Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,954,385 A→C 21.5% V298G (GTG→GGG)  fatB ← Ferric‑anguibactin‑binding protein FatB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,954,3850AC21.5% 82.3 / 2.2 49V298G (GTG→GGG) fatBFerric‑anguibactin‑binding protein FatB
Reads supporting (aligned to +/- strand):  ref base A (24/13);  new base C (3/9);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 2.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGGCAGGCATTTGCAAAAAGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACATAGAGGATGTCGGGGTTGGCCTGCTGGATGA  >  KT2440/2954258‑2954520
                                                                                                                               |                                                                                                                                        
acagggcaggcaTTTGCAAAAAGGAAGTGCCTAGGCCTTATAGCCCCTCATCACGTCATCCGCTATCAACGCCAGTGAGGGCGGGCTGGCGGCCGTGACGAACCAGGCCTGCGGGTCGACGAACACCACGCGGcc                                                                                                                                   <  4:123560/131‑1 (MQ=255)
    ggcaggcaTTTGCAAAAAGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGc                                                                                                                               >  6:329604/1‑135 (MQ=255)
      caggcaTTTGCAAAAAGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCtt                                                                                                                             >  4:81306/1‑135 (MQ=255)
            tttGCAAAAAGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGc                                                                                                                       >  2:89724/1‑135 (MQ=255)
                cAAAAAGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTgg                                                                                                                  >  8:52117/1‑136 (MQ=255)
                  aaaaGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGtctgt                                                                                                                 >  3:123560/1‑131 (MQ=255)
                     cggACGTGCCTAGTCCTGATAGCCCCTGATCCCGTCATCCGCGATCAACCCCAGTGAGGGCGGGCGGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTg                                                                                                              <  3:115315/134‑1 (MQ=255)
                     agcacgTGCCTAGGCCTTATCGCCCCTGAGCACGTCAGCCGCGATCAAAGCCCGTGCGGGCGGGCGGGCGGCCGTGCCGTACCAGGCCGGCGGGGCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTg                                                                                                              <  2:145609/130‑1 (MQ=255)
                       gAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGgc                                                                                                            >  4:196081/1‑135 (MQ=255)
                             ccTAGTCCTGATACCCCCTTATCACGTCAACCGCCATCAACGCCTTTGCGGGCGGGGGGGCGGCCGTGACTTACCTGGCCGGCGGGTCGACTAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGcagcag                                                                                                     <  7:53959/136‑1 (MQ=255)
                              cttgcccTGATAGCCCCTCAGCCCGCCACCCGCCATCAACCCCTGGGCGGGCGGGGGGGCGGCCGTGACGTACCCGGCCGGGGGGGCGACTAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGcagcag                                                                                                     <  6:378104/130‑1 (MQ=255)
                              cTAGTCCTGATCCCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGGCGGGCTGGCGGCCGTGACGTACCAGGCCGGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGcagcag                                                                                                     <  3:230043/135‑1 (MQ=255)
                              cTAGTCCTGATACCCCCTGATCACGTCCTCCGCCACCAAAGCCTGTGCGGGCGGGCTGGCGGCCGTGACGTACCAGGCCGGCGGGGCGCCGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAgg                                                                                                    <  8:400925/136‑1 (MQ=255)
                                     tGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTg                                                                                              >  1:304166/1‑135 (MQ=255)
                                     tGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTg                                                                                              >  5:364079/1‑135 (MQ=255)
                                        tcgCCCCTGATCACGGCATCCGCGATCAAAGCCAGTGCGGGCGGGCGGGCGGCCGTGACGAACCTGGCCTGGGGGTCGACTAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTc                                                                                            <  4:144685/132‑1 (MQ=255)
                                          gCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAg                                                                                        >  7:388484/1‑136 (MQ=255)
                                          gCCCCTGAACACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGACTGGCGCAGCAGGGGGTTGTCCAAg                                                                                        >  8:239778/1‑136 (MQ=255)
                                            cccTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGGGACGTACCAGGCCTGCGGGGCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAgg                                                                                       <  1:247239/135‑1 (MQ=255)
                                               tGATCACGTCATCCGCGATCAAAGCCCGTGCGGGCGGGCGGGCGGCCGTGACGTACCCGGCCGGCGGGGCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGgtgt                                                                                    <  2:269898/135‑1 (MQ=255)
                                               tGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGgtgt                                                                                    >  3:111041/1‑135 (MQ=255)
                                               tGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGgtgt                                                                                    >  1:262446/1‑135 (MQ=255)
                                                gaacccGTCAGCCTCCATCAACGCCAGTGCGGGCGGGCGGGCGGCCGTTACGTACCAGGCCGGCGGGTCGCCGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTc                                                                                   <  4:157985/130‑1 (MQ=255)
                                                  tCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTctg                                                                                 >  4:432931/1‑135 (MQ=255)
                                                        cATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGtcat                                                                          >  8:82333/1‑136 (MQ=255)
                                                         aTCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGCCGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGGTGTCCAAGGTGTCTGCTGtca                                                                           >  1:323577/1‑134 (MQ=255)
                                                         aTCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGtcat                                                                          >  7:411704/1‑135 (MQ=255)
                                                         aTCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGtca                                                                           >  4:168420/1‑134 (MQ=255)
                                                                   accgCCAGTGAGGGCGGGCGGGCGGCCGTGCCGTACCCGGCCTGCGGGTCGCCGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCgg                                                                <  5:217640/132‑1 (MQ=255)
                                                                   aaaGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCgg                                                                >  1:259488/1‑135 (MQ=255)
                                                                        cAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGGTGGTCTGGCGCAGCAGGGGGTTGTACAAGGTGTCTGCTGTCTTAATTGGGCGGTGCt                                                            >  5:268048/1‑134 (MQ=255)
                                                                              ggTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑Acg                                                   >  7:237876/1‑136 (MQ=255)
                                                                              ggTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑Ac                                                    >  5:414192/1‑135 (MQ=255)
                                                                                  cggCTGGCGGCGGTGACGTACCGGGCGGGGGGGTGGACTAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑Acggcg                                                <  6:377276/134‑1 (MQ=255)
                                                                                        gcgggCGTGACGAACCTGGCCGGGGGGCCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGAt                                         <  8:308724/136‑1 (MQ=255)
                                                                                         gggccgggccgTCCCCGGCGGGGGGGGCGACCAACCCCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGAt                                         <  3:104809/134‑1 (MQ=255)
                                                                                         cggcCGTGACGTACCAGGCCTGCGGGGCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGAt                                         <  3:205268/135‑1 (MQ=255)
                                                                                              gTGACGTACCAGGCGGGGGGGGCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATccacc                                    <  3:177068/135‑1 (MQ=255)
                                                                                                gACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATccacccc                                  >  1:83675/1‑133 (MQ=255)
                                                                                                 aCGTACCCGGCCTGGGGGTCGACGAACACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACAt                                <  8:237876/136‑1 (MQ=255)
                                                                                                  cGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCACCCGGGGGTTGTCCAAGGTGTGTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGGGCGCTCccccacca                                >  5:377276/1‑133 (MQ=255)
                                                                                                  agaaCCAGGCGTGGGGGGCTACTAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACAt                                <  3:425242/132‑1 (MQ=255)
                                                                                                    tACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACATag                              <  3:196081/135‑1 (MQ=255)
                                                                                                       cAGGCGTGCGGCTCGACGACCACCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGGCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACATAGAGGa                          <  7:401880/136‑1 (MQ=255)
                                                                                                                 ggTCGACGAACACCACGCGGCCGTGGGTGCACGGGGTGGGCTGGCGCCGCAGGGGGTTTTGCCAGGGGGGTTGTGTTCTCATTTGGGGGTGCGCCATGTCCGGGGGTG‑GATCCCCCCCATATCGGATGTGggggg                 >  4:272471/1‑134 (MQ=255)
                                                                                                                 ggTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGAGCCACCACATAGAGGATGTCGGGGt                 >  5:14044/1‑135 (MQ=255)
                                                                                                                  gTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑CCGGCGGTGCGATCCACCACATAGAGGATGTCGGGGtt                >  2:156571/1‑135 (MQ=255)
                                                                                                                   tCGACGAACACCACGCGGCCGTGCTTCCCGGCGTTGGTCTGGCGCAGCAGGGGGTTGGCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑CCGGCGGTGCGATCCACCACATAGAGGATGTCGGGGTTgg              >  8:122281/1‑136 (MQ=255)
                                                                                                                     gacgacCCCCCCGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACATAGAGGATGTCGGGGTTGGcc            <  7:383788/136‑1 (MQ=255)
                                                                                                                               acGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACATAGAGGATGTCGGGGTTGGCCTGCTGGATGa  <  7:41103/136‑1 (MQ=255)
                                                                                                                               |                                                                                                                                        
CAGTGGCAGGCATTTGCAAAAAGGAAGTGCCTAGTCCTGATAGCCCCTGATCACGTCATCCGCGATCAAAGCCAGTGAGGTCGGGCTGGCGGCCGTGACGTACCAGGCCTGCGGGTCGACGAACACCACGCGGCCGTGCTTCCAGGCGTTGGTCTGGCGCAGCAGGGGGTTGTCCAAGGTGTCTGCTGTCATCATTGGGCGGTGCTCCATG‑ACGGCGGTGCGATCCACCACATAGAGGATGTCGGGGTTGGCCTGCTGGATGA  >  KT2440/2954258‑2954520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑