| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,440,343 | A→C | 100% | S18R (AGC→CGC) | CJ019_03266 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,343 | 0 | A | C | 80.9% | 12.1 / ‑1.1 | 26 | S18R (AGC→CGC) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base C (17/3); total (18/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG > KT2440/3440211‑3440468 | gATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGccgcccgc > 5:128638/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCACCCCGGCCGGCGCCCGGGc > 2:121317/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGc > 5:33406/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAACCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCCCCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCCTGCGGCGCCCGGGc > 6:211104/1‑135 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCCCGGGCGGCGCCCGCGCCGCCTgcgg > 5:449409/1‑132 (MQ=255) aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCGGGCGGCGCCCCGGCCGCCGGGGGGTggccggccca > 3:13964/1‑133 (MQ=255) gaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGGGGCCAACg > 5:366223/1‑135 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCACCGccga > 6:219023/1‑132 (MQ=255) tACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCTGCGGGTGGCCGGCCACCGCCTAAAAGGCGCAGcg > 7:1115/1‑135 (MQ=255) gagcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCACGCCCCCGCCGATAATGCGCCgcgcgcggg > 3:258480/1‑132 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGTGTGGCACGCCAACCCCAATAAAGCGCCGCGCGCGGa > 6:220371/1‑134 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCAGGCCCACCCCAATAAGGCGCCGCTCGCTgc > 4:373420/1‑133 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCCACCCCCATAAGGCGCAGCCCGCGGaggag > 3:113890/1‑131 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGTGGGGGCGGCCACCGCCGAGAAGGCGCAGCGCGCGGGTCAg > 2:3975/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTGGCGGCGCCCCGGCCGCCGGGGTGTGGGAGGCCCACGCCGATAAGGCCCCGCGCGCGGAGCCGGc > 4:319434/1‑135 (MQ=255) gcgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGGCCGGGCCGCCGGGGTGGGGCCGGCCCACCCCGATAAGGCGCCGCGCGCGGAGCAGGCCGCGGCCTTCGGcca > 3:144455/1‑131 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCACCCCCGATAATGCGCCGCGCGCGGCTCCGGGCGCTGCCGTCGGGACGCAg > 2:292698/1‑135 (MQ=255) ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCCCGCCGATAAGGCGCAGCTCGCTGAGCAGGCCGAGGACTTCGGCCAGCAGtt > 4:168465/1‑135 (MQ=255) gcGCCGGGGCGCTGCTGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:73747/135‑1 (MQ=255) ggCCGGGGCGCGGGGGCGGGCCGCGTGCGCGCGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 5:211104/134‑1 (MQ=255) cgggggggCGCGGCGGCGGGCCGCCGGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 6:128638/129‑1 (MQ=255) cgcgggggCGCTGGGGCGGGGCGCCCGCGCGGGCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 5:220371/129‑1 (MQ=255) cgcgggggCGCTGCTGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 3:224472/129‑1 (MQ=255) cGCCGGGGCGGGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCACGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 4:406673/135‑1 (MQ=255) tGGCGCTGCTGCTGGCCGCCCGCCCAGGGGGCGGCCCGGCCGCCGGGGGGTGGGAGGCCCCCGCCGATAATGCGGACCGCGCGGCTCAGGGGGCGTACTTAGGGAAGCACTTGGGGGGGGAGGTTCGCGCGCCCg > 4:116564/1‑135 (MQ=255) ctgctgctgGCCGCGAGCGCTGACGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg < 2:9498/135‑1 (MQ=255) gcggCCGCGCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 1:121317/133‑1 (MQ=255) | GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG > KT2440/3440211‑3440468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |