Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,891,748	G→C	8.2%	L346V (CTG→GTG)	CJ019_03683 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,891,748	0	G	C	8.2%	171.6 / 6.2	58	L346V (CTG→GTG)	CJ019_03683	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/23);  new base C (3/2);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCTGGCAGTTACTCAACTACTCGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACTTTGCCGTAC  >  KT2440/3891615‑3891882                                                                                                                                      |                                                                                                                                       tCCCTGGCAGTTACTCAACTACTCGTGACTCTTTCGATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCACCGCTGCCTCCGTAGAGATAAATCTTGTGCAgg                                                                                                                                       <  5:149463/135‑1 (MQ=255)        cAGTTACTCAACTACTCGTGACTCTTTCGATGGTTGGCGGTGACTGCAAGCTGGCGTAACGTCTGCGGAATGTTTACACATACGGTTCGCCTGCCGCGCTGCCACCGTAGAGATAAATCTTGTGCAGGccacga                                                                                                                                 <  5:332438/134‑3 (MQ=255)            tACTCAACTACTCGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAaca                                                                                                                             >  3:380255/1‑134 (MQ=255)               tCAACTACTCGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCa                                                                                                                          <  3:325383/134‑1 (MQ=255)                cAACTACTCGTGACTCTTTCCATGGTGGGCGGAGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCAtt                                                                                                                        >  1:174096/1‑135 (MQ=255)                    tactCGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGt                                                                                                                    >  8:298228/1‑135 (MQ=255)                        cGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCTCAATGTTTCCACAAACGGTTCGCCTGCACCGCTGCCTCCGTAGAGATAAATCTAGTGCAGGCCACCAAACACCAGATCGTGATa                                                                                                                >  6:415925/1‑135 (MQ=255)                        cGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATa                                                                                                                >  3:404539/1‑135 (MQ=255)                          tGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAg                                                                                                               <  6:136604/134‑1 (MQ=255)                              tctTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTg                                                                                                         >  8:26268/1‑136 (MQ=255)                                   ccATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCAGCTGGCaa                                                                                                     >  6:208166/1‑135 (MQ=255)                                      tggtggGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACtt                                                                                                  <  6:158990/135‑1 (MQ=255)                                         tggGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTcc                                                                                                <  5:89546/134‑1 (MQ=255)                                             cGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATc                                                                                           >  6:106317/1‑135 (MQ=255)                                             cGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATc                                                                                           <  3:266884/135‑1 (MQ=255)                                             cGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATc                                                                                           >  1:354871/1‑135 (MQ=255)                                              ggTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCg                                                                                          >  5:100075/1‑135 (MQ=255)                                              ggTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGc                                                                                         >  7:446541/1‑136 (MQ=255)                                               gTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGc                                                                                         <  3:88176/135‑1 (MQ=255)                                                   cTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAACCACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTgg                                                                                     >  2:429001/1‑135 (MQ=255)                                                      cAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGcc                                                                                  >  2:16537/1‑135 (MQ=255)                                                        aGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATc                                                                               <  7:55453/136‑1 (MQ=255)                                                        aGCTCGCGTAACGGCTGCGCAATGTATCCACAAACGCTTCGCCTCACCCGCTGCCTCCGTAGAGATAAATCATGTTCTCGCCACCAAACACCATAAAGAGATAGCGCCTCGCAACGTCCTCATCGCTTGGCCCag                                                                                >  3:6796/1‑134 (MQ=255)                                                          cTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCgg                                                                             <  7:302550/136‑1 (MQ=255)                                                          cTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCg                                                                              <  5:236122/135‑1 (MQ=255)                                                           tGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCgg                                                                             <  1:222646/135‑1 (MQ=255)                                                            ggCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCgggg                                                                           <  8:409054/136‑1 (MQ=255)                                                                 aaCGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGccc                                                                      >  8:215458/1‑136 (MQ=255)                                                                 aaCGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGcc                                                                       >  3:80005/1‑135 (MQ=255)                                                                 aaCGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGcc                                                                       >  4:176038/1‑135 (MQ=255)                                                                 aaCGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGAGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGcc                                                                       >  2:30214/1‑135 (MQ=255)                                                                       tGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGAt                                                                 >  6:199665/1‑135 (MQ=255)                                                                                           ggTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCt                                             >  4:269133/1‑135 (MQ=255)                                                                                                 ccTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCCGATGCAACGTCAGCTTGTCGCCTTCGccc                                       >  2:49676/1‑135 (MQ=255)                                                                                                 ccTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGAcc                                       <  6:142284/135‑1 (MQ=255)                                                                                                      cccGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGa                                   <  5:199665/134‑1 (MQ=255)                                                                                                       ccGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACa                                <  8:149232/136‑1 (MQ=255)                                                                                                                   tAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTcc                     <  5:149831/135‑1 (MQ=255)                                                                                                                        aTAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAg                <  1:163339/135‑1 (MQ=255)                                                                                                                           aaTCTTGTGCAGGCCTCCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCTCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGCCCGCGCACATCGGCGTGTGCCCCAGGaa             >  5:138124/1‑135 (MQ=255)                                                                                                                           aaTCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGaa             >  4:288479/1‑135 (MQ=255)                                                                                                                           aaTCTTGTGCACGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGc                                                      >  4:4679/1‑94 (MQ=255)                                                                                                                           aaTCTTGTGCACGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGc                                                      <  3:10142/94‑1 (MQ=255)                                                                                                                           aaTCTTGTGCACGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGc                                                      >  4:10142/1‑94 (MQ=255)                                                                                                                           aaTCTTGTGCACGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGc                                                      <  3:4679/94‑1 (MQ=255)                                                                                                                             tCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGTAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACt           <  6:193090/135‑1 (MQ=255)                                                                                                                             tCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAAAATCGGCGTGTCCCACAGGAACt           <  3:441787/135‑1 (MQ=255)                                                                                                                                 gtgCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACTTTGc       >  1:425573/1‑135 (MQ=255)                                                                                                                                  tgCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACTTTGcc      <  8:446541/135‑1 (MQ=255)                                                                                                                                   gCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCACCTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGCTGCAACGTCAGCTTGTCGCCTTCGACCGCGCACATCGGCGTGTCCCGCAGGAACGTTGGCg     >  1:435540/1‑135 (MQ=255)                                                                                                                                   gCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGACCTTTGCCg     >  1:334283/1‑135 (MQ=255)                                                                                                                                   gCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACTTTGCCg     <  3:176038/135‑1 (MQ=255)                                                                                                                                   gCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACTTTGCCg     <  2:174096/135‑1 (MQ=255)                                                                                                                                     aGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACa                                <  2:287001/106‑1 (MQ=255)                                                                                                                                     aGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACa                                >  1:287001/1‑106 (MQ=255)                                                                                                                                     aGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTACCACAGGCACTTTGCCGtt   >  1:142394/1‑134 (MQ=255)                                                                                                                                     aGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGGCCCAACGGAACTGTGCCggc   >  4:33306/1‑133 (MQ=255)                                                                                                                                     aGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCACAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCTCAGGCACTTTGCCGTa   >  4:33310/1‑135 (MQ=255)                                                                                                                                     aGGCCACCAAACACCATTTCGACATAGCGGCTGCCAACTTCCTCATCGCATGGGCCATCGGGGAGCCCCAGATGCAACGACACCATGTCGCCTTCGACCGCGAACATCGGCGTCTCCAACAGGAAATTTGCCGTAc  >  8:263682/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                       TCCCTGGCAGTTACTCAACTACTCGTGACTCTTTCCATGGTGGGCGGTGACTGCAAGCTGGCGTAACGGCTGCGCAATGTTTCCACAAACGGTTCGCCTGCCCCGCTGCCTCCGTAGAGATAAATCTTGTGCAGGCCACCAAACACCATTTCGTGATAGCGGCTGGCAACTTCCTCATCGCTTGGGCCATCGGGGAGCCCCAGATGCAACGTCAGCTTGTCGCCTTCGACCGCGAACATCGGCGTGTCCCACAGGAACTTTGCCGTAC  >  KT2440/3891615‑3891882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑