Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,323,335 G→T 5.0% F183L (TTC→TTA)  CJ019_03122 ← Carboxynorspermidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,323,3350GT 5.0% 255.0 / 2.7 80F183L (TTC→TTA) CJ019_03122Carboxynorspermidine synthase
Reads supporting (aligned to +/- strand):  ref base G (43/33);  new base T (2/2);  total (45/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

TGGCCGGTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCTGTTCA  >  KT2440/3323210‑3323462
                                                                                                                             |                                                                                                                               
tGGCCGGTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGAt                                                                                                                         <  2:182413/134‑1 (MQ=255)
   ccGGTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTcc                                                                                                                     >  2:446149/1‑135 (MQ=255)
   ccGGTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTcc                                                                                                                     >  5:384062/1‑135 (MQ=255)
     ggTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCggg                                                                                                                  >  8:164306/1‑136 (MQ=255)
     ggTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCgg                                                                                                                   >  6:215381/1‑135 (MQ=255)
                 gTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTtggtg                                                                                                      <  7:239113/136‑1 (MQ=255)
                  ttttGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTtggtg                                                                                                      <  6:316705/135‑1 (MQ=255)
                   tttGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTtggtgg                                                                                                     <  6:338011/135‑1 (MQ=255)
                       cGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGTAAGTTGATTTCCGGGTCGAAGTTGGTGGCaaa                                                                                                 >  5:380757/1‑135 (MQ=255)
                       cGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGTAAGTTGATTTCCGGGTCGAAGTTGGTGGCaaa                                                                                                 >  5:374027/1‑135 (MQ=255)
                        gAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGATGTTGGTGGCaaa                                                                                                 >  6:219346/1‑134 (MQ=255)
                        gAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCaaa                                                                                                 >  1:16233/1‑134 (MQ=255)
                        gAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCaaa                                                                                                 >  2:97926/1‑134 (MQ=255)
                        gAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAg                                                                                                >  7:283402/1‑135 (MQ=255)
                                     gCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCg                                                                                   <  7:164306/135‑1 (MQ=255)
                                      cAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCg                                                                                   <  6:384062/134‑1 (MQ=255)
                                        ggTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCCCTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTGGATTTCCGGGTCGCAGTTGGGGGCGAAGTACTTGCCATGCGAAc                                                                                >  5:283023/1‑135 (MQ=255)
                                        ggTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAAc                                                                                >  3:186220/1‑135 (MQ=255)
                                            gtcgGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCa                                                                           >  8:124668/1‑136 (MQ=255)
                                             tcgGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCAt                                                                          >  8:274032/1‑136 (MQ=255)
                                               ggTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGTAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCAtt                                                                         <  6:374027/135‑1 (MQ=255)
                                               ggTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTg                                                                        <  8:28980/136‑1 (MQ=255)
                                                gTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGTAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCAtt                                                                         <  6:380757/134‑1 (MQ=255)
                                                   cGCTTGACCTCGAACAGGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGt                                                                    <  8:227953/136‑1 (MQ=255)
                                                    gCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACg                                                                     <  4:17461/134‑1 (MQ=255)
                                                       tGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGt                                                                    >  3:417152/1‑132 (MQ=255)
                                                           cTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTTTACGGTGAACTCGCGGAAGTTGATTTCAGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGAGAACCGGCATTGACGTCGAGAat                                                             >  2:6524/1‑135 (MQ=255)
                                                           cTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGACAGCTCCACACTTGTCCGGTGAACTCGCTGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAat                                                             >  5:226650/1‑135 (MQ=255)
                                                              gAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAatat                                                           >  4:4136/1‑134 (MQ=255)
                                                               aaCATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGTAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATatc                                                          <  6:426047/134‑1 (MQ=255)
                                                               aaCATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATatc                                                          <  1:97926/134‑1 (MQ=255)
                                                                       gtTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGTCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATc                                                 >  7:130021/1‑135 (MQ=255)
                                                                       gtTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAAt                                                  >  6:408630/1‑134 (MQ=255)
                                                                        ttGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCaa                                               <  8:166287/136‑1 (MQ=255)
                                                                         tGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCaa                                               <  4:164374/135‑1 (MQ=255)
                                                                         tGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCaa                                               <  6:223357/135‑1 (MQ=255)
                                                                         tGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCaa                                               <  1:446149/135‑1 (MQ=255)
                                                                         tGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCaa                                               <  1:101272/135‑1 (MQ=255)
                                                                           cTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGTCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGc                                            <  8:130021/136‑1 (MQ=255)
                                                                            tGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGc                                            <  5:215381/135‑1 (MQ=255)
                                                                             ggTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGATGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATggg                                           >  3:311329/1‑133 (MQ=255)
                                                                              gTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGCGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTg                                          <  3:276763/135‑1 (MQ=255)
                                                                               tCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGt                                         <  5:341079/135‑1 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATatc                                                          <  1:372374/114‑1 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATatc                                                          <  1:377983/114‑1 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATatc                                                          >  2:372374/1‑114 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATatc                                                          >  2:377983/1‑114 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATaaa                                               >  4:193021/1‑125 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCaa                                               <  3:193021/125‑1 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGaa                                     >  4:431213/1‑135 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGaa                                     >  4:287549/1‑135 (MQ=255)
                                                                                   cTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGaa                                     >  1:85268/1‑135 (MQ=255)
                                                                                        tGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATAct                                >  1:446873/1‑135 (MQ=255)
                                                                                            cTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATActgctg                            >  1:152153/1‑135 (MQ=255)
                                                                                             tGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATActgctgc                           >  1:233421/1‑135 (MQ=255)
                                                                                                   cTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCa                    <  8:148927/136‑1 (MQ=255)
                                                                                                    tCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCa                    <  6:390268/135‑1 (MQ=255)
                                                                                                    tCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCa                    <  1:173513/135‑1 (MQ=255)
                                                                                                     ccACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGc                  >  7:292463/1‑136 (MQ=255)
                                                                                                       acacTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCtt                >  8:168144/1‑136 (MQ=255)
                                                                                                       acacTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCt                 <  6:396733/135‑1 (MQ=255)
                                                                                                         acTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATAGAATCAATGCTGTCGATATACTGCTGCTGCGCCCGCGTg               >  3:250073/1‑135 (MQ=255)
                                                                                                          cTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTgg              >  5:17751/1‑135 (MQ=255)
                                                                                                          cTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTgg              <  3:250086/135‑1 (MQ=255)
                                                                                                            tGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCg            >  3:319257/1‑135 (MQ=255)
                                                                                                               ccGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTa          <  2:233421/134‑1 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTTGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATActgctgct                          <  7:156383/117‑1 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATTCTGCTGCTGCGCCAGCTTGGCGTAGCt       <  7:274032/136‑1 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATActgctgct                          >  8:156383/1‑117 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCTCCAGCTTGGCGTAGc        >  1:47681/1‑135 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGc        >  5:284776/1‑135 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGc        >  5:123665/1‑135 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGc        >  6:328820/1‑135 (MQ=255)
                                                                                                                cGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCt       >  7:111651/1‑136 (MQ=255)
                                                                                                                cGGTGAACTAGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCt       >  7:7336/1‑136 (MQ=255)
                                                                                                                 ggTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCt       >  4:378860/1‑135 (MQ=255)
                                                                                                                 ggTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCt       <  1:250005/135‑1 (MQ=255)
                                                                                                                      aCTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCTGTTca  >  3:144950/1‑135 (MQ=255)
                                                                                                                        tCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCTGtt    <  4:258420/131‑1 (MQ=255)
                                                                                                                        tCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCTGtt    >  3:258420/1‑131 (MQ=255)
                                                                                                                             |                                                                                                                               
TGGCCGGTCAGGTACAGGTTTTGCGAGCCTACCACTGGCAGGTCGTCGGTACGCTTGACCTCGAACATGGTGTTGCTGGTCCACTGGCTGTTCTGCCAGCTCCACACTTGTCCGGTGAACTCGCGGAAGTTGATTTCCGGGTCGAAGTTGGTGGCAAAGTACTTGCCATGCGAACCGGCATTGACGTCGAGAATATCGATCGAATCAATGCTGTCGAAATACTGCTGCTGCGCCAGCTTGGCGTAGCTGTTCA  >  KT2440/3323210‑3323462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑