| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,135,612 | T→G | 46.2% | W34G (TGG→GGG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,135,612 | 0 | T | G | 46.2% | 3.5 / 9.1 | 38 | W34G (TGG→GGG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (9/7); new base G (7/9); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTC > KT2440/4135483‑4135738 | ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCACCCCGGGgccc > 8:307441/1‑134 (MQ=255) cTCGCCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGATCGTTTCCAGATGTGCATCGACGGCCAAAGGCGCGATGCCCAGCGCGTCTAGACCTTCGACCGCCGCCACCCGGCCACCCCCCCGGCCAggggcc > 2:180975/1‑132 (MQ=255) ccGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACt > 4:95317/1‑135 (MQ=255) ccGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCCACCCGGCCACCGCCCCCCCCCGGGCGCCCCAGCCCgc > 1:170712/1‑134 (MQ=255) aGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCCGGGCGCACCTGCCCGCCGCCGAt > 7:208184/1‑136 (MQ=255) ggATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa < 2:292369/135‑1 (MQ=255) ggATGGTTGCTTGACCCTCGTGCGTTCCCATATGTGCAACGACGGCCAATGGCGCGTGGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGa < 6:1943/135‑1 (MQ=255) gttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCAGGGCGCCACTGCCCGCCCCCGATGCAGcc > 4:438101/1‑134 (MQ=255) tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACAGCCCGCCGCCGATGAATcccaag > 7:147755/1‑132 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCCCCGGGGCGCCCCTCCCCCCCGCCCACCAAGCCCAAGTGGAg > 7:80024/1‑136 (MQ=255) gttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACGGCCCGCCGCCGAGGAAGCCGCTGTGGGGCTGGcc > 1:91552/1‑135 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGCCGCCGCTGCAGCCGATGTGGCGCTGGCCGGTGAGgcc > 8:446469/1‑136 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACAGCCCGCCGCCCCTGAAGCCCCTGTGGAGCTGGCCGTTCAGgc > 2:6971/1‑135 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACAGCCCGACGCCGATGAAGCCGCTGTGGAGCTGGCCGTTCAGgc > 6:339277/1‑135 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCAGGGCGCCACTGCCCGCCCCCCAAGGAGCCGATGTGGAGCTGGCCGATAAGgcc > 8:295165/1‑136 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATG‑CCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCACCTGCCCCACGCCGATGCAGCCGCTGTGGAGCTGGCCGTTCAGgcc > 4:425510/1‑135 (MQ=255) aggcgCATCCCCGGCCACTGGCGCGCGGCCCAGAGCGGCAAGCCCTACGACCCCCTCACCCCGGCCCCCGCCCCGGCCGGGCCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc < 7:107368/132‑1 (MQ=255) tCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCCGCCACGGCGCCCCTGCCCCCCGCCGCTGAAGCCGATGTGGGGCGGGCGGTTCTGGCCGCCCCGCGAGc > 8:21796/1‑136 (MQ=255) aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGGGCCCCTGCCCGCCGCCGCTGAAGCCGCTGTGGAGCTGGGCGGTGAGGCCGCCCCGCGGGCCGTCGCCa > 1:247214/1‑135 (MQ=255) ccgcgAGGCCCCGCGCGGCAAGACCTTCGCCAGCCTCACCCCGCCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 8:233975/135‑1 (MQ=255) cgcgCTGCCCACCGCGGCACGACCAGCGACCGCCTCACCCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGCCGCCGATGAAGCCGAGGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 6:275076/135‑1 (MQ=255) gATGCCCCGAGCGGCAAGCCCTTCGACCGCCGCCACCCGCCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCg < 5:354613/135‑1 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGCTGTGGCGCTGGCCGTTCAGGCCGCCCCGCGGGCCGTCGCCAGCAAGGCGTg > 5:223691/1‑135 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCGCCGATGACTCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTGGCCAGCAAGGCGTgg > 7:322178/1‑136 (MQ=255) cAGAGCGCCAAGACCTTCGACAGCCTCACCCCCGCCACCCCCCAGGCCGGCGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 1:157485/135‑1 (MQ=255) gcgCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 2:247214/133‑1 (MQ=255) aaGCCCTTCGACAGCCTCAACCCGGCCACACCCCCGGCCGGGGCCCAACAGCCCGCCGCCGAGGACGGCGCTGTGGAGCGGGCCGTTCAGGCCCCCCAGCGGTCCTGCGCCCGCCAGGCGTGGCGCGCCGTCAcc > 6:112932/1‑135 (MQ=255) aCAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgccc < 4:278921/135‑1 (MQ=255) cgCCTCACCCCGCCCCCCGCCCAGCCCGGGGCGCAACTGCCCGACGCCGATGCAGCCGAGGTGGAGCTGGCCGGTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgcccgc < 5:131529/134‑1 (MQ=255) gCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 2:250779/135‑1 (MQ=255) cAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGGGCTGGCCGTTCAGGCCGCCCAGCGTGGCGTCGACAGCAAGGCGGGGCGCAGCATAACCCCTACCCCCCGCGGCCAg > 8:230744/1‑136 (MQ=255) gcGGCCCCCGCCCAGGCGGGGCCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg < 5:304998/134‑1 (MQ=255) tgCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCGTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 2:170712/134‑1 (MQ=255) ggCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 6:340284/135‑1 (MQ=255) ccccGCCCCGGCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGcgt < 8:80024/134‑1 (MQ=255) aCCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGCCGTCGCCAGCAAGGCGTGGCGCCGCATCACCGCGACCGCCCGCGGCCAGCGGCTGcgt > 2:59710/1‑135 (MQ=255) aCCGCCCCGGCCTGGGCGCAACTGCCCCCCGCCGCGGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGGCGGGGACAGCAAGGCGTGGCGCCGCCTCACCGCTACCGCCCCCGGCCAgcggcggcgg > 2:15706/1‑134 (MQ=255) cccTGGCCTGGGCGCAACTGCCCGACGCCGATGAATCCGTTGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCTCTACCGCCCGCGGCAAGCTGCTGCgtcgtc < 4:382722/135‑1 (MQ=255) | CCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTC > KT2440/4135483‑4135738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |