Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,267,810 T→G 34.3% L171V (TTG→GTG)  eamA → putative amino‑acid metabolite efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,267,8100TG34.3% 37.5 / 5.5 30L171V (TTG→GTG) eamAputative amino‑acid metabolite efflux pump
Reads supporting (aligned to +/- strand):  ref base T (2/17);  new base G (9/2);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 1.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCC  >  KT2440/3267682‑3267930
                                                                                                                                |                                                                                                                        
cACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGt                                                                                                                      <  6:411475/133‑1 (MQ=255)
        ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGCCCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGGGGGGATCGGGGGt                                                                                                            >  6:314539/1‑135 (MQ=255)
             cGAAGCCGGCGGCCATGTGCCCATGCTGGCGTTGGTGCGGACCCTGTGGGGCGGGGCGATGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCt                                                                                                       <  6:42724/135‑1 (MQ=255)
                  aCGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGGGGCGGTGCGATGTGGGGCCGGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATAc                                                                                                  <  4:112651/135‑1 (MQ=255)
                         ccATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                           <  6:156090/135‑1 (MQ=255)
                         ccATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                           <  4:91710/135‑1 (MQ=255)
                                  cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                 <  7:241639/136‑1 (MQ=255)
                                   aTGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                 <  5:351906/135‑1 (MQ=255)
                                   aTGCTGGCGGTGGTGCTGACCCTGGGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                 <  6:417672/135‑1 (MQ=255)
                                       tGGCGTTGGTGCTGACCCTGGGGGGCGGTGCGAGGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACt                                                                            <  8:394978/136‑1 (MQ=255)
                                           gTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGCCCTGGTCGCGGTGGTGGTCTGGGGGGGGCGGATACCGCCCCTTGCAGTCCGGGGCCGGTCCt                                                                       >  7:267447/1‑136 (MQ=255)
                                               gTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCtgg                                                                     <  5:59045/135‑1 (MQ=255)
                                                             tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTTGTGATATGGGGTGGGCTGTTACCGCCACATACAATCCTGGCACGGGCCTGGTGGGTGGAACGccc                                                       >  3:115282/1‑135 (MQ=255)
                                                             tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                >  1:100956/1‑78 (MQ=255)
                                                             tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                <  2:100956/78‑1 (MQ=255)
                                                             tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTCTGGGGGGGGCTGATACCGCCCCTTCCCATCCTGGCACTGTCCTGGTGGCTGGAAGGccc                                                       >  3:77034/1‑135 (MQ=255)
                                                             tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATCGGGGGTGGGCTGATGCCGCCCCTTCCAGTCCTGGCACTGTCCTGGTGGCTGGAAGGccc                                                       >  6:186978/1‑135 (MQ=255)
                                                                 ggggCGAGGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCGCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGTCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                  <  8:375060/133‑1 (MQ=255)
                                                                 ggTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                  <  7:151121/136‑1 (MQ=255)
                                                                  gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGtt                                                                                                                         >  3:400369/1‑64 (MQ=255)
                                                                  gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGtt                                                                                                                         <  4:400369/64‑1 (MQ=255)
                                                                      gATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCCCCACGTCCATTGATGGCACAGTCCTGGTGGCTGGAAGGCCCCGCCCGCAtt                                             >  7:315960/1‑136 (MQ=255)
                                                                                cTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTGTTGGGTGGGGGGTTACCGCCCCATTCATTCCTGGCACTGTCCGGGTGGCTGGGAGGCCCCCAGCCCATTTGcccagcc                                    >  6:386394/1‑130 (MQ=255)
                                                                                cTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTCTGGGGGGGGGTGATTCCGCCCCTTCCCTTGCTGGCCCTGTGCAGGTGGGTGGAAGGCCCCGCGCGCCTTTGCCATGcc                                    >  6:303812/1‑135 (MQ=255)
                                                                                    gCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCtt                                  <  3:217888/133‑1 (MQ=255)
                                                                                                  cGACGGTTCGGCGCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCTCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGa                 <  7:86531/136‑1 (MQ=255)
                                                                                                   gACGGTTCGGCTCGGGCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGa                 <  1:215237/135‑1 (MQ=255)
                                                                                                      ggTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTGTGGGGTGGGCTGATACCGCCCCTTACATTCCTGGCACTGTCCTGGTGTGTGGAAGGCCCCCCGCGCAATTGCCATGCCTTGGCCAATATCAGGTGGAGttg             >  8:295484/1‑135 (MQ=255)
                                                                                                      ggTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATCTGGGGGGGGGTGATACCGCCACGTACAATACAGGCACTGTCCTGGTGGCGGGCAGGCCCCCACCGCAATAGCCATTCCTAGGGCAATAAAAGATGGAggt              >  2:111917/1‑133 (MQ=255)
                                                                                                                  tCGACGTGGTCGCGTTGGTGTGCTGGGGTGTGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGAGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc  <  1:111917/135‑1 (MQ=255)
                                                                                                                  tCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc  <  5:386394/135‑1 (MQ=255)
                                                                                                                  tCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc  <  4:77034/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                        
CACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCC  >  KT2440/3267682‑3267930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: