Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,949,630	G→A	6.3%	P54P (CCG→CCA)	CJ019_01863 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,949,630	0	G	A	6.3%	193.6 / 3.3	63	P54P (CCG→CCA)	CJ019_01863	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/34);  new base A (2/2);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.10e-01

GGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGTGACGAGGAATATG  >  KT2440/1949496‑1949754                                                                                                                                       |                                                                                                                             ggTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgc                                                                                                                             <  8:198069/136‑1 (MQ=255)  gTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgc                                                                                                                             <  4:184235/135‑1 (MQ=255)  gTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgc                                                                                                                             <  4:288320/135‑1 (MQ=255)  gTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgc                                                                                                                             <  4:60415/135‑1 (MQ=255)  gTATAAAGGTAAATGTGAATTACCTTGAGAGGTGCGTGCGTCATACTTTGCCTCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGGTCCGAAGCcgc                                                                                                                             <  4:280066/135‑1 (MQ=255)     taAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgcgc                                                                                                                           <  2:218430/134‑1 (MQ=255)         ggTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGATCCGAAGCCGCGCGCTGTa                                                                                                                     <  8:386004/136‑1 (MQ=255)           tAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAg                                                                                                                    >  6:227912/1‑135 (MQ=255)                gtgAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAATAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAt                                                                                                               >  1:389559/1‑135 (MQ=255)                gtgAATTACCTTGAGAGGTTCGTGCGGCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAt                                                                                                               >  6:65119/1‑135 (MQ=255)                 tgAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAtt                                                                                                              <  8:231008/135‑1 (MQ=255)                  gAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAtt                                                                                                              <  5:385969/134‑1 (MQ=255)                  gAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAtt                                                                                                              <  5:227912/134‑1 (MQ=255)                  gAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAtt                                                                                                              <  5:196637/134‑1 (MQ=255)                  gAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAtt                                                                                                              <  3:31991/134‑1 (MQ=255)                  gAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTg                                                                                                             <  6:299956/135‑1 (MQ=255)                      tACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAg                                                                                                        >  7:276273/1‑136 (MQ=255)                       aCCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGc                                                                                                       >  7:50591/1‑136 (MQ=255)                       aCCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGc                                                                                                       >  7:309666/1‑136 (MQ=255)                       aCCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGc                                                                                                       >  7:57808/1‑136 (MQ=255)                          ttGAGAGCTTCGTGCGGCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCtt                                                                                                     <  4:442681/135‑1 (MQ=255)                            gagaGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCtt                                                                                                     >  1:18674/1‑133 (MQ=255)                                ggTTCGTGCGTCATAATTTGCATCGTGAACCTTGTAAGAGTTCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATagcc                                                                                               <  3:274758/135‑1 (MQ=255)                                ggTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATagcc                                                                                               <  3:303231/135‑1 (MQ=255)                                ggTTCGTGCGTCATAATTTGCATCGTGAACATTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATag                                                                                                 >  3:427712/1‑133 (MQ=255)                                     gTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATagccagcc                                                                                           >  2:259418/1‑134 (MQ=255)                                      tGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCg                                                                                          <  1:151599/134‑1 (MQ=255)                                               aTTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGgt                                                                                >  6:66990/1‑135 (MQ=255)                                               aTTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGgt                                                                                >  5:339075/1‑135 (MQ=255)                                                tttGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGgt                                                                                <  3:46029/134‑1 (MQ=255)                                                            aCCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAAc                                                                   >  6:143235/1‑135 (MQ=255)                                                               ttGGAAGAGTGCAATTGAAACAGTCCTGAATGACGATTTGAAAAGAGCAGATCAAGACTGTGGTCCGAAGCAGCGCGCTGTAGTGGATGGCGGAGCGTATAGCCAGCCTGCTTGTGGGTGTACTGATCAAACCCg                                                                <  4:69024/135‑1 (MQ=255)                                                                  gAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGAt                                                            <  8:18594/136‑1 (MQ=255)                                                                  gAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGAt                                                            <  8:276273/136‑1 (MQ=255)                                                                   aaGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCACGCGCTGTAGTGGAtt                                                                                                              >  2:68955/1‑85 (MQ=255)                                                                   aaGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCACGCGCTGTAGTGGAtt                                                                                                              <  1:68955/85‑1 (MQ=255)                                                                      agTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGACCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGcc                                                        <  8:439733/136‑1 (MQ=255)                                                                       gTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATTAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGcc                                                        <  4:329050/135‑1 (MQ=255)                                                                          ccAGTGAAAAAGTACTGATTGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGACAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAgg                                                     >  5:188238/1‑135 (MQ=255)                                                                           cAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCACGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGc                                                    >  5:234666/1‑135 (MQ=255)                                                                           cAGTGAAAAAGTACTGAATGACTATATGAAAAGAGCAGATAAAGACTCGGATCAGAAGACGAGAGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGCATTCTGGCTCAACTGATCAAACCCGAGATGGCCAGGc                                                    >  1:364568/1‑135 (MQ=255)                                                                            aGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTg                                                  <  8:50591/136‑1 (MQ=255)                                                                            aGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTg                                                  <  8:57808/136‑1 (MQ=255)                                                                                aaaaaGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgc                                                                                                                             >  5:59959/1‑57 (MQ=255)                                                                                aaaaaGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgc                                                                                                                             <  6:59959/57‑1 (MQ=255)                                                                                  aaaGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATg                                            <  8:79725/136‑1 (MQ=255)                                                                                  aaaGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCACGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTAt                                             <  6:234666/135‑1 (MQ=255)                                                                                   taGTTCTGAATGACTATTTGATAATAGCAGATAAAGACTGGTTTCCTTAGCCGCGCGCTGGAGTGGATTGCGGAGCTTATAGCCAGGCGGATTGTGGGTGTTCTGATCTAACCCGAGATGGCCAGGCTGCGTATg                                            <  4:340545/134‑1 (MQ=255)                                                                                   aaGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATg                                            <  3:272553/135‑1 (MQ=255)                                                                                        ctgactgaCTATTTGAAAAGAGCAGATACAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGTCTGCCTATGCGCAg                                       <  7:312387/135‑1 (MQ=255)                                                                                        cTGAATTACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAg                                       <  8:437962/135‑1 (MQ=255)                                                                                         tgcaTGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAg                                       <  2:442154/131‑1 (MQ=255)                                                                                         tGAATGACTTTTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAg                                       <  4:5657/134‑1 (MQ=255)                                                                                         tGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGGGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAg                                       <  3:317332/134‑1 (MQ=255)                                                                                           aaTGACTATTAGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCa                                    >  4:302714/1‑135 (MQ=255)                                                                                             tGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAACCCGCGAGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTCTACTCATCAAACCCGAGATGACCAGGCTGCCTATGCGCAGGCAtt                                  >  5:301492/1‑135 (MQ=255)                                                                                              gACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTg                                 >  5:140671/1‑135 (MQ=255)                                                                                                   tttGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTtat                                                                                                   >  8:362044/1‑64 (MQ=255)                                                                                                   tttGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTtat                                                                                                   <  7:362044/64‑1 (MQ=255)                                                                                                   tttGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTg                            >  6:148376/1‑135 (MQ=255)                                                                                                         aaGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGAt                      <  6:339075/135‑1 (MQ=255)                                                                                                                gATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGt               <  1:8904/135‑1 (MQ=255)                                                                                                                gATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGt               <  1:259418/135‑1 (MQ=255)                                                                                                                gATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGt               <  5:66990/135‑1 (MQ=255)                                                                                                                              tCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCg                                                                                          >  3:152766/1‑46 (MQ=255)                                                                                                                              tCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCg                                                                                          <  4:152766/46‑1 (MQ=255)                                                                                                                              tCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGTGACGAGGAatat   >  5:280428/1‑133 (MQ=255)                                                                                                                              tCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGTGACGAGGAatat   >  3:374672/1‑133 (MQ=255)                                                                                                                              tCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGTGACGAGGAATATg  >  8:80896/1‑134 (MQ=255)                                                                                                                                   aGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGt               >  4:83473/1‑116 (MQ=255)                                                                                                                                   aGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGt               <  3:83473/116‑1 (MQ=255)                                                                                                                                       |                                                                                                                             GGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATTGCGGAGCTTATAGCCAGCCGGATTGTGGGTGTACTGATCAAACCCGAGATGGCCAGGCTGCCTATGCGCAGGCATTGCTCTGGGTGATCACGGGTGACGAGGAATATG  >  KT2440/1949496‑1949754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑