Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,496,362	A→G	5.8%	intergenic (‑290/+704)	CJ019_03314 ← / ← CJ019_03315	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,496,362	0	A	G	5.8%	225.8 / 3.4	71	intergenic (‑290/+704)	CJ019_03314/CJ019_03315	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (41/25);  new base G (2/2);  total (44/27)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CACCATCAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGATTATTGGATAATCAAG  >  KT2440/3496231‑3496497                                                                                                                                    |                                                                                                                                        caccaTCAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAgcg                                                                                                                                      >  3:2255/1‑135 (MQ=255)      tCAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGATGTTCTCGCAAGCGCTTCgg                                                                                                                                >  8:403199/1‑136 (MQ=255)      tCAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCg                                                                                                                                 >  6:74300/1‑135 (MQ=255)      acAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTCCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCg                                                                                                                                 >  6:114086/2‑135 (MQ=255)       cAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCgg                                                                                                                                >  6:193043/1‑135 (MQ=255)       cAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCgg                                                                                                                                >  5:38869/1‑135 (MQ=255)       cAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGc                                                                                                                               >  7:110388/1‑136 (MQ=255)          ggCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCg                                                                                                                            <  8:325382/136‑1 (MQ=255)          ggCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAACTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTc                                                                                                                             <  2:139360/135‑1 (MQ=255)               tCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCt                                                                                                                        <  7:257442/135‑1 (MQ=255)                 gagaTACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTgg                                                                                                                      >  4:118792/1‑135 (MQ=255)                 gagaTACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTgg                                                                                                                      >  6:447901/1‑135 (MQ=255)                 gagaTACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTgg                                                                                                                      >  2:348675/1‑135 (MQ=255)                 gagaTACTCATTAAACTGTTTGTTGCTAAGTCCTCGGGTGCAAAAGACTCTCCATGTTTTAGTTGTTGATCGTGAGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCTACCTGGc                                                                                                                     >  7:251204/1‑136 (MQ=255)                  agaTACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTgg                                                                                                                      <  4:291341/134‑1 (MQ=255)                      aCTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGAt                                                                                                                 <  2:78089/135‑1 (MQ=255)                       cTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATg                                                                                                                >  2:216967/1‑135 (MQ=255)                       cTCATTAATCTGTTTGTTGATAAGGCCTCGGGTGCAAAAGTCTCTGCATGTGTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCGTCGCATCGCCCTGGCTCATg                                                                                                                >  5:69365/1‑135 (MQ=255)                         cATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATg                                                                                                                >  2:101513/1‑133 (MQ=255)                           ttAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGgag                                                                                                            <  5:50411/135‑1 (MQ=255)                           ttAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGgag                                                                                                            <  6:178773/135‑1 (MQ=255)                               tCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATgg                                                                                                        >  4:204543/1‑135 (MQ=255)                                  gtttgttGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTcc                                                                                                     >  1:240803/1‑135 (MQ=255)                                    ttgttgCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTc                                                                                                      <  3:285616/132‑1 (MQ=255)                                    ttgttgCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTc                                                                                                      <  6:428704/132‑1 (MQ=255)                                      gttgCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCtt                                                                                                   >  6:360109/1‑133 (MQ=255)                                       ttgCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCGTGTTTCGCATGAAGTTCTCGCATGCGCTTCGGCTCTCCCTGGCTGATGGGAGATGGTCCTGTaa                                                                                                >  2:327215/1‑135 (MQ=255)                                       ttgCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAg                                                                                               >  7:55347/1‑136 (MQ=255)                                                ccTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAGGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTc                                                                                        >  2:76215/1‑134 (MQ=255)                                                ccTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTcc                                                                                       >  8:102070/1‑135 (MQ=255)                                                ccTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCg                                                                                      >  7:87102/1‑136 (MQ=255)                                                  tCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGa                                                                                     <  4:398312/135‑1 (MQ=255)                                                  tCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGa                                                                                     <  4:427088/135‑1 (MQ=255)                                                     ggTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGaa                                                                                    >  6:197184/1‑133 (MQ=255)                                                                    gCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCg                                                                  >  8:159274/1‑136 (MQ=255)                                                                    gCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCg                                                                  >  7:216636/1‑136 (MQ=255)                                                                      aTGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCg                                                                  >  6:396580/1‑134 (MQ=255)                                                                          ttttGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCTGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAAt                                                             <  2:374413/135‑1 (MQ=255)                                                                          tttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAGGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAAt                                                             <  1:76215/135‑1 (MQ=255)                                                                          tttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAAt                                                             <  2:43435/135‑1 (MQ=255)                                                                          tttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAAt                                                             <  4:2255/135‑1 (MQ=255)                                                                           ttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAGgc                                                                                                                                       >  7:289308/1‑60 (MQ=255)                                                                           ttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAGgc                                                                                                                                       <  8:289308/60‑1 (MQ=255)                                                                           ttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTc                                                                                                      >  3:374514/1‑93 (MQ=255)                                                                           ttAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTc                                                                                                      <  4:374514/93‑1 (MQ=255)                                                                            tAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATgg                                                           >  3:159980/1‑135 (MQ=255)                                                                                tgttgATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGAATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGggcgg                                                       >  1:275462/1‑135 (MQ=255)                                                                                     aTCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTgg                                                 >  7:151426/1‑136 (MQ=255)                                                                                       cGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCt                                                                           >  1:340790/1‑108 (MQ=255)                                                                                       cGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCt                                                                           <  2:340790/108‑1 (MQ=255)                                                                                       cGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAAt                                                             <  6:436934/122‑1 (MQ=255)                                                                                       cGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAAt                                                             >  5:436934/1‑122 (MQ=255)                                                                                       cGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGa                                               >  7:154299/1‑136 (MQ=255)                                                                                            gcTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGc                                            >  2:418588/1‑134 (MQ=255)                                                                                              tACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGAt                                                                                                                 >  8:88647/1‑63 (MQ=255)                                                                                              tACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGAt                                                                                                                 <  7:88647/63‑1 (MQ=255)                                                                                              tACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGTATGCCg                                          >  3:255343/1‑134 (MQ=255)                                                                                                           tCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGAtttt                            >  7:395236/1‑135 (MQ=255)                                                                                                           tCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGAttt                             >  4:304672/1‑134 (MQ=255)                                                                                                           tCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTCGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGAtttt                            >  8:420184/1‑135 (MQ=255)                                                                                                              tGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAg                         <  3:118792/135‑1 (MQ=255)                                                                                                                     cATGAAGTTCTCGCTAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGa                  <  6:185480/135‑1 (MQ=255)                                                                                                                     cATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGa                  <  1:418588/135‑1 (MQ=255)                                                                                                                     cATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGa                  <  5:447901/135‑1 (MQ=255)                                                                                                                      aTGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGa                  >  3:70909/1‑134 (MQ=255)                                                                                                                        gAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGatta               >  3:76204/1‑135 (MQ=255)                                                                                                                        gAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGatta               <  4:159980/135‑1 (MQ=255)                                                                                                                        gAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGatta               >  2:183029/1‑135 (MQ=255)                                                                                                                          aGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGattat              <  5:360109/134‑1 (MQ=255)                                                                                                                              ctcGCAAGCGCTTCGGCTCGTCCTGGATGATTGGAGATGGTCGTTTAAGGAAACTGCGTAGAAGTTGCTGATCAGTAGGATTTGGGCGGATTTGTTATGCCGAAAAGCCCGATTTTCAGTCGCTGattattttaa         <  8:251204/135‑5 (MQ=255)                                                                                                                                 gCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGATAAGCCCGATTTTCAGTCGCTGATTATTGGATaa       >  4:24674/1‑134 (MQ=255)                                                                                                                                    aGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCGTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGATTATTGGATAATCAAg  <  8:154299/136‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CACCATCAGGGCTGTCGAGATACTCATTAATCTGTTTGTTGCTAAGGCCTCGGGTGCAAAAGTCTCTGCATGTTTTAGTTGTTGATCGTGCGCTACAAAGTCAGGGTCCTGTTTCGCATGAAGTTCTCGCAAGCGCTTCGGCTCGACCTGGCTGATGGGAGATGGTCCTTTAAGGAAACTCCGAACAAGTTGCTCATCAGTCGGAAATGGGCGGCTTTGGGATGCCGAAAAGCCCGATTTTCAGTCGCTGATTATTGGATAATCAAG  >  KT2440/3496231‑3496497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑