Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,202,757	A→T	21.0%	V308V (GTA→GTT)	hcf136_2 →	Ycf48‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,202,757	0	A	T	21.0%	70.0 / 4.5	41	V308V (GTA→GTT)	hcf136_2	Ycf48‑like protein
Reads supporting (aligned to +/- strand):  ref base A (4/27);  new base T (5/4);  total (9/32)
Fisher's exact test for biased strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAC  >  KT2440/3202625‑3202882                                                                                                                                     |                                                                                                                              tcGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCGTTCACCGACGGCGGCGGTGTTGAACGCCCAAGGTGGACAGGTATTg                                                                                                                            >  7:254458/1‑136 (MQ=255)         tGGCGGGCGGGCTGGATGGTCTTCGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGCAAAGGATGACCGCGGATGGCCTTTCACTGCCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCAc                                                                                                                     <  5:407759/135‑1 (MQ=255)          ggCGGTCGTGCTGTATGTGCTTAGCGTGCAGAGCTGCGATGGCGGTCACAGTTTCCAGGAAACGCATACCGCGGATGGCCTTTCACTTACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGATTTGTTTTCACg                                                                                                                    <  6:373102/135‑1 (MQ=255)          ggCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGc                                                                                                                   <  7:47350/136‑1 (MQ=255)           gCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTAAACGCCGAAGGTGTACCTGTATTGTTTTAACGc                                                                                                                   >  2:61266/1‑135 (MQ=255)                 gggCGGGAGGGGATTCGCGTGCAGAGCCGCTAGGGCGGGCACAGTTACCCGTAACCGCAGACCGCGGAGGGCCTTGCACTGCCGGCGGGGGTGTTCAAGGCCTACGGGGTACGTGTTTTGTTTTCACGCCGAggg                                                                                                             <  6:426089/135‑1 (MQ=255)                   gCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTTGAACGCCGAAGGTGGACCTGTTTTGTTTTCAGGCCGAggg                                                                                                             >  4:326172/1‑133 (MQ=255)                    cTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTaa                                                                                                         <  8:349066/136‑1 (MQ=255)                     tGGTTGGGCTTAGCGTGCAGAGCTGCGAGGGCGGCCACTGTTTCCAGTAACCGCAGACCGCGTTGGGCCTTTCAGTGCCGGCGGGGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTaa                                                                                                         <  5:419304/135‑1 (MQ=255)                     tGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGTTGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTaa                                                                                                         <  3:187653/135‑1 (MQ=255)                     tGGAGGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAAt                                                                                                        <  8:16428/136‑1 (MQ=255)                        aTGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCAATGACGGCCGCGTTGTTTAAACCCCAATGTGTTACTGTTTTTTTTTAAAGCCCACGGGTAAtta                                                                                                      >  3:45493/1‑133 (MQ=255)                          gggCTTAGCGGGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGACACGCAGACCGCGTATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTTTTGTTTTCACGCCGAGGGGTAATGccc                                                                                                    <  1:243701/135‑1 (MQ=255)                                aGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTTGAACCCCGAAGGGGTTCCTGTTTTGTTTTTCCGCCGAGGGGTTATTCCCTACCAg                                                                                              >  5:165246/1‑135 (MQ=255)                                     gcagcgcagcGATGGCGTGCACAGTTTCCAGGAAACGCAGACCGCGGTTGGCCTTGCACTTACGTCGGCGTTGTTCAACGCCTAATGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTg                                                                                         <  2:38645/135‑1 (MQ=255)                                         agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAggg                                                                                    >  8:125198/1‑136 (MQ=255)                                         agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTTGAAAGCCGCAGGTGTTCCTGGTTTTTTTTTACGCCGCGGGGTAATGCCCGACCAGGACTGAAgg                                                                                     >  5:427102/1‑135 (MQ=255)                                                              ttCCAGCAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGGGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGc                                                                <  4:62731/135‑1 (MQ=255)                                                              tcccAGTAAACGCAGACCGCGGTGGGCCTTTCACTGCCGGCGGCGTTGTTCACCGCCGAAGGTGTACCTTTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGaa                                                                  <  5:282552/131‑1 (MQ=255)                                                               tcaaGGAAACGCAGACCGCGGATGGCATTTCACTGACGGCGGCGTTGTTCAACGCGGAAGGTGTTCCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGcc                                                               <  8:254458/132‑1 (MQ=255)                                                               tCCAGGAAACGCAGCCCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGcc                                                               <  7:371108/135‑1 (MQ=255)                                                                cccggACACGCCGACCGCGGATGGCCTTACACTGACGGCGGCGTTTGTCAGCGCGGTTGGTGTACGTGTTTTGTTTGCACGCCGATGGGTAATGCACTACCTTGAGTGATGGGTTGGCCACTCGCTCAGAAGca                                                               <  2:400077/131‑2 (MQ=255)                                                                ccAGGAACCGCAGACGGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACGTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGcc                                                               <  1:61266/134‑1 (MQ=255)                                                                      accGCCGACCGCGTATGGCCTTTCACTGCCGGCGGCGTTGTCCACCGCCGATGGTGTACCTTTATTGTGTCCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATc                                                        <  1:439043/133‑1 (MQ=255)                                                                      aaCGCAGACCGGGGATGGCCTTTCACTGCCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATc                                                        <  1:44407/135‑1 (MQ=255)                                                                          ccgcccGCGGAGGGCCTTTCCCTGCCGGCGGCGTGGGCCAACGCCGAAGGTGGCCCTGTCTGGTTTGCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCaa                                                    <  5:274593/131‑1 (MQ=255)                                                                          cAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTAAACCCCGATGGTGTAGCTGGTTTGTGTTGAAGCCGCGCGGGAATTCCCTTCCAAGACTGCAGGGTTGGCCCCTCGCGCAGACGCCCCGCCTCGcca                                                    >  2:71204/1‑133 (MQ=255)                                                                              ccGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTTTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGa                                               <  8:234865/136‑1 (MQ=255)                                                                                    aTGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcct                                          <  1:286604/135‑1 (MQ=255)                                                                                      ggCCTTTCACTGCCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctgg                                        <  2:126915/135‑1 (MQ=255)                                                                                                   cggcggCGTTGTTCAACGCCTAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCtt                             <  4:437420/133‑1 (MQ=255)                                                                                                                 aaCGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggtg            <  7:125198/136‑1 (MQ=255)                                                                                                                  ccGCCGAAGGTGAACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGACATCGCAAAACGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggtg            <  5:66784/134‑1 (MQ=255)                                                                                                                      cGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGGGAATGGGTGGTCg        >  1:41737/1‑135 (MQ=255)                                                                                                                      cGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt       <  8:58732/136‑1 (MQ=255)                                                                                                                       taaGGTGTACCTTTATTGTTTTCACTCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt       <  2:321925/134‑1 (MQ=255)                                                                                                                       gatggTGTACCTGTATTGTTTACACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt       <  3:245300/132‑1 (MQ=255)                                                                                                                       gAAGGTGAACCTTTATTGTATGCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt       <  2:368922/135‑1 (MQ=255)                                                                                                                            tgtACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAc  <  6:427102/135‑1 (MQ=255)                                                                                                                            tgtACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAc  <  6:427935/135‑1 (MQ=255)                                                                                                                            tgtACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAc  <  4:423757/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                              TCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAC  >  KT2440/3202625‑3202882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑