Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,150,909 G→T 8.8% V205V (GTC→GTA)  cmpR_5 ← HTH‑type transcriptional activator CmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,150,9090GT 8.8% 142.6 / 2.6 52V205V (GTC→GTA) cmpR_5HTH‑type transcriptional activator CmpR
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base T (3/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCATGACGAATGGACATCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCGCCACGATCTCGTCCTCACGCCA  >  KT2440/4150776‑4151043
                                                                                                                                     |                                                                                                                                      
ccTCATGACGAATGGACATCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTACCCCTTCAACGCCCGCGAACTCCAGCGCCACACGCAGCCCCACGCCATCACGGGCAACCGCCCGCTcga                                                                                                                                       >  1:232659/1‑135 (MQ=255)
       aCGAATGGACATCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTg                                                                                                                                >  3:263304/1‑135 (MQ=255)
        cGAATGGACATCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCGTTTACCCCTTCAACGCCCGCGCGCTCCAGCGCCACCCGCATCGCCACGCCATCCCGGGCAAACGCCCGCTCGCCGATCTGc                                                                                                                               >  4:234114/1‑135 (MQ=255)
        cGAATGGACATAGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTATTTTACCCCTTCAACGCCCCCGAACTCCACCGCCACACGCATCGCCACGCCATCAACGGCAAACGCCCGCACGCCGATCTGc                                                                                                                               >  3:266985/1‑135 (MQ=255)
             ggACATCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGCCCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCCCACGCCTCGCCACGCCATCACGGGCACACGCCCGCTCGACGATCTGCCGcac                                                                                                                          >  3:106185/1‑135 (MQ=255)
                 aTCGCCGACCCAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTCCCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCg                                                                                                                     <  8:170164/136‑1 (MQ=255)
                 aTCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACAccc                                                                                                                      >  1:320828/1‑135 (MQ=255)
                  gcGCCGACACAAAGCCAACCCCCATCCCGGCCCGGCCCGCCTCTTTTACCCCTTCAACGCCCGCGAGGTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCg                                                                                                                     <  2:234020/134‑1 (MQ=255)
                            aaaGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTACCCCTTCACCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTc                                                                                                           <  2:45031/135‑1 (MQ=255)
                            aaaGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTAACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTc                                                                                                           <  1:166316/135‑1 (MQ=255)
                            aaaGCCAACCCCCAACCCGGCCCGGACCGCCTCTTTACCCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTc                                                                                                           <  1:448234/135‑1 (MQ=255)
                             acgCCACCCCCCATCCCGGCCCGGACGCCCTCTTTTACCCCTTCAACGCCCGCGAGCTCCAGCGCCACACGCATCGCCACGCCAGCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCa                                                                                                          <  1:95535/133‑1 (MQ=255)
                                                  cggACCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGgcg                                                                                     <  3:336872/135‑1 (MQ=255)
                                                  cggACCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCGGTCCCGCAGTACCCACAGGTAGgcg                                                                                     >  1:401118/1‑135 (MQ=255)
                                                      ccgcagctTTTACCCCTACAACGCCGGCTAGCTCCCCCGCCACCCGCATCGCCCCGCCATCAAGGTAAAACGCCCGCTCGACGATCTGCCGCCCACCCGAGCCGCTTGCACGCAGTACCAACGGGTTGGCGCCGa                                                                                 <  4:266985/129‑1 (MQ=255)
                                                       cGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGGAGGCGCCgcg                                                                                >  2:263930/1‑133 (MQ=255)
                                                        gCCTCTTTTACCCCTTCAACCCCCGCGATCACCAGCGCCCCACGCCTCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACCCAGTACCAACGCGTAcgcgccgcgc                                                                               >  2:381821/1‑135 (MQ=255)
                                                            ctTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGccccg                                                                           >  2:290154/1‑135 (MQ=255)
                                                                tACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCgccgcc                                                                         <  4:263304/133‑1 (MQ=255)
                                                                aaCCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCACCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAg                                                                       <  7:423548/134‑1 (MQ=255)
                                                                          ccGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGCTCTGCCGCCCACCCGAGCCGCGTTAACGCAGTACCCAAGGGGAGTCGCCGAGCCCCGCCCGCCTCGGCTgg                                                             >  2:163128/2‑135 (MQ=255)
                                                                          aCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCTACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTctgctg                                                              >  4:436429/1‑134 (MQ=255)
                                                                          aCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCTACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTgg                                                             >  4:429266/1‑135 (MQ=255)
                                                                          aCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCGCACCCGAGCCGCTTTCACGCAGTACCAACGGGTCGGCGCCGAGCGCCGCCCGCGTCTGCTGGt                                                            >  8:309451/1‑136 (MQ=255)
                                                                          aCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACCCCCGCGCCGCGTTCACGCAGTCCCAACGGGTAGGCCCCGAGCGCCGCCAGCGTCTGCTgg                                                             >  2:101702/1‑135 (MQ=255)
                                                                          aCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTgg                                                             >  3:60840/1‑135 (MQ=255)
                                                                          aCGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACAAACGGGTAAGCGCCGAGCGCCGCCAGCGTCtggtgg                                                             >  2:351502/1‑135 (MQ=255)
                                                                                  gATCTCCAGCGCCACCCTCATCGCCACGCCATCACGGGCAAACGCCCTCTCTACGATCTGCCGCACACCCGAGCCGCTTTAACGCAGGTCCACCGGGTTGGCGCCTCGCGCCGCCAGCGTCAGCTGGGCAcggcc                                                     >  6:135498/1‑135 (MQ=255)
                                                                                   aTCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGCCGATCTGCCGCACACCCGAGCCGCTTTCCCGCAGTACCACCGGGTAGGCGCCGCGCGCCCCCAGCCTCTGCTGGTCActgccgg                                                   >  7:339206/1‑134 (MQ=255)
                                                                                        cAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAg                                               <  2:320828/135‑1 (MQ=255)
                                                                                        cAGCCCCCCCCGCATCCCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAg                                               <  2:396574/135‑1 (MQ=255)
                                                                                         aGCGCCACCCGCATCGCCACGCCATCACGGGCAAACTCCCGCTCGACGATCTGCCGCACCCCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCActgcc                                                     <  5:71789/128‑1 (MQ=255)
                                                                                           cgcCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCCCGCAGGACCAACGGGTAGGCGCCGCGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCgg                                            >  4:345986/1‑135 (MQ=255)
                                                                                              cacaCGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTAc                                                                                                  >  3:285732/1‑78 (MQ=255)
                                                                                              cacaCGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTAc                                                                                                  <  4:285732/78‑1 (MQ=255)
                                                                                               acacGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGc                                        <  1:259259/135‑1 (MQ=255)
                                                                                                   gCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCCGCGTCTGCTGGTCACTGCCTGCCAGCGGATGccccc                                    >  4:150091/1‑133 (MQ=255)
                                                                                                    cATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGTTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACg                                   <  6:200475/135‑1 (MQ=255)
                                                                                                        gccacgccaTCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCCCTGCCTGCCAGCGGCTGCCCCCGGGGcc                              >  8:273134/1‑135 (MQ=255)
                                                                                                        gccacgccaTCAAGGGCAAACGCCCGCTCGACGCTCTGCCGCACACCCGAGCCGCTTTCACCCAGTACCACCCGCTACGCGCCGAGCGCCGCCAGCCACAGCTGGTCACTGCCGGCCAGCGGATGCCGCCGCGGCa                              >  7:119055/1‑136 (MQ=255)
                                                                                                              ccaTCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATc                         <  5:76743/135‑1 (MQ=255)
                                                                                                               caTCACGGGCAAACGCCCGCTCTACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCg                        <  3:429266/135‑1 (MQ=255)
                                                                                                               caTCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCg                        <  4:190691/135‑1 (MQ=255)
                                                                                                               caTCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGGAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCg                        <  1:263930/135‑1 (MQ=255)
                                                                                                               aaTCACGGGCAAACGCCCGCTCTACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCg                        <  3:436429/134‑1 (MQ=255)
                                                                                                                aTCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCGGGTCACTGCCTGCCAGCGGCTGCCCCCGGGGCACTATCGc                       >  1:355031/1‑135 (MQ=255)
                                                                                                                aTCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCGTTCCCGCCGGACCAACGGGTAGGCGCCGAGCGCCGCCAGCGGCTGCGGGGCCCGGCCGGCCAGCGGCTGCCCCCGGGGCCCGATCGc                       >  2:413234/1‑135 (MQ=255)
                                                                                                                  cACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCGTTGACCCAGTAACAACGGGTAGGCGCCGCGCGCCGCCAGCCTCAGATGGTGACTTCCTGCCAGCGGAAGCCCACCGGGCACAATCGccc                     >  5:399716/1‑134 (MQ=255)
                                                                                                                    cGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCAc                                                                                                         <  4:406777/49‑1 (MQ=255)
                                                                                                                    cGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCAc                                                                                                         >  3:406777/1‑49 (MQ=255)
                                                                                                                      ggCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCGCCACGAt                 <  1:418417/135‑1 (MQ=255)
                                                                                                                           aCGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCGCCACGATCTCGt            <  4:60840/135‑1 (MQ=255)
                                                                                                                                 gCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCGCCACGATCTCGTCCTCAc      <  6:142995/135‑1 (MQ=255)
                                                                                                                                     gacgaTCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCGCCACGATCTCGTCCTCACGCCa  >  4:191899/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CCTCATGACGAATGGACATCGCCGACACAAAGCCAACCCCCATCCCGGCCCGGACCGCCTCTTTTACCCCTTCAACGCCCGCGATCTCCAGCGCCACACGCATCGCCACGCCATCACGGGCAAACGCCCGCTCGACGATCTGCCGCACACCCGAGCCGCTTTCACGCAGTACCAACGGGTAGGCGCCGAGCGCCGCCAGCGTCTGCTGGTCACTGCCTGCCAGCGGATGCCCACGGGGCACTATCGCCACGATCTCGTCCTCACGCCA  >  KT2440/4150776‑4151043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑