Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,663,248	G→A	6.4%	F714F (TTC→TTT)	acnB ←	Aconitate hydratase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,663,248	0	G	A	6.4%	205.7 / 3.3	62	F714F (TTC→TTT)	acnB	Aconitate hydratase B
Reads supporting (aligned to +/- strand):  ref base G (23/35);  new base A (2/2);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

TTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGG  >  KT2440/2663116‑2663381                                                                                                                                     |                                                                                                                                      ttcGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGaa                                                                                                                                     >  4:106103/1‑135 (MQ=255) ttcGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGaa                                                                                                                                     >  1:406172/1‑135 (MQ=255)   cGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAaca                                                                                                                                   <  7:347065/135‑1 (MQ=255)      tCAGCTGGTGAGCGTCCAGCTTGGGTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACtt                                                                                                                                <  6:267476/135‑1 (MQ=255)             gTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        <  8:223191/136‑1 (MQ=255)              tGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        <  4:211829/135‑1 (MQ=255)              tGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        <  3:184368/135‑1 (MQ=255)                   gTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTtctc                                                                                                                   <  1:131557/135‑1 (MQ=255)                       aTCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGACCCGATGAACACTTCGTCGATCTTCTCGCCtt                                                                                                              >  8:201225/1‑136 (MQ=255)                       aTCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCt                                                                                                               >  6:402127/1‑135 (MQ=255)                        tacgggttggCGGTGCCACCCACAGGCGGGTCGGCAACTGACCTTTATGCGCATCCAGCAGTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGATCTTCTCAccct                                                                                                              <  3:279198/131‑3 (MQ=255)                          ttggttggCGGAGCCAGCCACAGACGGGTCGGAATGACACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGAGGAAGTGACCGATGTTGGTCATGAACGAAACGATGAACACTTCGTCGATCTTCTCGCCTTgg                                                                                                            >  5:213863/1‑134 (MQ=255)                              ttggCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTAcc                                                                                                         <  4:409448/134‑1 (MQ=255)                              ttggCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTAcc                                                                                                         <  3:381080/134‑1 (MQ=255)                                ggCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAg                                                                                                      <  8:442545/135‑1 (MQ=255)                                  cGGAGCCAGCCACAGACGGTTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCCCGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGa                                                                                                    <  6:241424/135‑1 (MQ=255)                                     agccagccACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGCCCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGAcag                                                                                                 >  1:374510/1‑135 (MQ=255)                                          gccaCAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGGCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCg                                                                                           <  7:155578/136‑1 (MQ=255)                                           ccaCAGACGGGTCGGAATGCCTCCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGGCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCg                                                                                           <  1:178464/135‑1 (MQ=255)                                           ccaCAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCg                                                                                           <  3:152576/135‑1 (MQ=255)                                                        ggAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGt                                                                             >  8:266764/1‑136 (MQ=255)                                                         gAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGt                                                                             >  6:322195/1‑135 (MQ=255)                                                            tGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGt                                                                          <  1:162681/135‑1 (MQ=255)                                                               cACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTc                                                                        <  2:39600/134‑1 (MQ=255)                                                               cACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTc                                                                        <  5:402127/134‑1 (MQ=255)                                                                 cccTGGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCTAGGACAGCAGGCGGGCAGCGTCCGGGTCGTTCGgcg                                                                    <  8:185230/136‑1 (MQ=255)                                                                  ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGCACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTAGgcgc                                                                   >  8:433907/1‑136 (MQ=255)                                                                  ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        >  6:310708/1‑83 (MQ=255)                                                                  ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        <  5:310708/83‑1 (MQ=255)                                                                  ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                                                                   <  7:326831/136‑1 (MQ=255)                                                                  ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                                                                   <  7:202981/136‑1 (MQ=255)                                                                  ccTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCATGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                                                                   >  8:201950/1‑136 (MQ=255)                                                                   cTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGgcgc                                                                   <  6:389873/135‑1 (MQ=255)                                                                        ccTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCaga                                                              <  5:430633/135‑1 (MQ=255)                                                                         cTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCagag                                                             <  6:8549/135‑1 (MQ=255)                                                                            ctcCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCAGGGTCGTTCGGCGCGCAGAGca                                                           >  8:296850/1‑134 (MQ=255)                                                                              ccAGCAGCTTGCC‑GCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        >  4:66444/1‑70 (MQ=255)                                                                              ccAGCAGCTTGCC‑GCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGAtc                                                                                                                        <  3:66444/70‑1 (MQ=255)                                                                               cagcagTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGAtc                                                                                                                        >  2:220461/1‑70 (MQ=255)                                                                               cagcagTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGAtc                                                                                                                        <  1:220461/70‑1 (MQ=255)                                                                               cagcagTTTACCCGCAGCACGGAAGTGACCGATGTTGGTCATGCAGGAACCGATAAACACTTCGTCGATCTTCTCACCCTGTACCGCAGACAGCGGACGCGCGTCATCCGGGTCGTTCGGAGCACacaggattgg                                                       >  3:306128/1‑129 (MQ=255)                                                                                      ttGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTg                                                <  6:334929/135‑1 (MQ=255)                                                                                        gCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGGCGTTCGGCGCGCAGAGCACAGGCTCCTTGAc                                              >  2:430371/1‑135 (MQ=255)                                                                                          cGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTtcg                                                                                                                              <  6:251347/53‑1 (MQ=255)                                                                                          cGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTtcg                                                                                                                              >  5:251347/1‑53 (MQ=255)                                                                                          cGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGAAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGt                                            >  6:225780/1‑135 (MQ=255)                                                                                           ggCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTc                                           <  2:374510/135‑1 (MQ=255)                                                                                                  cgGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAgg                                    <  2:406172/135‑1 (MQ=255)                                                                                                         gACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGAttt                             <  5:225780/135‑1 (MQ=255)                                                                                                          aCCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTc                            >  2:348590/1‑135 (MQ=255)                                                                                                             gATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGa                                                                                                    >  4:49123/1‑60 (MQ=255)                                                                                                             gATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGa                                                                                                    <  3:49123/60‑1 (MQ=255)                                                                                                             gATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCga                          >  4:420034/1‑134 (MQ=255)                                                                                                             gATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGCGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCga                          >  1:268349/1‑134 (MQ=255)                                                                                                                 ttGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCATGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGAtt                     <  7:201950/135‑1 (MQ=255)                                                                                                                      cATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCTTCGTCCGGGGCGTTCGGCGCGCAGAGCACAGGCTACTTGACGTCGGCCAGGTCGATTTCGATGATTTCgtc                <  3:258501/135‑3 (MQ=255)                                                                                                                            cGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTc          >  6:188351/1‑135 (MQ=255)                                                                                                                                  gATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGAtt                     >  7:124506/1‑118 (MQ=255)                                                                                                                                  gATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGAtt                     <  8:124506/118‑1 (MQ=255)                                                                                                                                  gATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATc    >  2:60538/1‑135 (MQ=255)                                                                                                                                   acgAACACTTCGTCGAGCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCgg  <  7:387664/134‑1 (MQ=255)                                                                                                                                    tGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGGCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCgg  <  1:76894/135‑1 (MQ=255)                                                                                                                                    tGAACACTTCGTCGAGCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGGCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCgg  <  4:156703/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TTCGGTCAGCTGGTGAGCGTCCATCTTGGTTGGCGGAGCCAGCCACAGACGGGTCGGAATGCCACCCTTGACCTTCTCCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGG  >  KT2440/2663116‑2663381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑