Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,134,732	T→G	23.3%	L471V (TTG→GTG)	comEC →	ComE operon protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,134,732	0	T	G	23.3%	70.5 / 4.3	61	L471V (TTG→GTG)	comEC	ComE operon protein 3
Reads supporting (aligned to +/- strand):  ref base T (18/27);  new base G (11/5);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 7.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCCGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATCACAGCCTGCT  >  KT2440/2134599‑2134862                                                                                                                                      |                                                                                                                                   gTTCCGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCAGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTCCGCGGGtt                                                                                                                                   <  5:434465/135‑1 (MQ=255)  ttCCGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCGGCGCGGGGTg                                                                                                                                  >  3:313094/1‑135 (MQ=255)   tCCGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGtt                                                                                                                                   <  4:183899/133‑1 (MQ=255)     cGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGGTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGGGGGTTgggtg                                                                                                                              >  8:240549/1‑134 (MQ=255)       ggggCTGGCGCGGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAgg                                                                                                                             <  5:363824/134‑1 (MQ=255)          gCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAgggg                                                                                                                           >  3:102642/1‑134 (MQ=255)          gCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCGGCGCGGGGTGGGAGGGGt                                                                                                                          >  3:297712/1‑135 (MQ=255)               cgcTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGGTGCGCGGGTTGGGAGGGGTAAtgc                                                                                                                     >  1:76294/1‑135 (MQ=255)               cgcTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGGGGGGGTTGGGGGGGGTAAttc                                                                                                                     >  3:426332/1‑133 (MQ=255)               cgcTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCCGCCCTGCCGTTGTGGGCCTGGCTGCTGGGATGTCTGGGTGTGCTGTTGGTTTTTCTGCCCCGGGGTGTGCCGGTGCGGGGGTTGGGGGGGGTTAtggg                                                                                                                    >  8:91566/1‑134 (MQ=255)                gcTGGTGGCGCTGCAGCGCCCGGCATGGGGCCCGGCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGGGTGCTGTTGGTTTTGCTGCCCCGTGGGGTGCCGCTGCGCGGGTTGGGAGTGGTAAtgct                                                                                                                    <  2:218999/135‑1 (MQ=255)                gcTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAAtgct                                                                                                                    <  4:102642/135‑1 (MQ=255)                           agcagcGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGGTGCGCGGGGTGGGCGGGGTAATGCTGCTGGCGCggtg                                                                                                        >  8:272230/1‑136 (MQ=255)                            gcagcGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCtgtg                                                                                                        <  6:231097/135‑1 (MQ=255)                              agcGCCCGGCAGGGGGCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTGGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCtgtg                                                                                                        <  1:185161/133‑1 (MQ=255)                                 gcCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGcc                                                                                                  <  7:121319/136‑1 (MQ=255)                                  cccGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGcc                                                                                                  <  5:229165/135‑1 (MQ=255)                                   ccGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGGTGGGGGGGGGTATGCGGCGGGCGCGGTGGGGGcccc                                                                                                >  7:288394/1‑136 (MQ=255)                                     ggCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGGGCGGGTTGAGGGGGGTAATGGTGGTGGCGCGGTGGGTGGCCCgg                                                                                              >  7:60114/1‑136 (MQ=255)                                          gggTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGc                                                                                          <  5:172176/135‑1 (MQ=255)                                                cgccgGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGGTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGc                                                                                    >  4:104395/1‑135 (MQ=255)                                                  ccgGCCCTGCCGGTGTGGGCCTGGCTGCTGGTATGTGTGGGTGTGCTGTTGGTTTTGCGGCCCCGTGGTGTGCCGCGGGGCGGGGGGGGGGGGGGAATGCTGGTGGGGGTGTGGGTGCCCCGGGAGCCGGGGGCGt                                                                                 >  8:191979/1‑136 (MQ=255)                                                   cgGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCGGTGGGTGCCCCGGGCGCCGGTGCCGtt                                                                                >  8:269617/1‑136 (MQ=255)                                                        cTGCCGTTGTGGGCCAGGCTGCTGGTATGTCAGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCCCTGCCCGGGGTGGGTGGGGTTATGCTTCAGGCGCTGGGGGTGCCCCCGGCGCCGGTGCCCGTCGGcc                                                                           >  8:264126/1‑136 (MQ=255)                                                         tGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCACGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCgg                                                                             >  1:304276/1‑133 (MQ=255)                                                         tGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCGGTTGGTTTTGCTGCCCCGTGGTGTGGCGCGGCGCGGGTTGGGAGGGGTTATGCTGCGGGGGCGGTGGGTGCCCCCGGCGCCCGTGACGTGCgcgc                                                                           >  5:182534/1‑132 (MQ=255)                                                              ttGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTc                                                                      >  3:335761/1‑135 (MQ=255)                                                              ttGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCGGCGGGGGGTGGGTGGGGTTATGGTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTTCCGGTCGGGCCGGTc                                                                      >  5:135712/1‑135 (MQ=255)                                                              ttGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCGGCGCGGGGTGGGTGGGGTAATTCTGGGGGCGCTGTGGGTGCCCCCGGCGCCGGTGCCGGTCGGCCAGGTc                                                                      >  1:330725/1‑135 (MQ=255)                                                                    gCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTc                                                                <  4:229115/135‑1 (MQ=255)                                                                         gctgctGGTATGGCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGGTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCa                                                           <  6:423062/135‑1 (MQ=255)                                                                          ctgctgGTATGTCTGGGTGTGCTGTTGGGTTTGCTGCCCCGTGGTGGGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTGCGGCCAGGTCGAGGTCTGGCa                                                           <  4:192524/134‑1 (MQ=255)                                                                             ctgGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTgg                                                      <  7:74320/136‑1 (MQ=255)                                                                              tgGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTgg                                                      <  3:184829/135‑1 (MQ=255)                                                                                gTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGa                                                     <  8:449535/134‑1 (MQ=255)                                                                                     tCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTgg                                               <  4:335761/135‑1 (MQ=255)                                                                                     gcTGGGGGTGCTGTGGGTTTTGCGGCCCCGTGGTGTGCCGCTGCGCGGGGTGGGAGGGGGAATGCTGCTGGCGCTGGGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTgg                                               <  8:336033/134‑1 (MQ=255)                                                                                      cTGGGTGTGGTGTTGGTTTTGCTGCCCCTTGGTGGGCCGCTGCGCGGGTTTGGAGGGGTAATGCTGCTGGCGCGGTGGGGGCCCCGGGAGCCGGTGCCGTGCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTgg                                               <  3:119193/134‑1 (MQ=255)                                                                                      cTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTgg                                               <  6:135712/134‑1 (MQ=255)                                                                                      cTGGGTGTGCTGGTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTgg                                               <  5:11968/134‑1 (MQ=255)                                                                                        gggTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGCGCCGGGGCCGTTAGGCAAGGTCGCGGTCTGGCAGCGGGATGTTGGGCat                                           >  7:422574/1‑135 (MQ=255)                                                                                        gggTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCGTCGGGGGTTGGGAGGGGTAAAGCTGCTGGCGCTGTGGGTTCCCCGGGAGCCGCTGCCCTTCGGCCCGGTCGCGGTCTGGCAGCTGGCTGTTGGCCa                                            >  6:11968/1‑135 (MQ=255)                                                                                         ggTGTGCTGTTTGTTTTGCTGCCCCGGGGTGTGCCGCTGCGCGGGTTGGGAGGGGTTATGCTGCTGGCGATGTGGGTGCCCCGGGAGCCGGTGCCGGTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAgg                                          <  7:250952/136‑1 (MQ=255)                                                                                            gtgCTGGTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGc                                       <  8:295523/136‑1 (MQ=255)                                                                                             tgCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCt                                      >  8:23540/1‑136 (MQ=255)                                                                                             tgCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCGGCGCGGGTTGGGGGGGGTAATGCTGCTGGCGCGGTGGGTGCCCCCGGCGCCGGGGGCGGTCGGCCAGGTGGGGGGCTGGCCGCGGGGTGTTTGCCAGGGGc                                       >  2:148735/1‑135 (MQ=255)                                                                                               cTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTg                                     <  2:330725/135‑1 (MQ=255)                                                                                               cTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTg                                     <  3:104395/135‑1 (MQ=255)                                                                                               cTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGGTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTg                                     <  3:21466/135‑1 (MQ=255)                                                                                               cTGTTGGTTTTGCTGCCCCGTGGTGGGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTg                                     <  1:410822/135‑1 (MQ=255)                                                                                                  ttGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGGTCGGCCAGGTCGAGGTCTGGCCGCTGGATGTTTGCCAGGGGCTGGCg                                  >  5:119446/1‑135 (MQ=255)                                                                                                      ttttGCTGCGCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGGTGTGGGGTCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTg                               <  1:36931/134‑1 (MQ=255)                                                                                                               cccGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTAc                    <  8:288394/136‑1 (MQ=255)                                                                                                               cccGTGGTGTGCCCCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCGGTGGGTGCCCCGCGCGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGGAc                    >  8:306389/1‑136 (MQ=255)                                                                                                                      tgtgCCGCTGCGCGGGGTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGcc              >  5:180358/1‑134 (MQ=255)                                                                                                                          ccGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATcac          <  6:304430/135‑1 (MQ=255)                                                                                                                          ccGCTGCACGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATcac          <  2:304276/135‑1 (MQ=255)                                                                                                                           cGCAGCGCGGGTTGGGAGGGGTAAAGCTTCTTGCTCTGTGGGTGCCAAGAGAGCCGCTGCCGTTAGGCAAAGTAGAGGTCTTGCAGATTGATGTTTTCCAGGGGCTGGCGGTGCTCTATCGTACGCGGCaaaaaa         >  6:162942/1‑130 (MQ=255)                                                                                                                            gCTGCGCGGGGTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATCACAGc       <  7:332045/136‑1 (MQ=255)                                                                                                                             cTGCGCGGGTTGGGAGGTGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATCACAGc       <  3:4374/135‑1 (MQ=255)                                                                                                                             cTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATCACAGc       >  3:192759/1‑135 (MQ=255)                                                                                                                             cTGCGCGGGGTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGTCGGTGCCCTTGGGCCAGGTCGCGGTCTGGCAGCTGGATGTTGGGCAGGGGCTTGGGGGGCTCTTGCGGACGCGGCATcaccca       >  3:262124/1‑132 (MQ=255)                                                                                                                                  cgGGGTGGGAGGGGTACTGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATCACAGCCTGct  <  5:134012/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   GTTCCGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTATGTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGGGTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCGTACGCGGCATCACAGCCTGCT  >  KT2440/2134599‑2134862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑