Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,612,443 C→T 5.5% intergenic (‑89/‑75) CJ019_02468 ← / → CJ019_02469 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,612,4430CT 5.5% 233.8 / 3.2 73intergenic (‑89/‑75)CJ019_02468/CJ019_02469hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/42);  new base T (2/2);  total (29/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

ATCCTGCAGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTG  >  KT2440/2612309‑2612577
                                                                                                                                      |                                                                                                                                      
aTCCTGCAGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGcccc                                                                                                                                        >  1:202095/1‑135 (MQ=255)
       aGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGgc                                                                                                                                   <  6:84882/133‑1 (MQ=255)
        gTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGcgcg                                                                                                                                >  2:256137/1‑135 (MQ=255)
         tttCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGt                                                                                                                               >  5:169245/1‑135 (MQ=255)
         tttCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTc                                                                                                                              >  8:33715/1‑136 (MQ=255)
             aaaGATCCCTTCAAAATCTTTCAAGTCGTTCAATTCGTTCCGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTg                                                                                                                           <  6:63581/135‑1 (MQ=255)
              aaGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTggg                                                                                                                         >  7:329433/1‑136 (MQ=255)
              aaGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTgg                                                                                                                          >  4:384675/1‑135 (MQ=255)
                      ttCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCt                                                                                                                            <  6:23539/125‑1 (MQ=255)
                      ttCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCt                                                                                                                            >  5:23539/1‑125 (MQ=255)
                      ttCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCtt                                                                                                                  >  5:330858/1‑135 (MQ=255)
                         aaaaGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGc                                                                                                                <  5:139965/134‑1 (MQ=255)
                          aaaGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCg                                                                                                               <  4:209952/134‑1 (MQ=255)
                          aaaGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCg                                                                                                               <  3:227481/134‑1 (MQ=255)
                          aaaGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCg                                                                                                               <  5:442195/134‑1 (MQ=255)
                                   aaGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTg                                                                                                    >  7:225784/1‑136 (MQ=255)
                                   aaGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCGGTGCCTTg                                                                                                    <  7:277990/136‑1 (MQ=255)
                                    aGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTg                                                                                                    <  5:194107/135‑1 (MQ=255)
                                    aGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTg                                                                                                    <  1:182948/135‑1 (MQ=255)
                                           cAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCt                                                                                                                            >  5:306449/1‑104 (MQ=255)
                                           cAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCt                                                                                                                            <  6:306449/104‑1 (MQ=255)
                                            aaTTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCTCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTcc                                                                                           >  8:198576/1‑136 (MQ=255)
                                            aaTTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCTCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTcc                                                                                           >  8:203884/1‑136 (MQ=255)
                                            aaTTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGgctcgctc                                                                                            >  2:379297/1‑135 (MQ=255)
                                             aTTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCt                                                                                          <  7:215140/136‑1 (MQ=255)
                                              ttCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTATCCTAGGCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCt                                                                                          <  2:115752/135‑1 (MQ=255)
                                              ttCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCt                                                                                          <  1:152554/135‑1 (MQ=255)
                                                 gTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTgc                                                                                        <  5:80925/134‑1 (MQ=255)
                                                   tACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGcgcg                                                                                     <  3:83139/135‑1 (MQ=255)
                                                         ccATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGcc                                                                                 >  1:442596/1‑133 (MQ=255)
                                                           aTTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCa                                                                               <  5:229472/133‑1 (MQ=255)
                                                           aTTTAGATAATGCATTTCCCTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGAAAACAACAGGAGCCCCCAGGCGCGTCCTGGTCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCaa                                                                              <  6:312995/134‑1 (MQ=255)
                                                                gTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCTCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  7:203884/136‑1 (MQ=255)
                                                                gTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCTCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  7:198576/136‑1 (MQ=255)
                                                                gTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  8:297908/136‑1 (MQ=255)
                                                                gTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  8:329433/136‑1 (MQ=255)
                                                                 tGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  2:202095/135‑1 (MQ=255)
                                                                 tGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  1:162332/135‑1 (MQ=255)
                                                                  gAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTcc                                                                       <  1:379297/134‑1 (MQ=255)
                                                                    aTGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCttt                                                                   >  7:339175/1‑136 (MQ=255)
                                                                    aTGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCt                                                                     <  6:431082/134‑1 (MQ=255)
                                                                    aTGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCt                                                                     <  4:87953/134‑1 (MQ=255)
                                                                    aTGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCt                                                                     <  1:256137/134‑1 (MQ=255)
                                                                       cATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGcc                                                                >  8:252426/1‑136 (MQ=255)
                                                                       cATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGc                                                                 >  5:81986/1‑135 (MQ=255)
                                                                       cATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGc                                                                 >  1:51566/1‑135 (MQ=255)
                                                                       cATTTCCGTAGGTAGCATAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGc                                                                 >  5:223202/1‑135 (MQ=255)
                                                                        aTTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTTCGGCTTTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGTAGTCCCGTTGcc                                                                >  5:353512/1‑135 (MQ=255)
                                                                        aTTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCAAGGCGCGTCCTGGGCGGCCTTGCGCCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCg                                                               >  7:92397/1‑136 (MQ=255)
                                                                              gtaggtagCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgac                                                         <  7:227524/136‑1 (MQ=255)
                                                                               taggtagCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgac                                                         <  2:81317/135‑1 (MQ=255)
                                                                                     gCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACcg                                                   >  2:345339/1‑135 (MQ=255)
                                                                                       cTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACcgcg                                                 >  2:157524/1‑135 (MQ=255)
                                                                                         aGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGCCCGCGt                                                >  4:54399/1‑134 (MQ=255)
                                                                                         aGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGt                                                >  3:79443/1‑134 (MQ=255)
                                                                                         aGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGt                                                >  2:410363/1‑134 (MQ=255)
                                                                                             tAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgact                                                        >  8:242634/1‑122 (MQ=255)
                                                                                             tAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgact                                                        <  7:242634/122‑1 (MQ=255)
                                                                                                ctgctAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCGTGCGGCGGTGCCGTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTc                                        <  1:354389/135‑1 (MQ=255)
                                                                                                 tgctAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCa                                       <  1:287844/135‑1 (MQ=255)
                                                                                                 tgctAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCa                                       <  6:330858/135‑1 (MQ=255)
                                                                                                   ctAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTg                                                                                                                           <  4:31593/49‑1 (MQ=255)
                                                                                                   ctAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTg                                                                                                                           >  3:31593/1‑49 (MQ=255)
                                                                                                   ctAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCAcc                                     >  2:49476/1‑135 (MQ=255)
                                                                                                    taataaAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCAcc                                     <  5:426283/134‑1 (MQ=255)
                                                                                                       taaAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGc                                 >  4:137211/1‑135 (MQ=255)
                                                                                                                  cTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACtt                     <  7:33715/136‑1 (MQ=255)
                                                                                                                     gTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTc                   <  2:325741/135‑1 (MQ=255)
                                                                                                                      tAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTc                   <  5:175658/134‑1 (MQ=255)
                                                                                                                            acaGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGtt            <  1:103157/135‑1 (MQ=255)
                                                                                                                            acaGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGAGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGtt            <  1:96827/135‑1 (MQ=255)
                                                                                                                              aGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCTGCTGTGCCTTGGCTCGCTCCTGCGCGGTCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTAt         <  7:300720/136‑1 (MQ=255)
                                                                                                                               ggTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATc        <  8:225784/136‑1 (MQ=255)
                                                                                                                                gTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATc        <  1:363578/135‑1 (MQ=255)
                                                                                                                                    ccccAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCt   <  7:171208/136‑1 (MQ=255)
                                                                                                                                      ccAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTg  >  1:4970/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ATCCTGCAGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCCTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTG  >  KT2440/2612309‑2612577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑