1 (MQ=255) gcgcACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTg > 8:323015/1‑102 (MQ=255) gcgcACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTg < 7:323015/102‑1 (MQ=255) ggCTTTC‑CCGACGTCGAGGACCACGGTGGTTATGGGTGGGTGGTGGCGTTTGATGTGTGGGTGGCCGGGGTGCCGGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAAcc < 2:241634/135‑1 (MQ=255) cTTTA‑CCGACCTCGACGACCACGGTCGTGCAGGGGTGGGGCGGGCCTATGAAGAGTGGCTGGCCCGCGTGCCCGGCCAGCGCCAGGTGAGCAAGGCCCGGCGCGGGCGGCTGATGAAGGGCATTGAGGTAAc > 4:319154/1‑132 (MQ=255) ccGACCTCCACGCCCCCGGTCGTGAAGGGGTGCCGCTGGCCTATGAAGCGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACg < 3:117569/135‑1 (MQ=255) cgacggcgacgaCCACGGTCGTTAAGGGGGGGAGCGGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACg < 4:95407/128‑1 (MQ=255) tagccccAGGGTCGTTAAGGGGGGGAGCTGGCCTATGACTTGGGGCGGGCGGGGGGGCCCGGAAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCACGGCCg < 7:294709/131‑1 (MQ=255) gacgacCACGGTCGTGAAGGGGGGGAGCGGGCCGATGAACTGTGTCTGGGCCGCGGGGCCGGCCCGCGCCACGTGATGAACGCCCCGCGCGGGGGGGGGAGCACGGCTCTTGAGGGAACCAAGAACGCCacagcc > 6:32000/1‑130 (MQ=255) acgacCACGGTCGTGACGGGGTGGAGCGGGCCTATGAAGAGGGGCTGGCCGGCGGGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCg < 3:241816/135‑1 (MQ=255) tGATGGGGGGGCGCGGGCTTATTACGAGTGGCGGCCGGGCGTGCCCGGCAAGCGCCAGGTGAGCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGGACCCAAGAACGCCAAGGCCGGGAAGACCTTGGc < 3:72434/135‑1 (MQ=255) tGACGGGGGGGAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc < 6:220986/135‑1 (MQ=255) ggacggggTGGAGCGGGCCTATGAAGAGTGGCTGCCCGGCGGGCCCGTCAAGCGCCAGGTGATCACGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc < 4:31449/131‑1 (MQ=255) tatggggTGGCGCTGGCCTATGACGAGTGGCTGGCCGGGGGGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc < 7:78741/131‑1 (MQ=255) gAGCTGGCCTATGAAGATTGGCTTGCAGACGTGCCCGGAAAGCGACAGGTGATCAAGGACCGGCGCGGTCGGCTGATAAAGGACATTCAGGTAACCAAGAAAGCCAATGCAGGGAAGACCTTGTCGTTGTCGAt > 6:420067/1‑134 (MQ=255) gAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCg > 4:361118/1‑132 (MQ=255) gAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGa > 7:3760/1‑133 (MQ=255) cTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGAcc > 8:353251/1‑136 (MQ=255) tGGCCTATGAAGAGGGGCGGGCCGGCGGGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGAcc < 4:98428/135‑1 (MQ=255) | CGTGCTCGACCTCAAAGTGCCTGGTGTGTACGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTA‑CCGACCTCGACGACCACGGTCGTGAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCGGCGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACC > KT2440/1508523‑1508788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |