Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,793,904 A→C 30.8% intergenic (+57/+22) CJ019_03601 → / ← argD_3 hypothetical protein/Acetylornithine/succinyldiaminopimelateaminotrans ferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,793,9040AC30.8% 49.3 / 3.0 41intergenic (+57/+22)CJ019_03601/argD_3hypothetical protein/Acetylornithine/succinyldiaminopimelateaminotrans ferase
Reads supporting (aligned to +/- strand):  ref base A (13/14);  new base C (10/4);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTC  >  KT2440/3793782‑3794039
                                                                                                                          |                                                                                                                                       
gCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGTCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCcgcg                                                                                                                            >  7:319875/1‑136 (MQ=255)
  aTTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCGCGCCAGATAGGTGCACCCCCCGGCGGGCCCCAGCGCCCGCCCGGCCCccgc                                                                                                                           >  5:82481/1‑135 (MQ=255)
       cAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGGCAGATAGGTGCACCGCAGGGCGTGCCCCTGCGCCCGCCCTGCCCCCGCTGAAAc                                                                                                                     >  8:105927/1‑136 (MQ=255)
       cAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCCGGGCGTGCACAAGCGCACGCCCCGCCCGCGCGGaaa                                                                                                                      >  6:449677/1‑135 (MQ=255)
            gCGGCCGGCCGGCATGGCCAGGCGCGCTACGGCCTGCGCATCCGGGGCGCTTGATGCTGCCAACCTGCCGGGCGCGCCATATAGGTGCACCGCAGGGCGGGCACAGGCGCCCGCCCGGCCCGCGCTGAAACGCTa                                                                                                                 <  2:292103/135‑1 (MQ=255)
                  ggCCTGCATGGCCAGGCTCGCGCAGGCCTGAGCCTCAGGGGCCCTTGATGCTGGCAAACACGACGGCTCGCCAGATAGGTGCCCCCCCGGGCGGGGCCATCAGCACCCCCTGGCCGCCCTGAAACCCCAATTCat                                                                                                           >  4:278378/1‑134 (MQ=255)
                  ggCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCCACCTGCCCGGCTCGCCAGATAGGTGCACCGCACGGCGTGCCCATGCGCACGCCCTGCCCGCGCGGAAACGCGATTTCAgc                                                                                                          >  7:362458/1‑136 (MQ=255)
                  ggCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCCACCAGCCCGGCTCGCCAGATTGGTGCACCCCCCGGCGGGGACAAGCGCCCGCCCAGCCCCCGCGGGAACCCGAtttgcg                                                                                                           >  2:102164/1‑132 (MQ=255)
                         aTGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCCCCGCCGGGCGTGCCCATGCGCCCGCCCTGCCCGCGCGGCAACGCTATTTCCGCGCCGCgg                                                                                                   >  7:416386/1‑136 (MQ=255)
                            gCCAGGCTCGCGAAGGCGTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGGGCACCGCAGGGCGTGCACATGCCCACGCCGTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGa                                                                                                <  7:293178/136‑1 (MQ=255)
                             cctggCGCGCGACGGCCTGAGCATCTGGGGCGGTTGTTGCTGGAAACCTGCCCGGCGCGCCAGATCGGGGCACCGCCGGGGGTGCACATGCGCACGCCGGGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGa                                                                                                <  3:389064/132‑1 (MQ=255)
                             ccAGGCTGGCGATGGCCTGCGAATCAGGGGCGCTTGAGGCGGGCACCGGGCCGGGCGCGCCTTATAGGGGCCCGGCCGGGCGTGAACATGCGCCCGCCCGGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGa                                                                                                <  4:13314/135‑1 (MQ=255)
                             ccAGGCGCGCTAAGGCGTGAGCATCTGGGGCGCTTGATGCTGGCAACCTGCCGGGCTCGCATGATAGGTGCACCGCTGGGCGTGCACATGCCCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGa                                                                                                <  1:346767/135‑1 (MQ=255)
                                   tCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCCCGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGt                                                                                          >  6:150808/1‑135 (MQ=255)
                                       gAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGTGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCttt                                                                                      <  4:132699/135‑1 (MQ=255)
                                         aGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCCCGCCCTGCCCGCGCTGCAACGCGATTTCAGCGCCGCGGTGAAGCGGTCTTTCa                                                                                    >  1:299346/1‑135 (MQ=255)
                                          ggCCTGAGCAACAGGGGCTCTTGAGGCAGCCAACCTCCCCCGAGCCCCAAATAGGGCCACCGCAGGGCTTGCACATGCCCACCCCGTGCCCGCGCTGAAACCCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAg                                                                                   <  6:366087/135‑1 (MQ=255)
                                              tGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCCGGGCGTGGGCAAGCGCCCGCCCGGCCCCCGCGGCAACCGGAATTAAGCGCCCCGGGGACGCGGTCGTTAAGcccc                                                                               >  5:67755/1‑135 (MQ=255)
                                              tGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCCGGGCGTGCACATGCGCACGCCCTGCCCCCCCGGAAACGCTATTTCAGCGCCGCGGGGAAGCGGGCGTTCCGCAcc                                                                               >  4:270968/1‑135 (MQ=255)
                                                   aTCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCaag                                                                          <  2:325080/135‑1 (MQ=255)
                                                       ggggggCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAgaagga                                                                     <  7:239559/136‑1 (MQ=255)
                                                         ggCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAACCGCTATTTCATCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAgaaggaag                                                                   >  7:331315/1‑136 (MQ=255)
                                                              ttGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCCGGGCGTGCCCATGCGCCCGCCCCGCCCGCCCTGAAACCCTATTTCAGCGCCGCGGTGACGCTGGCGTTAACCACCTCAAGAACGAACTCCg                                                               >  6:28710/1‑135 (MQ=255)
                                                                gATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCCCCTCAAGAAGGAAGTCCGCa                                                             >  3:77363/1‑135 (MQ=255)
                                                                    cgggCAACCTGCCCGGCCCGCACGATAGGTCCCCCGCCGGGCGCGCACTGGCGCAGGCCCGGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTg                                                          <  1:138300/132‑1 (MQ=255)
                                                                     tGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACCTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGGGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGc                                                        >  4:155715/1‑135 (MQ=255)
                                                                     gggaaaCCGGCCCGGCTCCCCTGATCGGGCCACCGCAGGGCGTGCACATGCGCCCGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTg                                                          <  3:25141/129‑1 (MQ=255)
                                                                          aCCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCCCGCCCTGCCCGCGCTGCAACGCGATTTCATCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAACAAGGAAGTCCGCAATGTCCCGGCt                                                  >  8:160311/1‑136 (MQ=255)
                                                                                 cGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGaaaa                                             >  3:163183/1‑134 (MQ=255)
                                                                                  ggCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAatca                                          >  8:287436/1‑136 (MQ=255)
                                                                                     gcGCCAAATAGGGCCCCCCCCGGGGGGGCACATGCCCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCg                                       <  7:264731/135‑1 (MQ=255)
                                                                                       gCCAGATAGGTGCACCGCAGGGCGTGCACATTCGCACGCCCGGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGaa                                     <  8:319875/136‑1 (MQ=255)
                                                                                            attggTGCAGCGCAGGGCGGGCACTGGCGCCCGCGGGGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGcc                                 <  1:102164/132‑1 (MQ=255)
                                                                                                gTGCACGGCAGGGCGGCCACTTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGGGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCAt                             <  2:11730/135‑1 (MQ=255)
                                                                                                 tGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCAt                             <  2:11727/134‑1 (MQ=255)
                                                                                                      cGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCAttttga                        >  1:68277/1‑132 (MQ=255)
                                                                                                      cGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAg                       >  1:366750/1‑135 (MQ=255)
                                                                                                      cGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATAATCGAAGGCCGCATTTTcc                        >  6:418690/1‑133 (MQ=255)
                                                                                                         aGGGCGTGCACAGGCGCACGCCCGGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCACGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCAc                    <  3:187015/135‑1 (MQ=255)
                                                                                                           ggCGTGGACCTGCGCCCGCCCTGCCCCCGCGGCAACGGGATTTCCCCGCCGCGGTGAAGCTGGCTTTCAGCACCTCAAGAAGGAAGGCCGCCTTGGACCGGGTGCAAATCATAGACGGCCGCCTTTTCCGCagggg                 >  8:168384/1‑132 (MQ=255)
                                                                                                                      gcgcACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGGGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGc      <  7:16017/136‑1 (MQ=255)
                                                                                                                          aCGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCtt   >  5:20075/1‑135 (MQ=255)
                                                                                                                          aCGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCAGTTTCAGCACGTTGTCATGCGGGCCTTc  >  8:97293/1‑136 (MQ=255)
                                                                                                                          |                                                                                                                                       
GCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTC  >  KT2440/3793782‑3794039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑