| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,432,242 | A→C | 25.9% | E36A (GAG→GCG) | gpFI → | Putative prophage major tail sheath protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,432,242 | 0 | A | C | 25.9% | 62.9 / 4.1 | 59 | E36A (GAG→GCG) | gpFI | Putative prophage major tail sheath protein |
| Reads supporting (aligned to +/- strand): ref base A (22/19); new base C (7/9); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCCGCGCCAAGGCGGTG > KT2440/3432108‑3432375 | cgcgTCCCTTGTCTTATCTGGAGCCCAATTGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGGCCTCCTCGATCATTGGCGTGGGCGACACCTTCACCGa < 1:337352/135‑1 (MQ=255)cgcgGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGa < 2:173590/135‑1 (MQ=255) ggCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACCCCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCGCGATCATTTGCTTGGTCGCCACCTTCCCCGCGGGc > 8:101079/1‑136 (MQ=255) cccTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCCATGCGCTGCCGTCCGCCGCGATCCTTGGCTTGGTCGCCACCTTCACCCCGGGCgg > 8:114696/1‑136 (MQ=255) ttGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACAACGTCACCGACGGCGGTAc > 8:365025/1‑136 (MQ=255) tCTGGAGCCCACATGAATGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACg < 8:104770/135‑1 (MQ=255) cTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCGACg < 1:347626/134‑1 (MQ=255) tGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACCCCGGGGCGCGCAGCATTGCGCTGCCGGCCTCCTCGCTCATTGGCTTGGTCGACCCCGTCACCCAGGGCGGGACCCCCCAGGcc > 7:61346/1‑136 (MQ=255) tGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGcc > 8:353700/1‑136 (MQ=255) tGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCAATTCGCGGCCGTCCACCGCGATCATTGGCTTGGTCCCCACCATCACCGACGGCGGTACACCCACGGc > 5:163580/1‑135 (MQ=255) gccccccACATGACTGGATTCTTTCACGGCGTTACCGAAACGAACGTCGCCACCGGGGCGCGCAGCATTGCGCTGCCGGCCGCCTCGCTCATTGGCTTGGGCGACACCTTCACCCAGGGCGGTACCGCCACGGcc < 4:47928/131‑1 (MQ=255) cccACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCCCGGCCAAGt > 4:96028/1‑135 (MQ=255) cccACATGACTGGATTCTTTAACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGAGCTGCCGTGCTCCTCGCTCATTGGGTTTGTCGACACCATAACCCCGGGCCGTAACGCCACCGCCAACTa > 8:12488/1‑136 (MQ=255) cccACATGACTGGATGCTTGCCCGGCGTTACCGTAACGACCGTCGACACCGGGGCGCGCAGCATTGCGCGGCCGTCCTCCTCGATCATTGGCTTGGGCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTa < 8:323697/136‑1 (MQ=255) cccaaTGACTGGATTCTTTCACGGCGTTACCGTAACCACCGTCGACACCGGGGCGCGAAGCTTGGCGCGGCCGTCCTCCGCGATCATTGGCTTGGGCGACACCTGCCCCGCGGGCGGTACCGCCACGGCCAAGTa < 6:85459/131‑1 (MQ=255) ccACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTTCGCTGGCGGCCTGCGCGATCATTGGGTTTGTGGACACCTTCACCGAGGGCGGGTCCCCCCCCGCCaacg > 6:353470/1‑132 (MQ=255) ccACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTTCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGCCACCTTCACCCCGGGCGGTTCCGCCACCGCCAAGt > 3:401556/1‑134 (MQ=255) ccACATGACTGGAGTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGCCCTCCTCGATCATGGGCTGGGGCGACACCTGCCCCGAGGGCGGTACCGCCACGGCCAAGTa < 4:227853/135‑1 (MQ=255) ccAAATGAGTGGATTCTTACAGGGCGTTCCCTAAACGACCGTCGACACCGGGGCGCGCAGATTTGCGCGGCCGTCCGCCTCGATCATTGGCTGGGTCGACACCTTCACCGCGGGCGGAACCCCCACGGCCAAGTa < 4:201763/135‑1 (MQ=255) caTGCCTGGATTCTTTCACGGCGTTACCGAAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACaa < 4:85049/135‑1 (MQ=255) aTGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACg < 7:365025/136‑1 (MQ=255) aCTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCCGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACCCCTTCCCCGCAGGCGGTACCGCCACCGCCAACGACAACGccc > 8:84759/1‑136 (MQ=255) gATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGCGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGt < 7:353700/136‑1 (MQ=255) gATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTa < 7:230394/134‑1 (MQ=255) aTTCTTCCCCGGCGTCCCCGTACCCACGGTCGACCCCGGGGCGCGCAGCATTGCGCGGCCGTCCGCCTCGATCTTTGGCTTGGTCGACACCTTCCCCGCGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGt < 5:266492/135‑1 (MQ=255) tCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGc < 2:140658/135‑1 (MQ=255) tCTGTCACGGCGTTACCTTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGc < 8:439918/135‑1 (MQ=255) cTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCCTTGCGCTGGCCTGCACCGCGCTAATTGGGGTGGTCGCCCCCTTCACCCCGGGCGGTACCCCCCCGGCCCAGTACAACGCCCAAGTggt > 2:142173/1‑133 (MQ=255) aacgTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTGCTCCTCGATAATTGGCTTGGTCGCCACCTTCACCGAGGGCGGTACCCCCACGGCCAAGTACAACGACCTAGTGCTGATCACAAACGAGCGCGAGGCg > 5:296725/1‑135 (MQ=255) aacgTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGCTCATTGGCGTGGTCGACACCTTCACCGAGGGCCGGCCCGCCACGGCCAAGTACAACCACCTAGTGCGGATCACCAACGAGCGCGAGGCg > 1:257274/1‑135 (MQ=255) aacgTCGACACCGGGGCGCGCAGCAATGCGCTGCCGTCCTACGCGATCAATGGCTTGGGCGCCACCCTCACCGCGGGCGGTAACGCCACGGCCAACTACAACGACCTAGTGCTGATCAACAACGAGGGCCAGGCg > 4:313053/1‑135 (MQ=255) cGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGGCGACACCTTACCCGAGGGCGGTACCGCCCCGGCCAAGTACAAAGACCTAGTGCTGATCACCAACGCGCGCGCGGCGGTcgcc > 7:51230/1‑136 (MQ=255) gcgcgcAGCTTTGCGCGGCCGTCCTCCTCGATCATGGGCTTGGTCCACACCTTCCCCGCGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGt < 2:341046/135‑1 (MQ=255) cgcgcAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCCCCGAGGGCGGTTCCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGt > 3:410598/1‑134 (MQ=255) cgccgcATTGCGCGGCCGTCCTCCTCGATCATGGGCGGGGCCCACCCCTTCCCCGCGGGCGGTACCCCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGaaa < 7:77036/136‑1 (MQ=255) cgtgcctccgCGATCATTGGCTTGGCCGACACCTTCACCGAGGGCGTTACCCCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCAcc < 5:111373/131‑1 (MQ=255) ccGGCCGCCGCGATCATTGGCGTGGGCGACACCTTCCCCGTGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCAcc < 6:247309/135‑1 (MQ=255) cGTCCTCCTCGATCATGGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCAcc < 2:332866/134‑1 (MQ=255) tcctccTCGATCATTGGCTTGGTCGACACCTTCACCGa < 4:188849/38‑1 (MQ=255) tcctccTCGATCATTGGCTTGGTCGACACCTTCACCGa > 3:188849/1‑38 (MQ=255) tcctccTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAgg > 7:29079/1‑136 (MQ=255) tcctccTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGGGAAACCTCGGCGATCACCAAgg > 7:187046/1‑136 (MQ=255) ctcctcGATCATGGGCTTGGGCGACACCTTCACCGCGGGCGGTCCCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGc < 1:38389/135‑1 (MQ=255) ccgcGATCATTGGCTGGGCCCCCACCTCCCCCGCGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGt < 5:442067/132‑1 (MQ=255) cgcGATCTTTGGCTTGGTCGACCCCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGc < 7:233717/134‑1 (MQ=255) tcGATCATTGGCTTGGTCGACACCTTCACCGCGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCa < 8:337572/136‑1 (MQ=255) gctCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCa < 7:362510/132‑1 (MQ=255) agcATTGGCTGGGGCGACACCTGCACCGAGGGCGGCACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCa < 6:161738/131‑1 (MQ=255) aTCCTTGGCTGGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCa < 4:94713/133‑1 (MQ=255) aTCATTGGCTGGGACCACACATCCCCCGGGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCa < 5:353470/133‑1 (MQ=255) ttGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCa > 4:318227/1‑135 (MQ=255) cTTGGTCGACACCTTCACCGAGGCCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCt > 4:375146/1‑134 (MQ=255) ttGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTaca > 7:119106/1‑136 (MQ=255) cGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGCTCACCCAGGCGTGCAAGGCCATCAACACCcgc > 7:383613/1‑136 (MQ=255) cGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGc < 4:331974/114‑1 (MQ=255) cGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGc > 3:331974/1‑114 (MQ=255) acacCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGCCCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCcgcg > 4:338272/1‑135 (MQ=255) acacCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTacac < 2:350546/130‑1 (MQ=255) acacCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTacac > 1:350546/1‑130 (MQ=255) cTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCCGCGCCaa > 3:391130/1‑135 (MQ=255) ccccGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCCGCGCCAAGGc < 8:354117/133‑1 (MQ=255) cGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCCGCGCCAATGCgg > 6:253878/1‑134 (MQ=255) cGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCCGCGCCAAGGCGGTg > 8:322183/1‑136 (MQ=255) | CGCGGCCCTTGTCTTATCTGGAGCCCACATGACTGGATTCTTTCACGGCGTTACCGTAACGAACGTCGACACCGGGGCGCGCAGCATTGCGCTGCCGTCCTCCTCGATCATTGGCTTGGTCGACACCTTCACCGAGGGCGGTACCGCCACGGCCAAGTACAACGACCTAGTGCTGATCACCAACGAGCGCGAGGCGGTCGCCGCGTTCGGTGAAACCTCGGCGATCACCAAGGCGTGCAAGGCCATCTACACCCGCGCCAAGGCGGTG > KT2440/3432108‑3432375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |