| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,592,468 | C→A | 5.6% | W98C (TGG→TGT) | hcaD ← | 3‑phenylpropionate/cinnamic acid dioxygenase ferredoxin‑‑NAD( ) reductase component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,592,468 | 0 | C | A | 5.6% | 219.8 / 2.9 | 72 | W98C (TGG→TGT) | hcaD | 3‑phenylpropionate/cinnamic acid dioxygenase ferredoxin‑‑NAD( ) reductase component |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base A (2/2); total (33/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
CCCAGCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAACCGCCGCAGACTA > KT2440/3592339‑3592601 | cccAGCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCCCTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGAc > 6:213948/1‑135 (MQ=255)cccAGCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGAc > 4:325893/1‑135 (MQ=255) ccgCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACtt < 1:40232/133‑1 (MQ=255) cAGCGACGCCATGCGCCAGCAGGGGGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACtt < 1:314929/135‑1 (MQ=255) cAGCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACtt < 3:206324/135‑1 (MQ=255) aGCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGGTCGGTTGCCGGCAGGGGCTGGTCCTCGACCCAGCCTTc > 6:118112/1‑135 (MQ=255) aaGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGTGCGTTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGc < 8:121521/136‑1 (MQ=255) aGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGc < 4:251919/135‑1 (MQ=255) tGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGCCCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTg > 3:346839/1‑135 (MQ=255) gcgcgagctgGGTGTCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGAt < 7:308313/131‑1 (MQ=255) gAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGa < 4:239327/135‑1 (MQ=255) gAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGAt > 7:220615/1‑136 (MQ=255) cgCCCGCTGGGGGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGAt < 5:248976/134‑1 (MQ=255) gCCAGCGGGGTGGCGACGGCGGCGATGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCGAGACTTCATCGCCCTTGAt < 1:279268/134‑1 (MQ=255) gCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGAt < 3:50067/134‑1 (MQ=255) gCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGAt < 6:113641/134‑1 (MQ=255) gggTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGCCCCAGCCGTCATCGCCCTTGATCCACGccg > 4:60913/1‑134 (MQ=255) gggTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGccat > 7:400555/1‑134 (MQ=255) cGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCCGACTTCATCGCCCTTGATCCACGCCTTGTAgcg > 7:321431/1‑136 (MQ=255) cGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAgc > 6:147856/1‑135 (MQ=255) gcggcgCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAgcggcg > 8:135251/1‑134 (MQ=255) gcgcTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGGTGCCGGCAGGGGCTGGTCCTCGCCCCAGACGTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAgg > 6:165873/1‑135 (MQ=255) cACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAg < 1:327676/135‑1 (MQ=255) aCCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAggc > 8:9678/1‑136 (MQ=255) ggCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACACAGACTTCATCGCCCTTGCTCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAggcgg > 2:351598/1‑135 (MQ=255) ggCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACACAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAggcgg > 2:345594/1‑135 (MQ=255) gcCGACCACTACGAAGCAGCGGGCATCGCTGTGACTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTc < 1:125616/135‑1 (MQ=255) ccGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTca > 4:235608/1‑135 (MQ=255) cGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCTACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTcc > 6:423457/1‑133 (MQ=255) cGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTcac < 3:170768/135‑1 (MQ=255) cGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTcac < 1:267086/135‑1 (MQ=255) agcGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAggcggc < 3:320316/116‑1 (MQ=255) agcGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAggcggc > 4:320316/1‑116 (MQ=255) agcGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACcgtcgt > 8:313374/1‑135 (MQ=255) ggCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAAc < 2:271700/135‑1 (MQ=255) ggCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAAc < 2:148595/135‑1 (MQ=255) gCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACgg > 8:170944/1‑136 (MQ=255) gCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACg > 3:109409/1‑135 (MQ=255) gCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACg > 4:180948/1‑135 (MQ=255) gCATAGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGCGCTGGTCTTCGACCCAGACTTCATCGCGCGGGATCCACGGCGTGTAGCGGCGCAGGTCGTCGCGTGCAGGCGGCTCACAACCCCCACCGTCGTAAAccc > 8:434239/1‑134 (MQ=255) cATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGc < 8:220615/136‑1 (MQ=255) cGCTGTGCCTGGGCGGTTCGGGTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGa < 3:383252/135‑1 (MQ=255) cTGTGCCGGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGa < 5:277331/133‑1 (MQ=255) gtgCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAgcg < 1:288145/78‑1 (MQ=255) gtgCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAgcg > 2:288145/1‑78 (MQ=255) tgCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAg < 7:170944/136‑1 (MQ=255) cTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCACAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCt > 3:336127/1‑135 (MQ=255) tGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACCAACCCCACCGTCGTCAACGGCGAACGTAGCtt > 1:183998/1‑135 (MQ=255) tGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGCACGTAGCtt > 1:403879/1‑135 (MQ=255) gggCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGCACGTAGCttt > 3:389780/1‑135 (MQ=255) cggttcggttGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTtgtg < 5:301620/135‑1 (MQ=255) cggttGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAgg > 7:388870/1‑136 (MQ=255) cggttGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAgg < 7:358767/136‑1 (MQ=255) cggttGCCGGCAGGGGCTGGTCAGCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAgg < 7:266004/136‑1 (MQ=255) ggttGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAAAGGCGAACGTAGCTTTGTGCCAAgg > 2:277038/1‑135 (MQ=255) gCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCa < 3:110107/135‑1 (MQ=255) ggggCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCa < 2:36062/135‑1 (MQ=255) cTGGTCATCGACCCAGACTTCATCGCCCTTGCTCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGCCCGTCGCGTTGTGGCAAGGGCCGACCAGCAACccg > 7:165319/1‑136 (MQ=255) tGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 6:394581/135‑1 (MQ=255) tGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 4:194165/135‑1 (MQ=255) tGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 4:346839/135‑1 (MQ=255) tGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGGCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 3:311279/135‑1 (MQ=255) tGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGGCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 4:136239/135‑1 (MQ=255) tGGTCATCGACACAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 1:351598/135‑1 (MQ=255) tGGTCATCGACACAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccg < 1:345594/135‑1 (MQ=255) gggcATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccgcc < 8:116693/133‑1 (MQ=255) gTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAAccgcc < 7:251761/135‑1 (MQ=255) aTCGACTCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAATCCAGCAAACCGCCGCa > 5:257920/1‑135 (MQ=255) aTCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGACCGTAGCTTTGTGCCAAGGGCAAACCAGCAAACCGCCGCa > 2:394850/1‑135 (MQ=255) aTCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGCCGGCTCACAACCCACCCCGTCGTCAACGGCGCACGTAGCTTTGTGCCAAGGGCAACCCCGCAAAccgccg > 7:272987/1‑133 (MQ=255) tCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAACCGCCGCAGa < 7:317866/136‑1 (MQ=255) cGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGGCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAACCGCCGCAGa < 3:56730/135‑1 (MQ=255) cccAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTc < 4:107429/46‑1 (MQ=255) cccAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTc > 3:107429/1‑46 (MQ=255) cccAGACTTCATCGCCCTTCATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCCTACTCAACCGCGAACGTAGCTTAGTGCCAAGCGCAAACCAGCAAACCGCAGCAGACTa > 3:189951/1‑135 (MQ=255) ccAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGa > 4:286391/1‑89 (MQ=255) ccAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGa < 3:286391/89‑1 (MQ=255) | CCCAGCGAAGCCATGAGCCAGCAGGGTGGCGACGGCGGCGCTGCCGGCGGCACCGGCGCCGACCACTACGAAGCAGCGGGCATCGCTGTGCCTGGGCGGTTCGGTTGCCGGCAGGGGCTGGTCATCGACCCAGACTTCATCGCCCTTGATCCACGCCTTGTAGCGGCGCAGGTCGGCGAGTGCAGGCGGCTCACAAACCACACCGTCGTCAACGGCGAACGTAGCTTTGTGCCAAGGGCAAACCAGCAAACCGCCGCAGACTA > KT2440/3592339‑3592601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |