Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,037,989	G→A	6.3%	S49N (AGC→AAC)	CJ019_01933 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,037,989	0	G	A	6.3%	200.5 / 2.5	63	S49N (AGC→AAC)	CJ019_01933	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/37);  new base A (2/2);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGCACGGTGTGCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTCGAACGC  >  KT2440/2037855‑2038119                                                                                                                                       |                                                                                                                                    caagcaCGGTGTGCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGc                                                                                                                                    >  7:15008/1‑136 (MQ=255)    gcaCGGTGTGCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑tt                                                                                                                                 <  6:333970/135‑1 (MQ=255)       gggTGTGAGGGCAAACCGGGGAATGGCCGGAAAAATTGCCGTGGGCTTGCCTTTGGTGCGGGTCCTGAGGCTGGGGGGGGCGGCCAGCGCGGTGGT‑GCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTc                                                                                                                            <  8:418174/135‑1 (MQ=255)         gtgtGCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTc                                                                                                                            <  5:28353/135‑1 (MQ=255)             gCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGTCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  7:438746/136‑1 (MQ=255)             gCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCt                                                                                                                        >  1:255342/1‑135 (MQ=255)             gCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCt                                                                                                                        >  4:33919/1‑135 (MQ=255)             gCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCt                                                                                                                        >  3:220070/1‑135 (MQ=255)             gCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  7:181183/136‑1 (MQ=255)              cTGGCCAACCTGGGCATGGCCCGCTAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  6:421562/135‑1 (MQ=255)              cTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  5:205035/135‑1 (MQ=255)              cTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  3:121993/135‑1 (MQ=255)              cTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  3:33919/135‑1 (MQ=255)               tGGCCTACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCTAAGAGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  2:323712/134‑1 (MQ=255)                     aCCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGtt                                                                                                               >  8:367926/1‑136 (MQ=255)                       cTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             <  8:254347/136‑1 (MQ=255)                        tGGGCATGGCCCGCAAACTTGGCGTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             <  3:127988/135‑1 (MQ=255)                        tGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             <  4:271112/135‑1 (MQ=255)                        tGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGGCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             <  4:17656/135‑1 (MQ=255)                         gggCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             >  1:337447/1‑134 (MQ=255)                         gggCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             >  5:97961/1‑134 (MQ=255)                              tGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             >  4:436265/1‑129 (MQ=255)                              tGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCa                                                                                                             <  3:436265/129‑1 (MQ=255)                              tGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCGTCGCCAGC‑GTGCGGCAGCGGGCCCAGGTCAATACCg                                                                                                       >  1:156022/1‑135 (MQ=255)                                gCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGAc                                                                                                     <  7:436950/135‑1 (MQ=255)                                       aaCTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCCGCGCTTCGACAAC‑GTGCGTCAGCTGGCCCAGTTCAATCCCGACTTACTgcg                                                                                             >  8:293361/1‑136 (MQ=255)                                       aaCTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAAC‑TTGCGTCACCTGGCCCAGTTCTATACCGCCTTACTgcg                                                                                             >  8:293354/1‑136 (MQ=255)                                             gCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCtt                                                                                          <  2:337447/133‑1 (MQ=255)                                              ccTGGGCGTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTgc                                                                                        <  1:400738/134‑1 (MQ=255)                                               cTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTTCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCCATACCGACTTACTGCGCATGcgcg                                                                                     >  7:132495/1‑136 (MQ=255)                                                tGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGcgcg                                                                                     <  5:128522/135‑1 (MQ=255)                                                     tttGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGGGGTGGAAAGCGTTTGCCCGCGCGTCGACAGCATTGCG‑CAGCTGGGCCAGGTGAAAACCGACGTACAGCGCGTgcgcccgcc                                                                                 >  5:114581/1‑129 (MQ=255)                                                     tttGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       >  4:370364/1‑96 (MQ=255)                                                     tttGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  3:370364/96‑1 (MQ=255)                                                     tttGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCAt                                                                                <  2:187741/135‑1 (MQ=255)                                                       tGCCTGGGTGCTGCGGCTGACGCGGGCGGGCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGGTGGCCAGCGCTTCGCCAGC‑TGGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCGTGCGCGAGCATGa                                                                              <  8:427895/135‑1 (MQ=255)                                                        gCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGa                                                                              <  2:255342/134‑1 (MQ=255)                                                        gCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGa                                                                              <  6:442260/134‑1 (MQ=255)                                                                gctgctgcgGACGCTGGCGGGCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGttt                                                                    <  7:189517/136‑1 (MQ=255)                                                                    ctgctgACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGcc                                                                 <  4:336808/135‑1 (MQ=255)                                                                      gctgACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCt                                                               >  3:84604/1‑135 (MQ=255)                                                                       ctgACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCCGCGCTTCGACAGC‑TTGCGTGAGCTGGCCCAGTTGTATACCGACTTTCTGCGCTTGCGCGAGGCTGAGCAGGCGTGTGcccgg                                                              >  6:345100/1‑133 (MQ=255)                                                                           cGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTgcg                                                            <  1:314590/133‑1 (MQ=255)                                                                            gCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTc                                                         >  2:74640/1‑135 (MQ=255)                                                                                   gTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATg                                                  <  5:162781/135‑1 (MQ=255)                                                                                          ccATCGGCGTCGTGGCGTTGCAAAGCGTTGGCGAGCGCTTCGACAAC‑TTGCGGCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGc                                          <  7:293354/136‑1 (MQ=255)                                                                                          ccAGCGGCGGCGGGGCGTTGCAAAGCGTTGGCCAGCGCTGCGACAAC‑TTGCGTCAGCTGGCCCAGGTCACTACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGc                                          <  7:293361/136‑1 (MQ=255)                                                                                               ggCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGAc                                      <  1:172049/135‑1 (MQ=255)                                                                                                       ggCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       >  4:316756/1‑46 (MQ=255)                                                                                                       ggCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTg                                                                                                                       <  3:316756/46‑1 (MQ=255)                                                                                                       ggCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCg                              >  4:387827/1‑135 (MQ=255)                                                                                                           gtGCAAAGCGGTGGCCAGCGCTTCGACAGC‑GGGCGTCAGCGGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTGGCCCGGCGCGCCGACCTGCAGCAGGCCGACGCTTTTCGTggc                          <  4:139431/134‑1 (MQ=255)                                                                                                                aaGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCt                     <  6:97961/135‑1 (MQ=255)                                                                                                                aaGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTg                    >  8:175197/1‑136 (MQ=255)                                                                                                                 aGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCCCCATGCAGCAGGCCGCCGCTTTTCGTGGCGGCCTg                    >  6:242104/1‑135 (MQ=255)                                                                                                                          cAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTgg          >  8:82002/1‑136 (MQ=255)                                                                                                                            gcgcTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTc        <  8:15008/136‑1 (MQ=255)                                                                                                                             cgcTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTc        <  3:301414/135‑1 (MQ=255)                                                                                                                             cgcTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTc        <  2:98187/135‑1 (MQ=255)                                                                                                                                ttCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCtt                                                                                          >  4:370598/1‑50 (MQ=255)                                                                                                                                ttCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCtt                                                                                          <  3:370598/50‑1 (MQ=255)                                                                                                                                ttCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTCGAAc    >  7:296701/1‑136 (MQ=255)                                                                                                                                   gACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTCGAAcgc  <  4:170359/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    CAAGCACGGTGTGCTGGCCAACCTGGGCATGGCCCGCAAACTTGGCCTGGGCTTTGCCTTGGTGCTGCTGCTGACGCTGGCGGTCGCGGCCATCGGCGTCGTGGCGTTGCAAAGCGTTGGCCAGCGCTTCGACAGC‑TTGCGTCAGCTGGCCCAGTTCAATACCGACTTACTGCGCTTGCGCGAGCATGAGCAGGCGTTTGCCCTGCGCTCCGACATGCAGCAGGCCGACGCTTTGCGTGGCGGCCTGCAGGGTTTGGTCGAACGC  >  KT2440/2037855‑2038119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑