| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,491,272 | A→C | 43.3% | H33P (CAC→CCC) | iutA → | Ferric aerobactin receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,491,272 | 0 | A | C | 43.3% | 15.0 / 7.5 | 31 | H33P (CAC→CCC) | iutA | Ferric aerobactin receptor |
| Reads supporting (aligned to +/- strand): ref base A (12/4); new base C (2/12); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCA > KT2440/2491161‑2491406 | cAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTTCAGTGCCGGGG‑CCC‑TGTTGCTCGGCCCCCTGCCCGCCGGGGCCGCGCCGGCTGCCgc > 8:313238/1‑135 (MQ=255) aGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGGTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCAGCCCCCAGGGGCCGCGACGGCGGCCGa > 4:134763/1‑135 (MQ=255) gCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATCTCTTGCAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACCGAc > 8:325247/1‑136 (MQ=255) cccgcggGCGGGGTTCTTTACCCCCGGCCTTGTTGTC‑TGCCGGGGCGGGG‑CCCGTGGTCCCCCCCCCCCGCCCCGCGGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGAcc < 1:110850/134‑1 (MQ=255) cggggcgGTTCTTTCCACCATGCCTTGATGTATTCAAGGGCCGGGG‑CCC‑TGTGGCGCGGCCCCCTGCCCGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAg < 7:357021/132‑1 (MQ=255) gTTCTTTTCCCCATGCCTTGAGCTCTTGCAGGGCCGGGC‑CCC‑TGTGGCTCGGCCCCCTCCACGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAAcc < 7:325247/136‑1 (MQ=255) aCCTTGCCTTGTTGGCTGGCCGTGCCGGGG‑CCC‑GGGTGCGCGCCCCCCGGCCCGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGAc < 4:210628/134‑1 (MQ=255) ccATGCCTTGTTGTGTCGCTG‑GCCGGGGCCCC‑TTTTGATCGCCCCCTACCCCGACGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAg < 8:95620/136‑1 (MQ=255) ccTTGTTGTCTTGCCGGGCGGGGG‑CCG‑TGTGCCGCGGCCCCCTCCACGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCt < 7:157538/136‑1 (MQ=255) ttGATGTCTTGCAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGCCGGCTGCCGAACAGCCCCAGATGCAGGCCCGCCAGGTCCCGCTCGCCCTGCCCGCCCCACCGCTGGCCCAGGCGCTg > 5:426433/1‑135 (MQ=255) ttGATGTCTTGCAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCCGGTCCAGCTCGCCCTGCCAGCCCCACCGCAGGACCAGgcgcgg > 5:283051/1‑133 (MQ=255) cAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGCCCAGGCGCTGACCACTTTCGc > 2:89087/1‑135 (MQ=255) cAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGCCCACGTTCGc > 3:422841/1‑135 (MQ=255) cAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGc > 3:221275/1‑135 (MQ=255) cAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGCCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCCGCCCAACCGCTGGACCAGCCGCTGACCACTTTCGc > 2:274196/1‑135 (MQ=255) cgggg‑CCG‑TGTTGCTCGGCCCCCTCCTCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCa < 5:345483/132‑1 (MQ=255) ccc‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccg > 4:106969/1‑135 (MQ=255) ccc‑TGTGGCGCGCCCCCCCCCCCGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccg < 3:114165/135‑1 (MQ=255) tGTTGCTCGGCCCCCTGCCGGCTGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggcc < 1:55231/135‑1 (MQ=255) gTTGCGCGGCCCCCTGCCCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTg < 7:443077/136‑1 (MQ=255) ttGCGCGGCCCCCTGCCCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTg < 6:347465/135‑1 (MQ=255) ggCTCGGCCCCGGCCCGGCGGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGc < 1:288263/134‑1 (MQ=255) cgcGGCCCCCGGCAGGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCAc < 4:180293/133‑1 (MQ=255) cTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGCCCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCAc > 2:109088/1‑135 (MQ=255) ggCCCCCTGCACGCCGGGGCCGCGACGGCTGCCGAACAGGCCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctg < 3:114628/135‑1 (MQ=255) gCCCCGCGCCCGCGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCCGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct < 8:197282/136‑1 (MQ=255) ccccgTGCCCGCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct < 1:389795/135‑1 (MQ=255) cccccTGCCCCCCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct < 6:334989/135‑1 (MQ=255) cccccTGCACGCAGGGGCCGCGCCGGCTGCCGAACAGACCCAGATGCAGGCCCGCCCGGTCCAGCTCGACCTGCCAGCCCCCCCGCTGGCCCCGGCGCTGCCCACTTTCGCCGACCAGGCCGGCCGGCCCcggcg > 3:17894/1‑134 (MQ=255) cccccTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCGCGCCCTGCCAGCCCCCCCGCGGGCCCCGGCGCGGACCACTTTCGCCGCCCAGGCCGGCCTGCCCcggct > 5:210628/1‑135 (MQ=255) cccTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCCGGTCCAGCTCGACCTGCCAGCCCACCCGCTGGACCAGGCGCGGCCCCCTTTCGCCGCCCCGGCCGGCCGGCACCGGCTGt > 2:389795/1‑135 (MQ=255) aCGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCACCCGCTGGACCAGGCGCTGCCCCCTATCGCCGCACCGGCCGGCCTGCACCGGCTGtggccca > 5:48080/1‑129 (MQ=255) | CAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGG‑CCC‑TGTTGCTCGGCCCCCTGCACGCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCA > KT2440/2491161‑2491406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |