Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	140,311	A→C	19.8%	I358S (ATT→AGT)	CJ019_00149 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	140,311	0	A	C	19.8%	132.9 / 2.3	60	I358S (ATT→AGT)	CJ019_00149	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/10);  new base C (2/12);  total (38/22)
Fisher's exact test for biased strand distribution p-value = 2.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACC  >  KT2440/140180‑140439                                                                                                                                    |                                                                                                                                 ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAACTCg                                                                                                                               >  6:427503/1‑135 (MQ=255) ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCg                                                                                                                               >  4:87534/1‑135 (MQ=255) ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCg                                                                                                                               >  4:124969/1‑135 (MQ=255) ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCg                                                                                                                               >  5:93101/1‑135 (MQ=255) ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCg                                                                                                                               >  3:296180/1‑135 (MQ=255) ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGa                                                                                                                              >  8:250368/1‑136 (MQ=255) ttttCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGa                                                                                                                              >  7:424058/1‑136 (MQ=255)      aTGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGca                                                                                                                         >  8:402489/1‑136 (MQ=255)       tGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCCGCACGATGACCACAAAAATCGACTGca                                                                                                                         >  2:53240/1‑135 (MQ=255)       tGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTCGCCACTCACCACCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGcac                                                                                                                        >  8:53671/1‑136 (MQ=255)          accccgtGGCGGCGTATGGGGTTGCAGTTGCCGGGGGCTTCATTTCCCAAATTCTGGCAACTCCCCCCCCCATCACAATCACAAACAGCGCTCCCACCCCAACCACCACCATAACCACAAAAATCGACTGcacca                                                                                                                      <  4:369121/129‑1 (MQ=255)               cTGGCGGCCGACGCGGTTGCAGATGCGGCGGGCTTCGATTACGAAATTCTGCCCACTCACCCCCCCATCACCATGAAGACCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGcc                                                                                                                 <  4:296180/135‑1 (MQ=255)                  gcggcCGAAGGGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCCCCAGCCCATCTCCATGAAGAACAGCGCGGCCACCGCACCCAGCACGATGACCACAAAACTCGACTGCACCACTGCCGAg                                                                                                              <  2:130345/135‑1 (MQ=255)                  gcggcCGAAGCGGTTGCAGAGGCCGCGGGCTTCGATTACGCAATGCTGGCCACTCACCAGCCCATCTCCATGAAGAACCGCGCTGCCCCCGCATCCAGCACGATGACCACAAAACTCGACTGCACCACTGCCGa                                                                                                               <  7:32212/134‑1 (MQ=255)                      ccGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCAATCTCCATGAAGAACAGCGCTGCCACAGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAggtgg                                                                                                          >  3:27879/1‑135 (MQ=255)                         aaGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCCCTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCCAGGTGgtgt                                                                                                       >  1:431038/1‑135 (MQ=255)                         aaGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGCCCACAAAAATCGACTGCACCACTGCCGAGGTGgtgt                                                                                                       >  2:189017/1‑135 (MQ=255)                         aaGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGgtgtg                                                                                                      >  8:205564/1‑136 (MQ=255)                           ggggTTTCAGATGCCGGGGGCGTCGAATAGTAAATTCGGCCCCCTCCCCAGCCCATCTCCATGAAGACCGGCGCTGCCCCCCCATCCACAACTTTGCCCCAAAAACTCGACTGCACCACTGCCGAGGTGgtgt                                                                                                       <  6:343482/131‑1 (MQ=255)                            cGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGc                                                                                                   >  7:225874/1‑136 (MQ=255)                                          gcgGGCTTCTTATACGCATTTCTGCCCACCCACCCCCCAAACACCATCAACAACTGCCCGCCCACCCAAGCCACCCCGATCACCCCAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCgg                                                                                     <  7:201658/136‑1 (MQ=255)                                          gcgGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCAGGAAGAACAGCGCTGCCACCGCATCCCGCACGATGACCACAAAAATCGACTGCACCACTGCCGACGTGGTGTGTGCCCCCACCGACTCg                                                                                      >  6:247178/1‑135 (MQ=255)                                            gggCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGgc                                                                                    >  1:21351/1‑135 (MQ=255)                                            gggCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGgc                                                                                    >  3:54988/1‑135 (MQ=255)                                                ttagATTAAGCAATTCTGGCCACTCACCCGACCATCTCCAGGAAGACCAGCGCTACCACAGCATCAAGCACTATGACCACAAAAATAGACTCCACCACTGACGATGAGGTGTGTGAACCCACCGActctgctctg                                                                                >  4:260548/4‑135 (MQ=255)                                                   ttttACGCAATTGCGGCCAAACCCCAGCCATCCTCAATTAATAACCGCGCTCCCCGCGCACCCCCCCGGATCACCACAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGcc                                                                              <  4:27879/132‑1 (MQ=255)                                                          aaaTTCTGCCCACTCACCAGCCCATCCCCATGAAGAACCGCGCGGCCACCGCATCCAGCACTATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCtt                                                                     <  7:193464/135‑1 (MQ=255)                                                           aaTTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCtt                                                                     <  4:54988/135‑1 (MQ=255)                                                           aaTTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCt                                                                      >  5:250950/1‑134 (MQ=255)                                                           aaTTCTGGCACCTCCCCAGCCCATCTCAATGAAGAACCGCGCTGCCACCGGATCCAGCCCGTTGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCtt                                                                     <  3:213352/135‑1 (MQ=255)                                                           aaTTCTGCCACCTCACCCGCCCTTCACCATGAACAACTCCGCTGCCACCGCATCACCCACGATCACCAAAAACCTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCtt                                                                     <  6:345241/135‑1 (MQ=255)                                                                     aCTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGCCTCGGCGCTGCCGCTGCCCTTGACGCCtg                                                             >  4:152185/1‑132 (MQ=255)                                                                          ccAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGCCCTTGAAGCCTTCCAGGCa                                                      >  1:173878/1‑135 (MQ=255)                                                                          ccAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCa                                                      >  3:299863/1‑135 (MQ=255)                                                                          ccAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCa                                                      >  2:396008/1‑135 (MQ=255)                                                                          ccAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAg                                                     >  8:115193/1‑136 (MQ=255)                                                                          ccAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCACACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAg                                                     >  8:314041/1‑136 (MQ=255)                                                                          ccAGCCCATCTCCATGAAGAACAGCGCTGCCACAGCAACCAGCACGCTCCCCACAAACCTCCACTCCCCCACTCCCGAGGTGCTGTGTGCCCCCCCCGCCTCCGCGCTCCCGCTGCCCTAGCAGCCGTCCACGCAg                                                     >  8:112586/1‑136 (MQ=255)                                                                            aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGcc                                                   >  7:418827/1‑136 (MQ=255)                                                                                      cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATggcgg                                           >  2:371398/1‑134 (MQ=255)                                                                                          acgaaCCGCGCGGCCCCCGCACCCAGCACGATCACCACAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                      <  8:21038/133‑1 (MQ=255)                                                                                          aacaaCCGCGCGCCCACCGCACCCCCCACCATGCCCACAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                      <  3:104071/132‑1 (MQ=255)                                                                                           agaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAg                                    >  7:61402/1‑136 (MQ=255)                                                                                           agaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGAGCa                                     >  5:7011/1‑135 (MQ=255)                                                                                                aGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGCCCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGCTCAGGAAg                                >  6:311343/1‑135 (MQ=255)                                                                                                     tGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGcac                                                                                                                        >  8:303904/1‑42 (MQ=255)                                                                                                     tGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGcac                                                                                                                        <  7:303904/42‑1 (MQ=255)                                                                                                     tGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGCCCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGc                              >  5:404872/1‑132 (MQ=255)                                                                                                          ccGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACg                      >  2:258848/1‑135 (MQ=255)                                                                                                            gaacccAGAACGATCACAACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                    <  5:306294/131‑1 (MQ=255)                                                                                                            gCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                    <  6:397889/135‑1 (MQ=255)                                                                                                             cATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCCGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTg                   >  2:385211/1‑135 (MQ=255)                                                                                                                 cccaccGATGACCAAAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCtt               <  6:250950/130‑1 (MQ=255)                                                                                                                    cACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGc            <  1:371398/135‑1 (MQ=255)                                                                                                                     actattaCCACAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCa          <  7:53671/130‑1 (MQ=255)                                                                                                                     aCGATCACCACAAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCa          <  7:36146/136‑1 (MQ=255)                                                                                                                          cacaaaaaaaaTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGc       <  4:299863/128‑1 (MQ=255)                                                                                                                            ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGCCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTa    >  3:204764/1‑135 (MQ=255)                                                                                                                            ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAg                          >  6:297397/1‑113 (MQ=255)                                                                                                                            ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAg                          <  5:297397/113‑1 (MQ=255)                                                                                                                              caaaaacTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTAcc  <  2:431038/133‑1 (MQ=255)                                                                                                                              acaAAACTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTAcc  <  5:405968/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 TTTTCATGCACCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACC  >  KT2440/140180‑140439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑