Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,934,892 A→C 35.9% V100G (GTG→GGG)  CJ019_03719 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,934,8920AC35.9% 47.9 / 9.8 42V100G (GTG→GGG) CJ019_03719hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (3/22);  new base C (13/3);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 1.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCGTAG  >  KT2440/3934760‑3935023
                                                                                                                                    |                                                                                                                                   
cGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCacac                                                                                                                                  <  7:10320/136‑1 (MQ=255)
   tgctgACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGcccccccccccc                                                                                                                                >  2:172446/1‑135 (MQ=255)
   tgctgACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGACCACGCCGCCCGcccccccc                                                                                                                                >  5:112595/1‑135 (MQ=255)
         ccGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCCCCGCGCTTGATGTCTGCCACGGCCCCCCCCCCAcccgccggg                                                                                                                          >  1:189932/1‑129 (MQ=255)
             aaTCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCCCCCCAACCACcggcggcgg                                                                                                                     >  7:24908/1‑136 (MQ=255)
                cagcagcagCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCCCCCCCCCCGCGggcggcggcgc                                                                                                                  >  8:203978/1‑134 (MQ=255)
                 agcagcagcACCCCCAGGTATGAAAAGCCGCCGTTGGCCTTACCATTCCCCGAAGAGGCGGCCAGCCCGCGCCCTCCCTGGGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCCCACCCTCGGGCGGCGGCAc                                                                                                                  <  5:402927/135‑1 (MQ=255)
                            ccccAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCATCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCACACCCAACGGCGGCCGCCCCACCAGCCAgg                                                                                                       >  5:167754/1‑135 (MQ=255)
                                        aaaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCCCGCCGCCCGCCCCCCCCCCCGGCGGCGGCCCCCGCTGCCCCGCATCGCGgcgcg                                                                                           >  3:125250/1‑134 (MQ=255)
                                        aaaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCCCCCCCCCCCCCGGCGGCGGCACCCCCAGCCCCGCCTCGCTgcgcgc                                                                                          >  7:269815/1‑134 (MQ=255)
                                        aaaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCCCCCCACCCGCGGGCGGCGGCCCCCGCTGCCAGGCATCGCGGCGCt                                                                                           >  6:229161/1‑135 (MQ=255)
                                        aaaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCGCCCCCCCCCCCGGCGGCGGCCCCCCCTGCCAGGCATCGCAgcgcg                                                                                           >  3:146000/1‑134 (MQ=255)
                                         aaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCCCCCGCGGGCGGCGGCCCCCCCGGCCACGCCTCGCTgcgcgc                                                                                          >  6:97482/1‑133 (MQ=255)
                                          aGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCCCCACCCCCTCGGGCGGCGGCCCCACCGGCCCGGCATAGCTGcgcgcg                                                                                         >  6:202900/1‑135 (MQ=255)
                                               ccgTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCCCCCTCGGGCGGCGGCCCCAGCGGCCCGGCATCCCTGCGCGCgggggtc                                                                                   >  8:359909/1‑131 (MQ=255)
                                                                 aGCCAAGAGGCGCCCAGCACGCCCCCCCCCGGGGGCCCCGGTTTGTAGGGCGGCCCCCCCGCCCCCCACCCCCGGGGGCGGGGGCTCCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgtgtgt                                                                   <  5:182133/134‑1 (MQ=255)
                                                                  gCGACGAGGCTGCCATCACGCGCCCGCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTg                                                                 <  3:238389/135‑1 (MQ=255)
                                                                  gCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCCCCACCCCCTCGGGCGGCGGGCCCAGCTGCCCCGCATGGCTGCGCTGGGTTGTCGGGGGGCCCGGGGGGTTg                                                                 >  4:186177/1‑135 (MQ=255)
                                                                      agagGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCACCCCCGGGGGGCGGCCCCCGCTGCCCCGCCTCGCAGGGCTGGGGGATCGGGGCGACCGGGGTGtttggca                                                             >  2:286016/1‑130 (MQ=255)
                                                                        agGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATC‑GGTCGACCGGTGTGTTGCGCAGGt                                                          <  4:202332/135‑1 (MQ=255)
                                                                         ggCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCCCCCACGGGGGGCGGGCCCCGCGGCCCCGCATACCGGCGCGCGGGGCTCGGGGGGCCCGGGGTGTTGGGCAgg                                                           >  2:10784/1‑134 (MQ=255)
                                                                                aTCACGCGCCCTCCGTGTGGCACCGCGCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACgttgt                                                  <  8:385507/136‑1 (MQ=255)
                                                                                aTCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCACCCACCGGCGGCGGCCACAGCAGCCACGCCTAGCAGCGCGCGGGGAGCGGGTCGGCCGGGGTGGTGGGCAGGTTACggtg                                                   >  4:161164/1‑135 (MQ=255)
                                                                                 tCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACgttgt                                                  <  2:244610/135‑1 (MQ=255)
                                                                                                          tctTGATGTCGGCCACGCCCCCCCCCCCCCCCACCGGCGGCGGCCCCCCCAGCCCCGCCTAGCGGCGCTCGGGGGTAGGGTGGGCCCGGGTGTTTGGGCGGTTACGTTTGTGCACCACTTGTTGCAGCGacgcgg                         >  6:361858/1‑130 (MQ=255)
                                                                                                           ctTGATGTCGCCCACGCCGCCGGCCTCAGCCTGGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTGGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCggg                        <  4:437632/135‑1 (MQ=255)
                                                                                                               aggTCGGCCCCGCCGCCCCCAACACCCGGGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  7:392998/134‑1 (MQ=255)
                                                                                                               aggTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  7:216801/134‑1 (MQ=255)
                                                                                                               aTGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTa                     <  3:139710/134‑1 (MQ=255)
                                                                                                                tggcGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  6:87866/132‑1 (MQ=255)
                                                                                                                ggTCGGCCAGGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  2:35927/134‑1 (MQ=255)
                                                                                                                 gTCGGCCACGCCGCCCGCCACACCCTCGGGGGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  2:189932/134‑1 (MQ=255)
                                                                                                                   cGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGcc               <  8:1807/136‑1 (MQ=255)
                                                                                                                     gCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  7:359909/136‑1 (MQ=255)
                                                                                                                      ccccgccgcCCGCCACACCCTCGGGGGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  1:217896/132‑1 (MQ=255)
                                                                                                                      ccccgccccCCCCCACCCCCGCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  1:408051/132‑1 (MQ=255)
                                                                                                                      ccACGCCGCCCGCCCCACCGTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  2:170047/135‑1 (MQ=255)
                                                                                                                      ccACGCCGCCCGCCCCACCGCCGGCCGGCGGCACCAGCTGCCACGCATCGCTGCGCTCGGTGACCGGGTCGACCGGTGGGTTGCGCAGGTAACGTTGTTCCACCAGATGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  6:167754/135‑1 (MQ=255)
                                                                                                                      ccACGCCGCCCGCCACACCCTCTGGCGGAGGCACCAGCTGCCAGGAATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  4:259535/135‑1 (MQ=255)
                                                                                                                      ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:97482/135‑1 (MQ=255)
                                                                                                                      ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:229161/135‑1 (MQ=255)
                                                                                                                           ccgcccgcCACACCCGCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCa        <  2:155855/135‑1 (MQ=255)
                                                                                                                                cgccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCgtag  <  7:341517/136‑1 (MQ=255)
                                                                                                                                cgccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCgtag  <  8:341714/136‑1 (MQ=255)
                                                                                                                                cgccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGGCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCgta   <  4:323970/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCGTAG  >  KT2440/3934760‑3935023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑