Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,544,643	T→G	22.3%	Q412P (CAG→CCG)	pykA ←	Pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,544,643	0	T	G	22.3%	80.4 / 3.1	74	Q412P (CAG→CCG)	pykA	Pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base T (13/41);  new base G (13/5);  total (28/46)
Fisher's exact test for biased strand distribution p-value = 4.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTGGCAG  >  KT2440/1544512‑1544769                                                                                                                                    |                                                                                                                               cccAGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGt                                                                                                                            >  8:14605/1‑136 (MQ=255)   cAGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTgg                                                                                                                          <  8:32144/136‑1 (MQ=255)   cAGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGGTCGTCGACCGCCGCCTGGCTCCCCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTgg                                                                                                                          <  7:310478/136‑1 (MQ=255)    aGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGgc                                                                                                                         >  8:268580/1‑136 (MQ=255)    aGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGgggggg                                                                                                                          >  5:210723/1‑135 (MQ=255)    aGTCACCTTGCTCCACCAGGCCACGTTTCAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTgg                                                                                                                          <  1:310892/135‑1 (MQ=255)       cACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGcgcg                                                                                                                      <  8:380417/136‑1 (MQ=255)         ccTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGCAGGCAATCGGGTACACGCCCCGGAACATCGAGGCGCGCGCCGGGGGGTCGCggg                                                                                                                    >  7:173344/1‑135 (MQ=255)         ccTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGCGGCGCGCGGCAGGGTGGCGCgg                                                                                                                     >  1:358479/1‑135 (MQ=255)         ccTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCgg                                                                                                                     >  4:29815/1‑135 (MQ=255)         ccTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCgg                                                                                                                     >  2:7052/1‑135 (MQ=255)              cTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTgcgg                                                                                                                <  8:99855/135‑1 (MQ=255)               tCCCCCCGGCCCCGTTTGCGCAGGTCGGCGACCGCCGCCTGGCTCCCCTTATCGGCCGGCAGCGCGGGCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGCGGCGCGCGCCTGGGTGGCGCGGTgcggc                                                                                                               <  3:295586/135‑1 (MQ=255)               tCCACCAGGCCACGTTTGAGCAGGTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGTAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTgcgg                                                                                                                <  3:71609/134‑1 (MQ=255)                   ccaGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCCGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGACGCGCGCGCCCGGGTGGCGCGGGggggcgcga                                                                                                           >  3:321817/1‑132 (MQ=255)                   ccaGGCCACGTTTGAGCAGGTCGGCGCCCGCCGCCGGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGACa                                                                                                           <  1:316310/135‑1 (MQ=255)                     aGGCCACGTTTGCGCCGTTCGTCCCCCGCCGGCTGGCGCACCTTATCGGCCGGCCGCGCGGCCGGGGCGAAGGCAATCGGGAACACGCCACGGAACACCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAgg                                                                                                         <  5:440367/135‑1 (MQ=255)                      ggCCACGTTTGAGCAGTTCGTCGCCCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTCCACGCCACGGAACATCGCGGCGCGCGCCTGGGGGGCGCGGGGCGGCGCCCGGGc                                                                                                       >  8:134245/1‑136 (MQ=255)                      ggCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCCCCGCGGTGGCGCGGTGCGGCCCCAGGGc                                                                                                       >  8:423412/1‑136 (MQ=255)                       gCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGACAGGGc                                                                                                       >  6:169218/1‑135 (MQ=255)                       gCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGCGGTGGCGCGGTGCGGCGCCAGGGc                                                                                                       >  2:120227/1‑135 (MQ=255)                         cACGTTTGAGCAGTTCGTCGACCCCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGCCGAACGCAATCGGGTTCACAGCACGGAACATCGACGCCACAGCCGGGCTGGCCCGGTGCGGCGGCCAGGCGa                                                                                                     >  6:82608/1‑135 (MQ=255)                         cACGTTTGAGCAGTTCGGCGACCGCCGCCTGGCTCAGCTTCTCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTCCACGCCACGGAACATCGAGGCTCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGaa                                                                                                    <  7:257923/136‑1 (MQ=255)                          aCGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGaa                                                                                                    <  1:242735/135‑1 (MQ=255)                          aCGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGCAg                                                                                                   >  8:352383/1‑136 (MQ=255)                          aCGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGGCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGaa                                                                                                    <  2:337691/135‑1 (MQ=255)                          aCGTTTGAGCAGTTCGTCGACCGCCGCCTGGCGCACCTTCTCGGCCGGCAGCGGGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGaa                                                                                                    <  6:383458/135‑1 (MQ=255)                                         gtcgACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGCAGGCAATCGGGTACACGCCCCGGAACATCGCGGCGCGCGCCTGGGTGGTGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGa                                                                                     >  5:105802/1‑135 (MQ=255)                                         gtcgACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCCCGGAACATCGAGGCGCGCGGCGGGGTGGCGCGGGGCGGCGGCAGGGCGAAGATCGGTACATGCgg                                                                                     >  4:76039/1‑134 (MQ=255)                                         gtcgACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGCCAGGGCGCAGCTCGGTACATGCGa                                                                                     >  4:199611/1‑135 (MQ=255)                                           cgACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAAc                                                                                   <  4:85473/135‑1 (MQ=255)                                              ccgccgccgGGCTCACGTTCTGGGCCGGCCGGGCGGGCGGGTCGATGGCAATCGGGAACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCa                                                                                <  1:398437/135‑1 (MQ=255)                                              ccgccgCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCa                                                                                <  6:210723/135‑1 (MQ=255)                                               cgccgcCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAg                                                                               <  1:2728/135‑1 (MQ=255)                                                  cgccTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGGACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGgcgc                                                                           <  7:347394/136‑1 (MQ=255)                                                   gccTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGTAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGgcgc                                                                           <  3:406195/135‑1 (MQ=255)                                                           ccgcTTAGCGGCCGGCCGCGCGGCCGGGTGTAAGGCAATCGGGTCCCCGCCACGTAACATGGAGGCGCGCGCCTGGGGGGCGCGGTGCGGCGACAGGGCGAAGAGCGGTACATGCGAACGCAGTCGCGACATGAt                                                                   <  4:436916/132‑1 (MQ=255)                                                            aCCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACTTGATCa                                                                 <  7:423412/136‑1 (MQ=255)                                                             cgttAGCGGCGGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCa                                                                 <  2:201819/133‑1 (MQ=255)                                                             ccTTCTCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGGGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCa                                                                 <  2:334841/135‑1 (MQ=255)                                                             ccTTATCGGCCGGCTGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGCGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCa                                                                 <  4:20000/135‑1 (MQ=255)                                                             ccTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCa                                                                 <  4:368462/135‑1 (MQ=255)                                                             ccTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCa                                                                 <  4:230184/135‑1 (MQ=255)                                                               ttATCGGCCGGCAGCGCGGCGGGGTCGAAGGCAATCCGGTACACGCCACGGACCATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCCCCCGGGCGAAGATCGGTACCTGCGAACGCCGGCCCGACCTGATCAGc                                                               >  6:103004/1‑135 (MQ=255)                                                                 aTCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGGGGCGCCGTGCCGCGGCAGGGCGCAGATAGGTTCAAGCGAACCCCGGCGCCCCATGGTAAGccg                                                             >  5:160146/1‑133 (MQ=255)                                                                 aTCGGCCGGCAGCGCGGCCGGGGCGAAGGCAATCGGGTACACGCCACGTAACCTCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCgg                                                             <  8:419601/135‑1 (MQ=255)                                                                     gccggcAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGCCAGGGCGAAGATCGGTACATGCGCACGCAGGCGCGACATGATCAGCGGGgtgt                                                        >  7:331879/1‑136 (MQ=255)                                                                           aGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGCTCGGTACATGCGCACGCAGGCGCGACATGCTCAGCGGGGTGTATCCg                                                   >  4:142685/1‑135 (MQ=255)                                                                            gcggggcgggGGCGACGGCAATCGGCTACACCCCCCGCAACTTGGCGGCGCGCGCCTGGGGGGCGCGGGGGGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGc                                                  <  3:423168/135‑1 (MQ=255)                                                                                       tCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAgg                                       <  3:348250/135‑1 (MQ=255)                                                                                        cGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAggg                                      <  3:136250/135‑1 (MQ=255)                                                                                            ggCTATCGGGTACACGCCACGGCACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAATATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCgat                                  <  5:59168/135‑1 (MQ=255)                                                                                               aaTCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCGGTGGGGCGACAGGGCGAAGCTCGGTACATGCGCACGCCGGCGCGCCATGGTCAGCGGGGTGTAGCCGCGTTGCGTCAGGGCgatgat                               >  5:302183/1‑135 (MQ=255)                                                                                               aaTCGGGTACACGCCACGGAACATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATc                              >  8:360381/1‑136 (MQ=255)                                                                                                   gggTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGCCAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCt                          >  8:239440/1‑136 (MQ=255)                                                                                                   gggTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACCTGATCAGCGGGGTGGTGCCGCTTTCGGTCATGGCGATGATCGcc                           >  4:136250/1‑135 (MQ=255)                                                                                                    ggTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCt                          <  3:76039/135‑1 (MQ=255)                                                                                                              aCGGAACATCGCGGCGCGCGCCTGGGGGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCgg                <  3:29815/135‑1 (MQ=255)                                                                                                                  aaCATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGt           <  7:285748/136‑1 (MQ=255)                                                                                                                  aaCATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGCCCGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATAAGCGGGGTGTAGCCGCTTTAGGTGAGGGCGATGAAAGCCGTTTCGCCCGGGAAGt           >  8:241518/1‑136 (MQ=255)                                                                                                                   ccATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGt           <  1:120227/134‑1 (MQ=255)                                                                                                                   agaTCGTGGGGCGGGCCTGGGTGGGGCGGTGCTGCGACTGGGCGTAGAGCGGTAGATGCGATCGCAGGCGCGACATGATGAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGt           <  5:82608/133‑1 (MQ=255)                                                                                                                   aCATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGt           <  1:242087/135‑1 (MQ=255)                                                                                                                   aCATCGAGGCGCGCGCCGGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGt           <  5:169218/135‑1 (MQ=255)                                                                                                                      tCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGt        <  3:142685/135‑1 (MQ=255)                                                                                                                      gcgcggCGCGCGCCGGGGGGGCGCGGTGCGGCGACAGGGCGAAGAGCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGt        <  2:195298/131‑1 (MQ=255)                                                                                                                        gtggggcgcgcCGGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGt        <  2:420856/128‑1 (MQ=255)                                                                                                                        gcggCGCGCGCCTGGGTGGCGCGGGGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTg      <  6:20896/133‑1 (MQ=255)                                                                                                                        gAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTg      <  7:268580/135‑1 (MQ=255)                                                                                                                        gAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTg      <  8:30429/135‑1 (MQ=255)                                                                                                                         aGGCGCGCGCGTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTg      <  1:62942/134‑1 (MQ=255)                                                                                                                         aGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTg      <  3:199611/134‑1 (MQ=255)                                                                                                                          ggCGCGCGCCTGGGTGGCGCGGTGCGGCGCCAGGGCGCAGCTAGGGTCATGCGCACGCAGGCGCGACATGTTCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGAATATCGCCTTTACGCCCGGGAAGGGGTTg      >  4:188770/1‑133 (MQ=255)                                                                                                                            cgcgcgcCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATAGCCTTTACGCCCGGGAAGTGGTTGGCAg  >  6:194552/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                               CCCAGTCACCTTGCTCCACCAGGCCACGTTTGAGCAGTTCGTCGACCGCCGCCTGGCTCACCTTATCGGCCGGCAGCGCGGCCGGGTCGAAGGCAATCGGGTACACGCCACGGAACATCGAGGCGCGCGCCTGGGTGGCGCGGTGCGGCGACAGGGCGAAGATCGGTACATGCGAACGCAGGCGCGACATGATCAGCGGGGTGTAGCCGCTTTCGGTCAGGGCGATGATCGCCTTTACGCCCGGGAAGTGGTTGGCAG  >  KT2440/1544512‑1544769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑