| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,892,029 | G→C | 24.5% | intergenic (+43/‑477) | PP_RS08755 → / → napA | tRNA‑Asp/Periplasmic nitrate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,892,029 | 0 | G | C | 24.5% | 24.1 / 3.6 | 39 | intergenic (+43/‑477) | PP_RS08755/napA | tRNA‑Asp/Periplasmic nitrate reductase |
| Reads supporting (aligned to +/- strand): ref base G (17/10); new base C (2/7); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTC > KT2440/1891896‑1892162 | acTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACAGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCAGCGCCATATTACACATACACGGGCCACAGAACACCCGGAAGTAAAaaag > 5:177411/1‑134 (MQ=11) aTGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGGACACCCCGAAGTAAAAAAGAAAGCGa > 8:293258/1‑136 (MQ=17) tataGCGCAGCGGTAGTTCAGTCGG‑TAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCGGCGCCCTATTACACTTGCAGGGCCCCCTGACCCCCCCGAACTAAAAAAGAAAGCCAccg > 7:234449/1‑134 (MQ=11) gcgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCCGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGCCCTTAg > 4:188238/1‑135 (MQ=17) gcgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCGGCGCCCTATTCCACTTCCAGGGCCCCCTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAgg > 7:84300/1‑136 (MQ=16) gcgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTACCCTGCGCCCTATTCCACTTCCCGGGCCCACTGTCCACCCGGCAGTAAAAAAGAAAGCGCCCtt > 4:323521/1‑133 (MQ=11) gcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGGCATATTCCACTTCCCGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAggg > 3:227098/1‑135 (MQ=18) gcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGGGCCCGGCGCCATATTACACCTCCAGGGCCCCCGGACACCCCCGAAGTAAAAAAGAAAGCGCCCGTAggg > 1:265236/1‑135 (MQ=11) cAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCCCTGAACCCCCGGAAGTAAAAAAGAAAGCGACCTTAGGGt > 4:355007/1‑135 (MQ=21) cAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCGGCGCCATATTCCACTTCCAGGGCCCCCTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGt > 5:148800/1‑135 (MQ=21) cAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCAGGGGCATATTGCACGTCCAGGGCCCACTGGACACCCGGAAGTAAAAAAAAAAGCGCCCATAgggg > 1:15691/1‑134 (MQ=11) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTGGAGTCCCGTGCCGAGCGCCAAATTACCCTTCCCGGGCCCCCGGAACACCCCGTCGTAAAAAACAAAGCCCCCGTAgggggggg > 6:75516/1‑130 (MQ=2) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGCGTCCCGTCCGCTGCGGCGTATTCCACTTCCAGTGGCCCCTGAACCCCCGGCACTAAAAAACAAAGCCACCATAGGGTCGcg > 6:175289/1‑134 (MQ=11) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTGCGCGGCGCCCAATTCCACGTCCCGGGGCCCCGGCACACCCCGGAGTAAAAAAGAAATCCACCGTATGGtgggg > 5:398572/1‑131 (MQ=2) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGGTGCGCCATATTCCACGTCCAGGGCCCCCTGCACACCCGGAAGTTAAAAAGAAAGCGCCCTGAGGGTCGcg > 1:89985/1‑134 (MQ=11) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCCCTGCACACCCGGAAGTAAAAAAGAAAGCGCCCTTAGGGTCGcg > 6:336808/1‑134 (MQ=255) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCCCTGAACCCCCGGAAGTAAAAAAGAAAGCGTCCGTAGGGGCGCtg > 8:28749/1‑135 (MQ=11) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTACACTTCCAGGGCCCCCTGAACCCCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTAGcg > 5:195065/1‑134 (MQ=255) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATAATCCACTTCCAGGGCCCCCGGAACACCCGGAAGTAAAAAAGAAAGCGACCGGAGGGTCGcgg > 7:443321/1‑134 (MQ=11) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCGGCGCCATATTCCACTTCCAGGGCCCACTGCACACCCCGAAGTAAAAAAGAAATCGACCTTAGGGTCGcg > 3:213679/1‑134 (MQ=255) ggTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGGTCGAGTCCCGTCCGCTGCGGCATATTACACTTCCAGGGCCCACTGCGCACCCGGGAGTAAAAAAGAAATCGCCCTTAGGGTCGcg > 6:155968/1‑134 (MQ=11) tAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGGTCGAGTCCCGTGCCCTCCGCCCGATTACACCTACAGGGCCCACTGCACACCCGGAAGTAAAAAATAAATACACCTGTGGGTCGcggg > 2:368502/1‑132 (MQ=11) aGTGGGTTCGAATACCGGCCGGCCACGCCGGGGGGCGCGGGGGCGAGTCCCGGCCGCGGCGCCAAATTCCACTTCCCGGCCCCACTGAACACCCGGAAGTAAAAACGAAAGCGACCTTAGGGTCGCTTTTTTTa < 2:89985/134‑1 (MQ=11) gggTTACAATCCGGGCCGGGCACGCCGGGGGGGGGGGGTTCGAGTCCCGGCCGCTGCGCAATATTCCACTTCCCGGGCCCACTCAACACCCGCAATTAAAAAAGAACGCGACCTTAGGGTCGCTTTTTTTATTTCt < 8:84300/135‑1 (MQ=255) tcccGGCGTGTCACGCGGGGGGTCGCGGGTGCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTTAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGcatcat < 6:160279/132‑1 (MQ=14) tCAGGCGGGGGGTCGCGGGTTCGAGGCCCGGCCGCTGCGCCATATTCCACTTCCCGGGCCCACTGAACACCCGGAAGTAAAACAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTAc < 7:293258/136‑1 (MQ=18) tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCAATATTCCACTTCCAGGGCCCACTGAACACCCGCAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTAc < 7:70754/136‑1 (MQ=255) cccgcccgCCGCCCCAAATCCCCCACCCCGGCCCCCCTCAACACCCGCACTAAAAAAACACCGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTgcg < 1:183434/135‑1 (MQ=255) gcCATATCCCCCATCCCGGGCCCACTGAACACCCGTAAGTAAAAAAGACCGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTcc < 8:244548/136‑1 (MQ=37) gcCATAATCCACTGCCCGGCCCCACTGACCACCCGGAAGTAAAAAACAACGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTcc < 7:106287/136‑1 (MQ=255) cccTTCCAGGCCCCACAGACCACCCGCAACTAAAAACCACCGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGt < 5:347271/133‑1 (MQ=255) cACTTCCCGGGCCCACTCACCACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGt < 4:395138/135‑1 (MQ=255) cACTTCCAGGGCCCACTGAACACCCGCAAGTAAAAAACAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGt < 6:148800/135‑1 (MQ=255) cACTGCCCGGACCCACTCAACACCCGGAAGAAAAAACCAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGt < 4:442553/135‑1 (MQ=255) cccGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGa < 8:443321/136‑1 (MQ=255) ccGTAAGTAAAAAAGAAAGCGACCTTATGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCGCGCAGCGTCAGCACCTCAAACCCGTCCTCGa < 3:155072/135‑1 (MQ=255) attAAAAAACAACGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCAcc < 5:336808/133‑1 (MQ=255) ttAAAAAATAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCg < 2:265236/134‑1 (MQ=255) caaaaataGACAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCg < 3:326188/133‑1 (MQ=255) aaaaCAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCAtc < 7:408334/136‑1 (MQ=255) aaataaagCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCAtc < 3:355007/135‑1 (MQ=255) aaagaaagCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCAtc < 1:349716/135‑1 (MQ=255) cgaaagCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCAtctc < 6:291161/134‑1 (MQ=255) | ACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTC > KT2440/1891896‑1892162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |