Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,912,361 A→C 24.1% E833A (GAG→GCG)  mexB_2 → Multidrug resistance protein MexB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,912,3610AC24.1% 98.1 / 4.2 65E833A (GAG→GCG) mexB_2Multidrug resistance protein MexB
Reads supporting (aligned to +/- strand):  ref base A (13/34);  new base C (13/4);  total (26/39)
Fisher's exact test for biased strand distribution p-value = 1.06e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCGCTGCTGGT  >  KT2440/3912233‑3912493
                                                                                                                                 |                                                                                                                                    
cAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCTACAGCTCGGGCGCGGccct                                                                                                                                >  3:84560/1‑133 (MQ=255)
cAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGGCGATCTCAGGCGCGCCTGCCGCCGGCTACAGCGCGGGCGCGGCCAt                                                                                                                                >  5:104575/1‑135 (MQ=255)
  aGATGGGGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGGCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATgg                                                                                                                              <  5:201097/135‑1 (MQ=255)
    aTGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCGCAGGCGAGCCTGCCGCCGGCGACCGCTCGGGCGCGGCCCTGGcc                                                                                                                            >  2:397422/1‑135 (MQ=255)
        tGCCACGGGGCGCGTGCGTGCAGGCCAGGTGGGTCCGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGAGCTCAGGCGAGCCGGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGt                                                                                                                       <  8:208812/136‑1 (MQ=255)
         gCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTGGATCTCAGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGCGGCCCAGGCCGCAGt                                                                                                                       >  3:123121/1‑135 (MQ=255)
          ccACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTc                                                                                                                      <  4:187126/135‑1 (MQ=255)
          ccACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGGCGCTCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGCGGCCATGGCCGAAGTCg                                                                                                                     >  8:142151/1‑136 (MQ=255)
           cACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATGACAGGCGACCCTGCCGCCCGCTCCAGCTCGGGCGACGCGCAGGCCGAAGt                                                                                                                       >  2:332042/1‑133 (MQ=255)
                  cgcgTTCGGGCAGGCCAGGGGGGGCAGCGGCCCGGTGCAACGGACCCGCTACAACGGTTACCC‑GGCGGTGTCGAGCGCAGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCCGAAGTCGAGCGGCt                                                                                                              <  4:316255/135‑1 (MQ=255)
                     gTTGGTGCAGGCCTGGGGGGGCCGCGGCCCGGCTCAACAGTCCCGCAACAACGGTCACCC‑GGCGGTGTCGAGCTCCGGCGAGCCTGCCGCCGGCTACCGCGCGGGCGAGGCCAGGGCCGAAGCCGAGCGGCtggtg                                                                                                          <  8:162096/136‑1 (MQ=255)
                     gTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTGGATCTCACGCGAGCCTGACGCCCGCGACAGCTCGGGCGCGGCCATGGGCGAAGTCGAGCGGCgggt                                                                                                           >  1:276671/1‑135 (MQ=255)
                     gTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCCTGGCCGAAGTCGAGCGGCtggtg                                                                                                          >  8:401550/1‑136 (MQ=255)
                     gTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGGCGATCTCAGGCGCGCCGGCCGCCGGCGACCGCTCGGGCGCGGCCCTGGCCGCAGGCGGGCGGCtggg                                                                                                           >  4:233615/1‑134 (MQ=255)
                            cAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTACCCC‑GGCGGTGTCGATCTCCGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAg                                                                                                    <  6:2010/135‑1 (MQ=255)
                             aGGCCAGGGGGGGCAGCGGCCCGGCGCAACTGACCCGCTACACCGGTTCCCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCAGGGCCGAAGTCGAGCGGCTGGTGGCGCAg                                                                                                    <  6:238111/134‑1 (MQ=255)
                                ccAGGTGGGGCAGCGGCCGGGTGCACCTGACCCGCTACAACGGTTCCCG‑GGCGGGGTCGATCGCAGCCGCGCCCGCCGCCGGCAACAGCTCGGGGGAGGCCATGGCCTAAGTCGAGCGGCTGGGGGCGCAGCTAcc                                                                                               <  8:61872/136‑1 (MQ=255)
                                 cAGGTGGGTCAGCGGCCCGGGGCACCTGCCCCGCTACACCGGTTACCC‑GGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTAcc                                                                                               <  6:192580/135‑1 (MQ=255)
                                  aGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTAccgg                                                                                             >  7:87672/1‑136 (MQ=255)
                                     tGGGTCAGCGGCCCGGTGCACCTCACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTAccggccg                                                                                          <  8:100673/136‑1 (MQ=255)
                                      gggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTAccggccg                                                                                          <  3:87928/135‑1 (MQ=255)
                                       gtggcgCGGCCGGGTGAACCTGCCCGGATACAAGGTTTACGG‑GGGGGTGGCTCTCTCTGGCGTGCCGTCCGCCGGCTACCGCTCGGGCGCGGCCTGGGCGTAAGGCGAGCGGCTGGTGGCGCAGCTAccggccgg                                                                                         <  6:41949/130‑1 (MQ=255)
                                       gggtcccGGCCCGGGGCAAACGCACCGCGAACACGGGTACCCGGGGGGGGCCGAGCCCCGGCGCGCCGCCCGCGGGCTCCCGCGCGGGCGGGGCCTGGCCCCACGGCGCGCGGCTGGTGGCGCAGCTAccggccg                                                                                          <  2:231311/129‑1 (MQ=255)
                                       ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCGGCAGCTCGGGCGCGGCCATGGGCGCAGTCGCGCGGCGGGTGGGGCCGCTAccggccgg                                                                                         >  1:376218/1‑135 (MQ=255)
                                       ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACCGCGCGGGCGCGGCCCTGGCCGCAGTCGCGCGGCGGGTGGCGCAGCTAccggccgg                                                                                         >  5:275292/1‑135 (MQ=255)
                                       ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCGACAGCGCGGGCGAGGCCATGGCCGAAGTCGAGCGGCGGGTGGCGCAGCTAccggccgg                                                                                         >  5:233987/1‑135 (MQ=255)
                                         gccgCGGCCCGGGGCACCTCCCCGGCTACACGGGTCCCCG‑GGCGGGGGCGAGCTCCGGCGCGCGGGCCGCCGGCTACCGCGCGGGCGCGCCCAGGCCCGATGGCGCGCGGCTGGTGGCGCAGCTACCGGCCGGTa                                                                                       <  4:4535/132‑1 (MQ=255)
                                           cggggCCGGGTGAACCTGACCGGCTACACGGTTTCCCC‑GCCGGGGTCGAGCGCCGGCGAGCCGGCCGCCGGCTACAGCGGGGGCGCGGCCTTGGCCGATGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTAcc                                                                                     <  4:15077/132‑1 (MQ=255)
                                           aGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTGAGGCGAGCCTGCCGCCGGCTACAGCTCCGGCGAGGCCCAGGGCGAAGTCGCGCGGCTGGGGGCGCCGCTACCGGCCGGGAcc                                                                                     >  1:370431/1‑135 (MQ=255)
                                           aGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGGGGCTCTCAGGCGCGCCGGCCGCCGGCGACAGCGCGGGCGCGGCCCTGGCCGCAGTCGCGCGGCGGGTGGGGCAGCGAccggccggggcc                                                                                     >  3:4535/1‑131 (MQ=255)
                                            gCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTAcc                                                                                     <  4:171889/134‑1 (MQ=255)
                                              ggCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTc                                                                                 >  7:328591/1‑136 (MQ=255)
                                               gCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCt                                                                                <  8:87672/136‑1 (MQ=255)
                                                cccGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGCTCTCAGGCGCGCCTGCCGCCGGCTCCCGCGCGGGGGCGGCCCTGGCCGCAGTCGCGCGGCGGGGGGCGCCGCGACCGGCCGGGACCGGGCTg                                                                               >  7:338105/1‑136 (MQ=255)
                                                  cGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCGACAGCTCGGGCGCGGCCCTGGCCGAAGTCGAGCGGCGGGTGGCGCAGCTACCGGCCGGTACCgggcggg                                                                              >  7:396467/1‑130 (MQ=255)
                                                    gTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGCGGCCAAGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGaa                                                                            >  4:269914/1‑135 (MQ=255)
                                                      gCAACTGCCCCGCTACAACGGTTACCC‑GGGGGTGGCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATg                                                                          <  1:397422/135‑1 (MQ=255)
                                                         aCTGACCCGCTACAACGGTTACCC‑GGCGGGGTCGATCTCCGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGAc                                                                       <  1:137068/135‑1 (MQ=255)
                                                             aCCCGCTACAACGGTTACCC‑GGCGGTGTCGCTCTCAGGCGAGCCTGCCGCCGGCGACAGCGCGGGCGAGGCCATGGCCGAAGTAGCGCGGCTGGTGGCGCCGCTACCCGCCCGTACCGGGCTGGAATGGACCCGc                                                                   >  5:116907/1‑135 (MQ=255)
                                                             aCCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGcc                                                                  <  8:50549/136‑1 (MQ=255)
                                                             aCCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGcc                                                                  <  8:304516/136‑1 (MQ=255)
                                                              cccGCTACAACGGTTCCCC‑GGCGGTGTCGAGCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCAGGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGcc                                                                  <  4:20350/135‑1 (MQ=255)
                                                                cGCTACAACGGTTACCC‑GGCGGTGTCGATCTCCGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTg                                                                <  6:104575/135‑1 (MQ=255)
                                                                      aaCGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCAc                                                            <  6:180910/133‑1 (MQ=255)
                                                                             aCCC‑GGCGGTGGCGATCTCAGGCGCGCCGGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCg                                                  <  7:409175/136‑1 (MQ=255)
                                                                              ccc‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCg                                                  <  4:78758/135‑1 (MQ=255)
                                                                              ccc‑GGCGGGGTCGAGCGCTGGCGGGCCTGCCGCCGGCTCCAGCTCGGGCTCGGCCTTGGCCGACGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCg                                                  <  3:4673/135‑1 (MQ=255)
                                                                               cc‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCg                                                  <  1:63514/134‑1 (MQ=255)
                                                                                c‑ggcggTGTCGATCTCAGGCGAGCCAGCCGCCGGCTACAGCTCGGGCGAGGCCATGGGCGAAGTGGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTGTGGAATGGGCCGGGCTGTCACTGCAGGCGCGGc                                                >  4:140036/1‑135 (MQ=255)
                                                                                  ggcggGGGCGAGCTCCGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCt                                               <  6:186042/135‑1 (MQ=255)
                                                                                    cggTGTCGATCTCCGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTc                                            <  7:342518/136‑1 (MQ=255)
                                                                                       tggcGATCTCACGCGAGCCTGCCGCCGGGTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCCGCTACCCGCCGGTACCGGTCGGGAATGGCCCCGCCTGTCACAGCATGAGCGGCTGTCCgg                                         >  8:413786/4‑136 (MQ=255)
                                                                                             tcgccggCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGcc                                    <  3:279262/130‑1 (MQ=255)
                                                                                                 cggCGCGCCTGCCGCCGGCTACCGCGCGGGCGAGGCCAGGGCCGAAGGCGCGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGcc                               <  8:82161/135‑1 (MQ=255)
                                                                                                    cGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGccccc                            >  7:238412/1‑136 (MQ=255)
                                                                                                           gccgccGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGcc                                    <  6:99881/121‑1 (MQ=255)
                                                                                                           gccgccGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGcc                                    >  5:99881/1‑121 (MQ=255)
                                                                                                             cgccgGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATg                   <  8:238412/136‑1 (MQ=255)
                                                                                                                    tACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTg             >  3:243417/1‑135 (MQ=255)
                                                                                                                       cgcgcGGGCGAGGCCAGGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGt            <  2:194415/129‑1 (MQ=255)
                                                                                                                           cGGGCGAGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCgctgc      <  2:358933/135‑1 (MQ=255)
                                                                                                                             ggCGAGGCCATGGCCGAAGTGGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCGCTGCtgg   <  8:396467/136‑1 (MQ=255)
                                                                                                                             ggCGAGGCCATGGCCGAAGTCGTGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCgctgc      <  1:332042/133‑1 (MQ=255)
                                                                                                                              gCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCGCTGCtgg   >  5:6931/1‑135 (MQ=255)
                                                                                                                               cGAGGCCATGGCCGATTTCGAGCGGCGGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCGCTGCtggt  <  2:113958/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
CAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCC‑GGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGTCCGGTAGCCAGGCCCCCCTGCTGATGGCGCTGTCGCTGCTGGT  >  KT2440/3912233‑3912493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑