Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,503,721 T→G 14.7% D356A (GAC→GCC)  copA_2 ← Copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,503,7210TG14.7% 112.7 / 2.1 66D356A (GAC→GCC) copA_2Copper resistance protein A
Reads supporting (aligned to +/- strand):  ref base T (23/31);  new base G (2/10);  total (25/41)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGGCGGCGTGGTCCATACCGGCCATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCACGTCG  >  KT2440/2503593‑2503849
                                                                                                                                |                                                                                                                                
aCATGGCGGCGTGGTCCATACCGGCCATGCTGCCGTGGTTCATGCCCTGCAGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCCTGCTCAGCCCCGGGCGCGGGGCggggg                                                                                                                            >  1:163330/1‑135 (MQ=255)
      cggcgTGGTCCATACCGGCCATGCGGCCGTGGTTCATGCCCTGCGGATTTCTGTCGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCAc                                                                                                                     <  7:367001/136‑1 (MQ=255)
       ggcgTGGTCCATACCGGCCATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCAc                                                                                                                     <  6:189291/135‑1 (MQ=255)
              tCCATACCGGCCATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCCGGCACCGGCGcc                                                                                                              >  6:364995/1‑135 (MQ=255)
                     cGGCCATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCGGCAGcc                                                                                                       <  4:38614/135‑1 (MQ=255)
                     cGGCCATGCGGCCGTGGTGCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGcc                                                                                                       <  6:241182/135‑1 (MQ=255)
                         cATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  <  7:117913/136‑1 (MQ=255)
                         cATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  <  7:141662/136‑1 (MQ=255)
                         cATGCTGCCGTGGTTCATGCCCTGCGGATTGCTGGGGTCCAGGCCCGCCCTGGCAGCCATGCCGCCGTGGCCCATGGCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  <  7:76423/136‑1 (MQ=255)
                         cATGCTGCCGTGGTGCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGGGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  <  7:113606/136‑1 (MQ=255)
                          aggctgcCGTGGTGCATGCCCTGCGGATTGCTGGGGGCCCTGCCCGCCATGGCATCCATGCCGCGGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCGGGcc                                                                                                  <  5:21039/133‑1 (MQ=255)
                          aggcggcCGTGGCGCAGGCCCGGCGGAGTGCGGTGGTCCAGGCCCGCCATGGCATCCATGCCGCCGGGGCCCATGGCTTCCATGCGCCGCACCGGGCGCGGGTCGGGGGCCGGCACCGGCGCCGGCAGCCCGGcc                                                                                                  <  1:412006/130‑1 (MQ=255)
                          aTGCTGCGGTGGTGCATGCCGTGCGGATTGCTGTGGTCCATGCCGGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  <  3:91007/135‑1 (MQ=255)
                          aTGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCCCCGGGCGCGGGTCGGGGGCCGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  >  5:195490/1‑135 (MQ=255)
                          aTGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCGCAGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGcc                                                                                                  <  6:206924/135‑1 (MQ=255)
                           tgctgcCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCCCCGGCGCCGGCAGCCCCGccc                                                                                                 >  2:386496/1‑134 (MQ=255)
                            gctgcCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCGCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGc                                                                                                <  4:181947/135‑1 (MQ=255)
                              tgcCGTGGTGCATGCCCTGCGGAGTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCGGCAGCCCGGCCGCAc                                                                                              <  6:224547/135‑1 (MQ=255)
                              ggcCGTGGTTCATGCCCTGCGGAGTTCTGGGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACg                                                                                             <  8:19434/135‑1 (MQ=255)
                               tccGTGGTGCATGCCCGGGGGATTTCTGTGGTCCAGGCCCGCCAGGGCAGCCATGCCGCCGGGGCCCAGGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACg                                                                                             <  2:373978/134‑1 (MQ=255)
                               gcCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACg                                                                                             >  2:155414/1‑135 (MQ=255)
                                 cggggTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACg                                                                                             <  4:65918/130‑1 (MQ=255)
                                         aTGCCCTGCGGATTTCTGTGGGCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCGGCAGCCCGGCCGCACGGGCCAGGGTa                                                                                   <  2:273134/135‑1 (MQ=255)
                                                   gATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCaa                                                                         <  3:145247/135‑1 (MQ=255)
                                                        cTGTGGGCCAGGCCCGCCCGGGCAGCCAGGCCGCCGGGGCCCATGGCTTCCATGCTCCGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCCCGGGCCAGGGTACCGCGGGCAAAGCCgg                                                                   <  7:349407/136‑1 (MQ=255)
                                                         tgtgGTCCATGCCCGCCATGGCAGCCATGCCGCCGGGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCGGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCg                                                                    <  4:375371/134‑1 (MQ=255)
                                                         tgtgGGCCATGCCCGCCATGGCAGCCATGCCGCCGGGGCCCATGTCTTCCATGCTCAGCACCGGGCGGGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCgg                                                                   <  4:198753/135‑1 (MQ=255)
                                                         tgtgGCCCAGGCCCGCCATGGCAGCCATGCCGCCGGGGCCCATGTCTTCCATGCTCCGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCgg                                                                   <  4:208628/135‑1 (MQ=255)
                                                            gggccATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCgg                                                                   <  6:361326/129‑1 (MQ=255)
                                                            ggTCCATCCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCgg                                                                   <  4:191353/132‑1 (MQ=255)
                                                                 aTGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCCCAGCCGGTGCGGTcc                                                           >  6:289371/1‑135 (MQ=255)
                                                                 aTGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTc                                                            >  1:330068/1‑134 (MQ=255)
                                                                            ggCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCCGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCGGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGc                                                <  4:159650/135‑1 (MQ=255)
                                                                            ggCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCGGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGc                                                <  1:17124/135‑1 (MQ=255)
                                                                             tcATCCATGCCGCGGGGGCCCTTGTCTTCCATGCTCCGCACCGGGGGCGGTTGGGGGGCTGGCACCGGCGCCTGCAGCCGGGCCGCACGGGCCAGGGTTCGGCGGGAAAAGCCGGTGCGGTCCATGCTCTGGGc                                                <  6:159290/133‑1 (MQ=255)
                                                                             gCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCCCGGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGa                                              <  7:404294/136‑1 (MQ=255)
                                                                             gCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGGGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGa                                              <  8:151799/136‑1 (MQ=255)
                                                                             gCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGa                                              <  8:402900/136‑1 (MQ=255)
                                                                              cATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGa                                              <  4:219091/135‑1 (MQ=255)
                                                                                    tGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCACAGCCGGTGCGGTCCATGCTCGGGGCGAACAggg                                        >  3:167323/1‑135 (MQ=255)
                                                                                      ccgccgTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCCCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGgtg                                      >  4:336943/1‑135 (MQ=255)
                                                                                        gccgTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTa                                    >  3:61273/1‑135 (MQ=255)
                                                                                          cgTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCCTGCTGTGGGGGCACAGGGTGGAgg                                  >  5:358060/1‑135 (MQ=255)
                                                                                          cgTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCAGCAGCCCGGCCGCACGGGCCAGGGTACCGCCGGCACAGCCGGTGCGGTGCATGCTCTGGGCGCACACGGGGTAgg                                  >  5:145676/1‑135 (MQ=255)
                                                                                               cccATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCAGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCGCTGGGCGAACAGGGGGTAGGCGGGt                             >  5:251338/1‑135 (MQ=255)
                                                                                                      cTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCCGCCCGGCCGCCCGGGCCAGGGTACCGCGGGCAAAGCCGGGGCGGTCCATGCTCTGGGCGCACAGGGTGTAGGCGGGTTGGTccc                      >  4:255547/1‑133 (MQ=255)
                                                                                                       ttCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCAccc                    >  7:118378/1‑136 (MQ=255)
                                                                                                          cATGCTCAGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCAccc                    <  4:230732/133‑1 (MQ=255)
                                                                                                           aTGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACg                 >  4:258847/1‑135 (MQ=255)
                                                                                                            tGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACgg                >  4:430754/1‑135 (MQ=255)
                                                                                                                 aGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCa          <  7:236382/136‑1 (MQ=255)
                                                                                                                 aGCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCGGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCa          <  8:36484/136‑1 (MQ=255)
                                                                                                                  gCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCa          <  3:198938/135‑1 (MQ=255)
                                                                                                                  gCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCa          <  3:258847/135‑1 (MQ=255)
                                                                                                                  gCACCGGGCGCGGGGCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCa          <  6:101086/135‑1 (MQ=255)
                                                                                                                    aCCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCa       >  8:213596/1‑136 (MQ=255)
                                                                                                                      cGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGa                                              >  7:445644/1‑95 (MQ=255)
                                                                                                                      cGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGa                                              <  8:445644/95‑1 (MQ=255)
                                                                                                                       gggCGGGGGTCGGGGGCCGCCACCGGCGCGGGCAGCCGGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCAcg     <  1:407928/135‑1 (MQ=255)
                                                                                                                       gggCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCAcg     <  6:195490/135‑1 (MQ=255)
                                                                                                                           gcgGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCAcgtcg  >  7:2709/1‑134 (MQ=255)
                                                                                                                           gcgGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCAcgtcg  >  7:11011/1‑134 (MQ=255)
                                                                                                                           gcgGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCAcgtc   >  1:376263/1‑133 (MQ=255)
                                                                                                                           gcgGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCAcgtc   >  1:343211/1‑133 (MQ=255)
                                                                                                                           gcgGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGACGGTGCGGTCCATGCTCGGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCCCCAGCAcgtc   >  6:248022/1‑133 (MQ=255)
                                                                                                                           gcgGGTCGGGGGCAGGCACCGGCGCCTGCAGACCGGCCGAACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCCCCAGCAcgtc   >  4:60104/1‑133 (MQ=255)
                                                                                                                                |                                                                                                                                
ACATGGCGGCGTGGTCCATACCGGCCATGCTGCCGTGGTTCATGCCCTGCGGATTTCTGTGGTCCATGCCCGCCATGGCATCCATGCCGCCGTGGCCCATGTCTTCCATGCTCAGCACCGGGCGCGGGTCGGGGGCAGGCACCGGCGCCTGCAGCCCGGCCGCACGGGCCAGGGTACCGCGGGCAAAGCCGGTGCGGTCCATGCTCTGGGCGAACAGGGTGTAGGCGGGTTGGTCACCCACGGTCACCAGCACGTCG  >  KT2440/2503593‑2503849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: