Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,514,700	C→T	5.9%	A965V (GCC→GTC)	CJ019_02369 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,514,700	0	C	T	5.9%	217.3 / 3.2	68	A965V (GCC→GTC)	CJ019_02369	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/33);  new base T (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01

TTCGAGCGCCTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATCGCATTACCCTG  >  KT2440/2514570‑2514833                                                                                                                                   |                                                                                                                                      ttCGAGCGCCTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGCCGGCCGACAGCATCCTGCGAGCCTgg                                                                                                                                   >  4:16620/1‑135 (MQ=255)        gcCTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACt                                                                                                                              >  6:83024/1‑133 (MQ=255)        gcCTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCa                                                                                                                           <  8:430554/136‑1 (MQ=255)        gcCTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTAACGGCCGACAGCATCCTGCGAGCCTGGCGACt                                                                                                                              >  1:214249/1‑133 (MQ=255)         ccTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCGGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCa                                                                                                                           <  5:156325/135‑1 (MQ=255)            ggTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCACCCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGc                                                                                                                       <  7:121239/136‑1 (MQ=255)            ggTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGc                                                                                                                       >  8:409232/1‑136 (MQ=255)            ggTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGAAGGAGGGGCGACGCGCCGCCAGCAGCTTGACGCCCGACAGCATCCTGCGAGCCTGGCGACTGCAggg                                                                                                                        >  1:418583/1‑135 (MQ=255)              tAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCg                                                                                                                      <  2:124192/135‑1 (MQ=255)                         gAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTcg                                                                                                           >  2:272562/1‑135 (MQ=255)                                   aCCAGCTAGTGCTGTACCTCAACCGCTGGGGCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAggg                                                                                                <  8:268104/136‑1 (MQ=255)                                    ccAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAggg                                                                                                <  4:373299/135‑1 (MQ=255)                                    ccAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAggg                                                                                                <  3:200229/135‑1 (MQ=255)                                    ccAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAgg                                                                                                 <  2:19466/134‑1 (MQ=255)                                        cTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCaaa                                                                                           <  7:149401/136‑1 (MQ=255)                                          aGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGCGCCTGTATCGGCCGGCGAGGGGCAAac                                                                                          >  5:376975/1‑135 (MQ=255)                                          aGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAac                                                                                          >  5:414224/1‑135 (MQ=255)                                          aGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAac                                                                                          >  4:115118/1‑135 (MQ=255)                                           gTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTCGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGcacaca                                                                                         >  3:244091/1‑135 (MQ=255)                                               tGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGCCGGCCGACAGCCTCCTGCGAGCCTGGCGACTGCAGGGCGCGCCTGTTTCGGCCGGCGAGGGGCACACACAgg                                                                                     >  3:344104/1‑135 (MQ=255)                                               tGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAgg                                                                                     >  3:115184/1‑135 (MQ=255)                                                 tACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAgggg                                                                                   >  5:118699/1‑135 (MQ=255)                                                    cTCACCCGGGGGGGCGGTGCCGTGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGCCGGCCGACAGCAGCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAg                                                                                <  5:62870/135‑1 (MQ=255)                                                    cTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGTCGACTCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAg                                                                                >  4:295487/1‑135 (MQ=255)                                                    cTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGCGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAg                                                                                >  6:55179/1‑135 (MQ=255)                                                    cTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAg                                                                                >  3:101876/1‑135 (MQ=255)                                                    cTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGA‑GGGCAAACCCAGGGGCcgcg                                                                              >  7:384402/1‑136 (MQ=255)                                                     tCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAgc                                                                               >  5:277482/1‑135 (MQ=255)                                                         ccGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGCGCCTGTTTCGGCCGGCGAGGGGCAACCACAGGGGCAGCGCtt                                                                           >  2:273780/1‑135 (MQ=255)                                                           gCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTc                                                                        >  8:137872/1‑136 (MQ=255)                                                              gggTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGTCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGAt                                                                     >  8:333709/1‑136 (MQ=255)                                                              gggTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGTCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGAt                                                                     >  8:339505/1‑136 (MQ=255)                                                              gggTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGa                                                                      >  5:291943/1‑135 (MQ=255)                                                               gggcGGTGCCGAGTTGCAGGCGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGAt                                                                     <  6:245389/132‑1 (MQ=255)                                                               ggTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGAt                                                                     <  2:38941/135‑1 (MQ=255)                                                               ggTCGGTGCCGAGTGGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGAt                                                                     <  1:192516/135‑1 (MQ=255)                                                                    gTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCg                                                                <  2:20712/135‑1 (MQ=255)                                                                       ccGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGtt                                                             >  5:64680/1‑135 (MQ=255)                                                                             tGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTcggccgg                                                                                                      <  5:355094/88‑1 (MQ=255)                                                                             tGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTcggccgg                                                                                                      >  6:355094/1‑88 (MQ=255)                                                                               cggtcggggCGAGGCGCCGCCCGCTGGTTGCCGGCCGACAGCAGCCGGCGCGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGA‑GGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTgcg                                                   <  8:384402/131‑1 (MQ=255)                                                                                cggaggGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTgcg                                                   <  7:293356/135‑1 (MQ=255)                                                                                aggtggGGCGACGCGCCGCCCGCAGGTGGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTgc                                                    <  2:211627/135‑1 (MQ=255)                                                                                aggaggGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCGGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTgcg                                                   <  8:183562/136‑1 (MQ=255)                                                                                 ggaggGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTgcg                                                   <  6:291943/135‑1 (MQ=255)                                                                                 ggaggGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCAGCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTgcg                                                   <  1:138499/135‑1 (MQ=255)                                                                                       ccGACGCGCCGCCCGCAGGTTGAGGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGGCGATGAGCGGTTTGAGTTTGCGCACACt                                             <  5:49697/134‑1 (MQ=255)                                                                                         gACGCGCCGCCCGCAGGTTGCCGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGCGCGGTTTGAGTTTGCGCACACTGc                                           >  6:57714/1‑135 (MQ=255)                                                                                               ccgcccgcAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCAc                                     <  6:277482/135‑1 (MQ=255)                                                                                                    cgcAGGTTGACGGCCGACAGCATCCTGCGAGTCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTAccgc                               <  7:339505/136‑1 (MQ=255)                                                                                                    cgcAGGTTGACGGCCGACAGCATCCTGCGAGTCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTAccgc                               <  7:333709/136‑1 (MQ=255)                                                                                                              cGGCCGACAGCATCCTGAGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGTGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCTCCGCATATCg                      <  2:23022/135‑1 (MQ=255)                                                                                                               ggCCGACAGCATCCTGCGAGCCTGGCGCCTGCAGGGCGAGCCTGGTTCGGTCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTGTGAGTTTGCGCACCCTGCCAGCACTACCGCCGCAGATCGa                     >  3:28008/1‑135 (MQ=255)                                                                                                               ggCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGa                     >  5:32109/1‑135 (MQ=255)                                                                                                                gCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGAt                    >  3:418937/1‑135 (MQ=255)                                                                                                                  cGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGAttt                  <  5:12616/135‑1 (MQ=255)                                                                                                                   gACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTc                 >  8:365207/1‑135 (MQ=255)                                                                                                                    aCAGCATCCTGCGAGCGTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCTAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCa               <  7:325701/136‑1 (MQ=255)                                                                                                                    aCAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCTAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCa               <  7:325697/136‑1 (MQ=255)                                                                                                                     cAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCa               <  5:83024/135‑1 (MQ=255)                                                                                                                       gcctCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGGTTCGGCCGGCGAGGTGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTCCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATc             <  2:356835/132‑1 (MQ=255)                                                                                                                       gCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATc             <  3:47231/135‑1 (MQ=255)                                                                                                                       gCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATc             <  2:349463/135‑1 (MQ=255)                                                                                                                       gCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATc             <  3:295487/135‑1 (MQ=255)                                                                                                                          tCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATCGCAt         <  8:23121/136‑1 (MQ=255)                                                                                                                              gCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACCCTGCCAGCACTACCGCCGCATATCGATTTCCATCGCATTAc      >  3:46866/1‑135 (MQ=255)                                                                                                                              gCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATCGCATTAcc     >  7:171838/1‑136 (MQ=255)                                                                                                                                  gCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATCGCATTACCctg  >  3:227877/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                      TTCGAGCGCCTGGTAGAACTGCAGGAAGCGCATGACCAGCTAGTGCTGTACCTCAACCGCTGGGTCGGTGCCGAGTTGCAGGAGGGGCGACGCGCCGCCCGCAGGTTGACGGCCGACAGCATCCTGCGAGCCTGGCGACTGCAGGGCGAGCCTGTTTCGGCCGGCGAGGGGCAAACACAGGGGCAGCGCTTGTCGATGAGCGGTTTGAGTTTGCGCACACTGCCAGCACTACCGCCGCATATCGATTTCCATCGCATTACCCTG  >  KT2440/2514570‑2514833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑