Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,663,206 G→C 5.2% A728A (GCC→GCG)  acnB ← Aconitate hydratase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,663,2060GC 5.2% 199.4 / 3.2 72A728A (GCC→GCG) acnBAconitate hydratase B
Reads supporting (aligned to +/- strand):  ref base G (30/36);  new base C (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

ATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTC  >  KT2440/2663075‑2663340
                                                                                                                                   |                                                                                                                                      
aTACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCa                                                                                                                                     <  5:76993/135‑1 (MQ=255)
 tACGTGCACCTGCTTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCAATCTAGGTTGTCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTATCCAGCATCTTgcaggcag                                                                                                                                    >  1:117606/1‑135 (MQ=255)
 tACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGCGCCAGCCACAGACGGGTCGGCATGCCCCCCTTGACCTTCTCCCGCAGCTTGCCGGCAgc                                                                                                                                   >  7:204800/1‑136 (MQ=255)
     tGCACCGGCCTTGCCGTATATGCCGTAGTAGCCTTCTACGGTCAGATGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGcgcg                                                                                                                                <  6:28505/135‑1 (MQ=255)
        aCCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAg                                                                                                                            >  8:202981/1‑136 (MQ=255)
          cGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGt                                                                                                                           >  7:59714/1‑135 (MQ=255)
           ggCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTg                                                                                                                          >  3:72806/1‑135 (MQ=255)
               ttGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCg                                                                                                                      <  6:408864/135‑1 (MQ=255)
               ttGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGa                                                                                                                     >  7:179788/1‑136 (MQ=255)
               ttGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGa                                                                                                                     >  8:347065/1‑136 (MQ=255)
                  ccGTAGATGCCGGAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGa                                                                                                                     <  6:642/133‑1 (MQ=255)
                    gTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGTAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCCGCGCGGAAGTGCCCGATGTTg                                                                                                                >  7:386696/1‑136 (MQ=255)
                    gTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTg                                                                                                                >  8:326831/1‑136 (MQ=255)
                          gCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGTCGGAGCCAGACACAGACGGGTCGGAATGCCACCCTTGACCTTCTCAAGCAGCTTTCCGGCAGCGCGGAAGAGAACGATGTTGCTCAt                                                                                                           >  5:334929/1‑135 (MQ=255)
                          gCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGCCCGATGTTGGTCAt                                                                                                           >  3:72630/1‑135 (MQ=255)
                          gCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCAt                                                                                                           <  5:130947/135‑1 (MQ=255)
                              tagtagCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCAc                                                                                                       <  1:303650/135‑1 (MQ=255)
                               agtagCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCAc                                                                                                       >  4:152576/1‑134 (MQ=255)
                               agtagCCTTCTTCGGTCAGCTGGTGAGCGTCAATCTTGGTTGGCGGAGACAGCAACAGACGGGTCGGAATGCCACCCTTGACCTTCTCAAGAAGATTGCCGGCAGCGCGTAAGTGACCGATTTTGTTCCTGCAc                                                                                                       >  1:315044/1‑134 (MQ=255)
                                      ttcttcTGTCAGATGGGTCTCGTCCATCTTGGTTGGCGGAGCCATCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGAt                                                                                               <  2:315044/135‑1 (MQ=255)
                                      ttcttcGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGAt                                                                                               <  6:210338/135‑1 (MQ=255)
                                         ttcGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGaa                                                                                            >  4:106103/1‑135 (MQ=255)
                                         ttcGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGaa                                                                                            >  1:406172/1‑135 (MQ=255)
                                           cGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAaca                                                                                          <  7:347065/135‑1 (MQ=255)
                                              tCAGCTGGTGAGCGTCCAGCTTGGGTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACtt                                                                                       <  6:267476/135‑1 (MQ=255)
                                                     gTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               <  8:223191/136‑1 (MQ=255)
                                                      tGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               <  3:184368/135‑1 (MQ=255)
                                                      tGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               <  4:211829/135‑1 (MQ=255)
                                                           gTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTtctc                                                                          <  1:131557/135‑1 (MQ=255)
                                                             ccATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGtt                                                                                                                 <  8:448597/94‑1 (MQ=255)
                                                             ccATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGtt                                                                                                                 >  7:448597/1‑94 (MQ=255)
                                                               aTCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGACCCGATGAACACTTCGTCGATCTTCTCGCCtt                                                                     >  8:201225/1‑136 (MQ=255)
                                                               aTCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCt                                                                      >  6:402127/1‑135 (MQ=255)
                                                                tacgggttggCGGTGCCACCCACAGGCGGGTCGGCAACTGACCTTTATGCGCATCCAGCAGTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGATCTTCTCAccct                                                                     <  3:279198/131‑3 (MQ=255)
                                                                  ttggttggCGGAGCCAGCCACAGACGGGTCGGAATGACACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGAGGAAGTGACCGATGTTGGTCATGAACGAAACGATGAACACTTCGTCGATCTTCTCGCCTTgg                                                                   >  5:213863/1‑134 (MQ=255)
                                                                      ttggCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTAcc                                                                <  3:381080/134‑1 (MQ=255)
                                                                      ttggCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTAcc                                                                <  4:409448/134‑1 (MQ=255)
                                                                        ggCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAg                                                             <  8:442545/135‑1 (MQ=255)
                                                                          cGGAGCCAGCCACAGACGGTTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCCCGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGa                                                           <  6:241424/135‑1 (MQ=255)
                                                                             agccagccACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGCCCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGAcag                                                        >  1:374510/1‑135 (MQ=255)
                                                                                  gccaCAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGGCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCg                                                  <  7:155578/136‑1 (MQ=255)
                                                                                   ccaCAGACGGGTCGGAATGCCTCCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGGCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCg                                                  <  1:178464/135‑1 (MQ=255)
                                                                                   ccaCAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCg                                                  <  3:152576/135‑1 (MQ=255)
                                                                                               cGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGtt                                                                                                                 >  7:425495/1‑60 (MQ=255)
                                                                                               cGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGtt                                                                                                                 <  8:425495/60‑1 (MQ=255)
                                                                                                ggAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGt                                    >  8:266764/1‑136 (MQ=255)
                                                                                                 gAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGt                                    >  6:322195/1‑135 (MQ=255)
                                                                                                    tGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGt                                 <  1:162681/135‑1 (MQ=255)
                                                                                                       cACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTc                               <  5:402127/134‑1 (MQ=255)
                                                                                                       cACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTc                               <  2:39600/134‑1 (MQ=255)
                                                                                                         cccTGGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCTAGGACAGCAGGCGGGCAGCGTCCGGGTCGTTCGgcg                           <  8:185230/136‑1 (MQ=255)
                                                                                                          ccTTGACCTTCTCGAGCAGCTTGCCGTCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAAcc                                                                                                  <  3:389406/64‑1 (MQ=255)
                                                                                                          ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGCACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTAGgcgc                          >  8:433907/1‑136 (MQ=255)
                                                                                                          ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               >  6:310708/1‑83 (MQ=255)
                                                                                                          ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               <  5:310708/83‑1 (MQ=255)
                                                                                                          ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                          <  7:202981/136‑1 (MQ=255)
                                                                                                          ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                          <  7:326831/136‑1 (MQ=255)
                                                                                                          ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCATGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                          >  8:201950/1‑136 (MQ=255)
                                                                                                          ccTTGACCTTCTCAAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAAcc                                                                                                  >  4:389406/1‑64 (MQ=255)
                                                                                                           cTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                          <  6:389873/135‑1 (MQ=255)
                                                                                                                ccTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCaga                     <  5:430633/135‑1 (MQ=255)
                                                                                                                 cTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCagag                    <  6:8549/135‑1 (MQ=255)
                                                                                                                    ctcCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCAGGGTCGTTCGGCGCGCAGAGca                  >  8:296850/1‑134 (MQ=255)
                                                                                                                      ccAGCAGCTTGCC‑GCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               >  4:66444/1‑70 (MQ=255)
                                                                                                                      ccAGCAGCTTGCC‑GCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                               <  3:66444/70‑1 (MQ=255)
                                                                                                                       cagcagTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGAtc                                                                               <  1:220461/70‑1 (MQ=255)
                                                                                                                       cagcagTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGAtc                                                                               >  2:220461/1‑70 (MQ=255)
                                                                                                                       cagcagTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGATCTTCTCACCCTGTACCGCAGACAGCGGACGCGCGTCATCCGGGTCGTTCGGAGCACacaggattgg              >  3:306128/1‑129 (MQ=255)
                                                                                                                              ttGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTg       <  6:334929/135‑1 (MQ=255)
                                                                                                                                gCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGGCGTTCGGCGCGCAGAGCACAGGCTCCTTGAc     >  2:430371/1‑135 (MQ=255)
                                                                                                                                  cGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTtcg                                                                                     >  5:251347/1‑53 (MQ=255)
                                                                                                                                  cGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTtcg                                                                                     <  6:251347/53‑1 (MQ=255)
                                                                                                                                  cGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGAAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGt   >  6:225780/1‑135 (MQ=255)
                                                                                                                                   ggCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTc  <  2:374510/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
ATACGTGCACCGGCCTTGCCGTAGATGCCGTAGTAGCCTTCTTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTC  >  KT2440/2663075‑2663340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑