| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,130,135 | C→T | 6.7% | R94C (CGC→TGC) | livF_3 → | High‑affinity branched‑chain amino acidtransport ATP‑binding protein LivF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,130,135 | 0 | C | T | 6.7% | 183.2 / 3.6 | 60 | R94C (CGC→TGC) | livF_3 | High‑affinity branched‑chain amino acidtransport ATP‑binding protein LivF |
| Reads supporting (aligned to +/- strand): ref base C (29/27); new base T (2/2); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
GCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTGGAGCG > KT2440/3130000‑3130262 | gcCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCAc < 8:347293/136‑1 (MQ=255) ccAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCAc < 6:337994/135‑1 (MQ=255) ccAACGGGGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCAAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCAc < 6:209745/135‑1 (MQ=255) gCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGCCACCGCCGGTGTCGCGGCCCACCTGCTGGCCCGCCAGGGGATCg > 5:341665/1‑135 (MQ=255) actcgCACCACGGTCATGGCTTTTGCCGGCGTGGTGCCGGCCGACGGGGCGCATGTCAGTCGTGGCAATATCCGCTTCCTGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTc < 5:271112/131‑1 (MQ=255) aaGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTc < 3:72200/135‑1 (MQ=255) agagCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCCCGCCAGGGTATCGt > 1:227844/1‑133 (MQ=255) gCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACCCCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTgg > 7:87590/1‑134 (MQ=255) gCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTg > 3:88572/1‑133 (MQ=255) cTCAAGGCTATTTCCGGCCTGGTTCAGGCCGATCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGaa < 3:253360/135‑1 (MQ=255) tCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAgg > 8:225370/1‑136 (MQ=255) aaGGCTATTTCGGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGAc < 8:347618/136‑1 (MQ=255) gCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACg > 2:276337/1‑134 (MQ=255) ttCCGGCCTGGTTCAGGCCGACCGGGCGCAGGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGATGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCAtga > 6:274388/1‑134 (MQ=255) ccGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCCCGCCAGGGTATCGTCCATGTGTTGGTAGGACGGCATGTGtt > 7:211135/1‑136 (MQ=255) ccGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCAc < 7:291803/96‑1 (MQ=255) ccGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCAc > 8:291803/1‑96 (MQ=255) ccGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACATGCTGGCACGCCACGGTATCGTCCATGTGTTGGAAGGACCGCATgtgt > 6:434383/1‑135 (MQ=255) cGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTtc < 8:28266/136‑1 (MQ=255) tGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTccc > 6:216092/1‑135 (MQ=255) ggCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGAc < 2:97070/135‑1 (MQ=255) gACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCg < 7:225370/136‑1 (MQ=255) aCCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCg < 3:442129/135‑1 (MQ=255) aCCGGGCGAATGTCTGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCg < 1:257158/135‑1 (MQ=255) gggCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGGCGGCATGTGTTCTCCCACCTGCCTATCGAg > 4:112695/1‑134 (MQ=255) aTGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAAcc > 1:270175/1‑135 (MQ=255) cAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTgc > 6:122715/1‑134 (MQ=255) gTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTg > 6:145611/1‑135 (MQ=255) ccATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGACAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGCCTATCGAGGAAAACCTGCGCAGTgtcgg > 2:299541/1‑135 (MQ=255) ccATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAAACTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTggcggc > 7:29236/1‑136 (MQ=255) cATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTggcggc < 3:29406/135‑1 (MQ=255) ccGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCt < 4:88572/135‑1 (MQ=255) ccGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCt < 2:124854/135‑1 (MQ=255) cGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTg > 1:162868/1‑135 (MQ=255) ccAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAAc > 8:253736/1‑136 (MQ=255) ccAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCCTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTaa > 1:285726/1‑135 (MQ=255) ccAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCAGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACTGCATGTGTTCTCACACCTGACTCTCGAGGAAAACCTGCGCAGTGGCGGCATCCTGcttca > 6:29141/1‑131 (MQ=255) ccAGGGGCAGGACACCGCCGGTGTCGCGGACAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTaa > 2:41629/1‑135 (MQ=255) cAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGAGCAGTGGCGGCTTCCTGCGTAAc > 6:221978/1‑135 (MQ=255) cAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACATGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAAc > 4:352977/1‑135 (MQ=255) ggggCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCg < 8:251549/136‑1 (MQ=255) ggACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGAcgcg < 2:353608/135‑1 (MQ=255) gACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACgcgg > 3:52066/1‑135 (MQ=255) acacCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACgcggc > 2:3514/1‑135 (MQ=255) acacCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCAATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACgcggc > 1:70745/1‑135 (MQ=255) acCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTACCCCGACGCGgcgc > 7:264804/1‑135 (MQ=255) cgGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACt > 4:3511/1‑135 (MQ=255) cgGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACt > 5:398120/1‑135 (MQ=255) gtgtCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTgg < 5:395767/135‑1 (MQ=255) gtgtCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCCAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTgg < 3:78580/135‑1 (MQ=255) gtgtCGCGGCCAACCTGCGGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTgg < 5:145611/135‑1 (MQ=255) gggtCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTCTCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTgg < 3:152516/133‑1 (MQ=255) gggtCGCGGCCAACCTGCTGGCACGCCAGGGTAGCGGCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCGGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTgg < 3:84781/133‑1 (MQ=255) tgtCGCGGCCAACCTGATGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACTGCATGTGTCCTCCCACCTGACTCTCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGa < 6:25434/135‑1 (MQ=255) cgGCCAACCTGCTGGCATGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCAGCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGcc < 3:347062/123‑1 (MQ=255) cgGCCAACCTGCTGGCATGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGcc < 3:352990/123‑1 (MQ=255) cgGCCAACCTGCTGGCATGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGcc > 4:347062/1‑123 (MQ=255) cgGCCAACCTGCTGGCATGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGcc > 4:352990/1‑123 (MQ=255) aaCCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTgg < 8:87590/136‑1 (MQ=255) aaCCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTg < 3:21507/135‑1 (MQ=255) aCCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTgg < 1:276337/135‑1 (MQ=255) ccTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTGGa < 2:270175/135‑1 (MQ=255) ccTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTGGa < 6:429216/135‑1 (MQ=255) ccTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTGGa < 5:340561/135‑1 (MQ=255) tgGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTGGAgcg < 6:341665/133‑1 (MQ=255) | GCCAACGGTGCTGGCAAGAGCACCACGCTCAAGGCTATTTCCGGCCTGGTTCAGGCCGACCGGGCGCATGTCAGTCGTGGCCATATCCGCTTCCAGGGGCAGGACACCGCCGGTGTCGCGGCCAACCTGCTGGCACGCCAGGGTATCGTCCATGTGTTGGAAGGACGGCATGTGTTCTCCCACCTGACTATCGAGGAAAACCTGCGCAGTGGCGGCTTCCTGCGTAACCCGACGCGGCGCCAACTGGAGCAGGATCTGGAGCG > KT2440/3130000‑3130262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |