Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,528,308 A→C 29.1% P87P (CCA→CCC)  argJ → Arginine biosynthesis bifunctional protein ArgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,528,3080AC29.1% 59.2 / 6.7 53P87P (CCA→CCC) argJArginine biosynthesis bifunctional protein ArgJ
Reads supporting (aligned to +/- strand):  ref base A (27/9);  new base C (15/2);  total (42/11)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGC  >  KT2440/1528177‑1528436
                                                                                                                                   |                                                                                                                                
aGCGTGGCTGGCGTGTTCACCCTCCACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCCACACGGGTAACGCCAACGCCGGCACCCGCGCGCCCGGc                                                                                                                               >  5:358690/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCCCCGGCGCGCCAGGcc                                                                                                                              >  7:364579/1‑136 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGc                                                                                                                               >  5:3875/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGcc                                                                                                                              >  8:42142/1‑136 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGcc                                                                                                                              >  8:352668/1‑136 (MQ=255)
  cGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGccg                                                                                                                             >  2:339385/1‑134 (MQ=255)
  cGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCt                                                                                                                             >  6:348113/1‑135 (MQ=255)
       cgggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGGCCAAACAGCGTGTACAGGGCACCGGGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGgccg                                                                                                                        <  6:194159/133‑1 (MQ=255)
        tggcGGGTTCACCCTCACCGCCTTCTGCGCGGCTCCGGTGATACTGTCCACACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACCGGTAACGCCAACGCCGGCCCCGGCGCGCCCCGCCTGgcccac                                                                                                                      >  7:6766/1‑133 (MQ=255)
         ggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGgccgccg                                                                                                                     >  8:20053/1‑136 (MQ=255)
                  aCCCTCAACGCCTGCTGCGCGGCGCGGGTGATCCTGTCAAAACAGCGTGTACAGGGCAGCGTGCTCTACCTGCTGACCAACCCGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAgcgc                                                                                                             <  2:268628/135‑1 (MQ=255)
                                 tGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTg                                                                                              >  6:335839/1‑135 (MQ=255)
                                 tGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCCGCCTGGCCGCCGCCGCGCGCCCCAGCGCCAAGcag                                                                                              >  6:67349/1‑133 (MQ=255)
                                  gcgcGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTgg                                                                                             >  3:317265/1‑135 (MQ=255)
                                                     gTCCAAACCGCGTGTACAGGGCAACGTGCGCTACCTGCTGACCACCACGGGTAACGCCAACGCCGGCACCCGCGCGCCAGGCCTGGCCCCCGCCGAGCGCCCCTGCGCCAAGCTGCCCGAACTGGCTTGCGTAcc                                                                          >  3:176225/1‑135 (MQ=255)
                                                     gTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCCCGCCCCTGCGCCAAGCTGGCCGAACAGGCTGGCGTAcc                                                                          >  1:178418/1‑135 (MQ=255)
                                                     gTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCGGGCCGCCGCCCAGCGCCCCTGCGCCCACCTGGCCGCCCTGGCGGGCGTAcc                                                                          >  5:247183/1‑135 (MQ=255)
                                                     gTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                          >  6:207058/1‑135 (MQ=255)
                                                        cAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGc                                                                       >  3:359548/1‑135 (MQ=255)
                                                            cAGCGTGAACCGGGCACCGGGCGCTACCTGCTGACCAACCCGGGAAACGCCACCGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGt                                                                  <  8:364579/136‑1 (MQ=255)
                                                              gCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCg                                                                >  7:334923/1‑136 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGATCTGGCTGGCGTACCGGCCGAGTCGGTa                                                             >  3:27072/1‑135 (MQ=255)
                                                                   tACAGGGCACCGTGCGCTACCTGCTGCCCAACACCGGTAACGCCAACGCCGGCACCGGCGCGCCCCCCCTGGCCCCCGCCGAGCGCACCGGCGCCAACCTGGCCGAACTGGCTGGCCTACCGGCCGACTCGGTAc                                                            >  5:227433/1‑135 (MQ=255)
                                                                   tACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACTCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGCACCGGCCGAGTCGGTAc                                                            >  5:193728/1‑135 (MQ=255)
                                                                   tACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACt                                                           >  8:113716/1‑136 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACCCGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCGGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACGGCCggt                                                     >  8:70663/1‑133 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCCAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTAGGTACAGCCGtt                                                     >  8:221627/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCAGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGt                                                      >  4:351997/1‑134 (MQ=255)
                                                                           aCCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAGCGGCCGAGTCGGTACTGCCGTtc                                                    >  3:373063/1‑135 (MQ=255)
                                                                           aCCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGGCGGTACTGCCGTtc                                                    >  4:66188/1‑135 (MQ=255)
                                                                            ccGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAACGGCCGAGTCGGTACTGCCGTtct                                                   >  4:200860/1‑135 (MQ=255)
                                                                              gTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTcc                                                 >  4:188080/1‑135 (MQ=255)
                                                                               tGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCGGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGGCGGTACTGCCGTTCTCCa                                                >  3:81656/1‑135 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCGGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGGCGAGTCGGTACTGCCGGTCTcccc                                               >  2:334198/1‑133 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCAcc                                              >  7:377459/1‑136 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGGACTGGCTGGCGTTCCGGCCGCGTCGGTACTGCCGTTCTCCAccc                                             >  6:392025/1‑134 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCg                                             >  4:443756/1‑135 (MQ=255)
                                                                                  gcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCCCCAACCAGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGGACTGCCGGTCTCCAcccg                                            >  7:428168/1‑134 (MQ=255)
                                                                                         gctgACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCCCCCCAGCGCCAACCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGGTCTCCCCCGGCGTggg                                      >  3:25821/1‑133 (MQ=255)
                                                                                           gcaccaccACGGGACCCGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGAt                                      <  1:338908/131‑1 (MQ=255)
                                                                                              ccAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGCACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGc                                  <  6:193728/134‑1 (MQ=255)
                                                                                                 acacGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAACCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGgcgcgc                              >  4:194773/1‑135 (MQ=255)
                                                                                                    cGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGCGTCGGTACTGCCCTTCTCCCCCGGCGGGCTTGGCGGGcccc                           >  4:57178/1‑133 (MQ=255)
                                                                                                          acgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGGTTTGGGCGCCgcgggcgg                     >  3:259773/1‑135 (MQ=255)
                                                                                                           cgcccacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTc                   <  7:147526/136‑1 (MQ=255)
                                                                                                             ccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTATCCACCGGCGGGATTGGCGTGCCCCTGCCgggc                   >  4:316715/1‑132 (MQ=255)
                                                                                                                 ccccGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGaaaa              <  5:342994/133‑1 (MQ=255)
                                                                                                                   ccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCAACGGCGTGATTTGCGAGCCGCTGCCGGGCGaa                >  7:433240/1‑131 (MQ=255)
                                                                                                                    cgcccccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGAt           <  5:417801/130‑1 (MQ=255)
                                                                                                                        aCCGGCGCGCCAGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGaa       <  4:56799/135‑1 (MQ=255)
                                                                                                                         ccgccgCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg      <  5:348113/135‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGCTTGGGGCGCCCCGGGCCGGCGCAAAGATCGAAg      >  6:423716/1‑135 (MQ=255)
                                                                                                                             cgcgcCCGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGgcgc  <  5:370273/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                
AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGC  >  KT2440/1528177‑1528436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑