| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | 100% | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 97.8% | 122.2 / ‑6.6 | 41 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/9); minor base C (0/3); total (28/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT > KT2440/3061665‑3061924 | ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCTCTTTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGAGGCATGCTCTGGCCCTTCAAGTTGCCCAAGAACTTGCAGCAAACGGCAAACGTCaa > 5:52804/1‑135 (MQ=255) gTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc < 8:228925/136‑1 (MQ=255) tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAg > 3:198331/1‑134 (MQ=255) ccGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACCGGT‑CAAGGGCTTCAACCAGGGCGGCATGGTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACCGCACACGTCAAGCCATAAc > 1:44095/1‑135 (MQ=255) ggCTGGGTCGGCGCTGTGCCGCGGTGTGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGTTCGGCATGCTCTGGTCCTTGAAGTTTAAAAAGCTGTTGCAGATAACTGCAAACGTCAAGCCATAAct < 4:70707/134‑2 (MQ=255) ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgc > 1:140175/1‑135 (MQ=255) cTGGGGCGGCGCGGTGCCGCTGTGGGGCCGCGAAGTGGCCAAACGGGG‑CAACGACTTAAACCAAGGCGGCCTGCTCTGGACCTTCAACTTGCCCAAGCACTTTGAGCAAACGGCCAACCTCAACCCCAAACgccg > 5:54738/1‑134 (MQ=255) gggCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcctg‑c < 7:169762/134‑1 (MQ=255) cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGCCCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑c > 3:331524/1‑135 (MQ=255) gcgcGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCGCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTg < 3:274906/135‑1 (MQ=255) ggTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCt > 8:347800/1‑136 (MQ=255) gCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTtgctg < 3:344476/135‑1 (MQ=255) tgtgGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGcc < 5:182353/135‑1 (MQ=255) ggggCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCt < 4:278846/135‑1 (MQ=255) aGTGGCCAAACGGGT‑CATGGACTTCAACCTGGGCGCCATGCTCTGCACCTTCAAGTGCCCCACGCAGTTGCAGCAACCGGCAAACGCCACGACATAACGCCTC‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGGGGc < 8:209470/135‑1 (MQ=255) aGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTgg > 1:257781/1‑134 (MQ=255) gggCCAAACCGGTCCATGGATTTCAACCCGGGGGGATTGCTGTGCACATAAAATTGGCCCACCCTTTGGCAGAAACCGCAAATCGCCAAGCCATAACGCCTG‑CCTA‑CTGCCGATTGCTGCCGCTTAGCGGTgga < 2:12142/133‑2 (MQ=255) aaaCGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCGGATCGGTGGCAAGCGGc > 3:106451/1‑135 (MQ=255) aGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTAcc > 3:118221/1‑134 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCCGTGGCAAGCGGCCCTACTAACAAATGaag > 3:294299/1‑131 (MQ=255) cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCg > 3:177024/1‑135 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGCGCCCCGc > 6:50286/1‑135 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGc > 5:322105/1‑135 (MQ=255) gcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCGACTACCAAATGACGAGAGCCCCCCt > 3:16245/1‑135 (MQ=255) gcctctGCACCTTCAATTCCCCCACCCAGTTGCACCAAACGCCAAACGCCAAGCCA‑AACCCCCG‑CCCACCGGCCGCTGGCGGCCGCTGATCGGGGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGcc < 4:374002/133‑1 (MQ=255) gCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAAAGACGAGAGCCCCGCTGCCa > 2:395420/1‑135 (MQ=255) tGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGAt > 8:305600/1‑136 (MQ=255) aCCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCCACGATGCa > 7:403708/1‑136 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGCTGCATTCGcc > 3:396501/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcc > 4:90249/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCAACTACCAAATGACCAGAGCCCCCCAGCCAACCATGCAATCGcc > 2:207812/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCGGATCGGTGGCCAGCGGCCCTACTACCAAATGACGAGCGCCCCGCTGCCACCCCTGCATTAGcc > 6:27091/1‑135 (MQ=255) gTTGCCCAAGCAGATGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATAGGTGGCACGCGGCCCAACTAACAAAAGACGAGAGCCCCGCTGCCAACGCAGCATTCGccc > 6:243775/1‑135 (MQ=255) aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCCAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgc > 4:69763/1‑135 (MQ=255) aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg > 8:241366/1‑136 (MQ=255) cgCAGTTGCCGCAAACGCAAACCGACACCCCAAACCCCCTG‑CCTA‑CTGCCGCTGGCTGCCGCTGATCGGGGCCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg < 4:198331/134‑1 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTCGCAAGCGGCCCTACGACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCCCGCGGGGCTTCTTa > 8:258397/1‑136 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCGGAGCGGTGGCAAGCGGCCCTACTACCAAATGACGAGCGCCCCGCTGCCAACGATGCCTTCGCCCGGCACGCGGGGcttctt > 3:19152/1‑135 (MQ=255) ccaAACGGCAACCGGCAAGCCCTACCGCCTG‑CCAA‑CTGCCGCTTGCGGCCGCGGAGCGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc < 8:372339/135‑1 (MQ=255) caAACCCCAACCGTCACCCCAT‑ACGCCCGCCCAA‑CGGCCGCGTGCTGCCGCGGAGCGGGGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc < 1:270947/135‑1 (MQ=255) cAAACGTCAAGCCATAACGCCTG‑ACTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCCACCATGCATTCGCACGGCACGCGGGGCTTCTTAACaaagga > 6:101393/1‑130 (MQ=255) | GGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGT‑CAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTG‑CCTA‑CTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT > KT2440/3061665‑3061924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |