Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,243,006 G→A 5.1% A222A (GCC→GCT)  FCS1 ← Trans‑feruloyl‑CoA synthase FCS1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,243,0060GA 5.1% 229.9 / 3.3 79A222A (GCC→GCT) FCS1Trans‑feruloyl‑CoA synthase FCS1
Reads supporting (aligned to +/- strand):  ref base G (35/39);  new base A (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

AGGCTGTCGTACAACGCATCGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAGGCCAC  >  KT2440/4242872‑4243140
                                                                                                                                      |                                                                                                                                      
aGGCTGTCGTACAACGCATCGGAGCCGGACAACGAGCTGGGGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCgg                                                                                                                                        <  5:28498/135‑1 (MQ=255)
     gTCGTACAACGCATCGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCaaa                                                                                                                                   >  2:83971/1‑135 (MQ=255)
       cGTATAACCCATCGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGc                                                                                                                                 <  2:417102/135‑1 (MQ=255)
                 aTCGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCCCGttc                                                                                                                      >  7:22261/1‑136 (MQ=255)
                 aTCGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCAGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGtt                                                                                                                       >  2:119558/1‑135 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCCCGttct                                                                                                                     >  3:60040/1‑135 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttct                                                                                                                     <  4:153347/135‑1 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttct                                                                                                                     >  5:69868/1‑135 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttct                                                                                                                     <  5:388632/135‑1 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttct                                                                                                                     >  4:168431/1‑135 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttct                                                                                                                     >  3:262749/1‑135 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttct                                                                                                                     <  2:47765/135‑1 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTAAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGttcg                                                                                                                     >  4:317310/1‑134 (MQ=255)
                   cGGAGCCGGACAACGAGCTGGTGTGGCGGAGCGCCAGGGCAGCGCCGATCTGCGAAACTCCCGTCGTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGTCGCGGGCAAAGCCCGGCACGttct                                                                                                                     <  4:153343/135‑1 (MQ=255)
                      aGCCGGACATCGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCTGGCAAAGCCCGGCACGTTCTTCa                                                                                                                  <  4:252484/135‑1 (MQ=255)
                         cGGACAACGAGCTGGTGGGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGcc                                                                                                              <  8:388928/136‑1 (MQ=255)
                          ggACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTGAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCt                                                                                                             <  8:294480/136‑1 (MQ=255)
                          ggACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACTTTCTTCAAGcc                                                                                                              <  5:182129/135‑1 (MQ=255)
                           gACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGATGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCTGCCCGTTCTTCAACCCtt                                                                                                            >  7:190420/1‑136 (MQ=255)
                                cGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAg                                                                                                        <  4:215460/135‑1 (MQ=255)
                                   gCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCgg                                                                                                                             >  8:257249/1‑111 (MQ=255)
                                   gCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCgg                                                                                                                             <  7:257249/111‑1 (MQ=255)
                                   gCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGGTCTTCAAGCCTTCCAGGTg                                                                                                     >  6:364121/1‑135 (MQ=255)
                                       gtgtGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGGGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGc                                                                                                <  8:376866/136‑1 (MQ=255)
                                        tgtgGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGc                                                                                                <  1:110442/135‑1 (MQ=255)
                                         gtgGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGc                                                                                                >  3:140197/1‑134 (MQ=255)
                                         gtgGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGc                                                                                                >  4:192430/1‑134 (MQ=255)
                                               gAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCCCGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGc                                                                                         >  4:363596/1‑135 (MQ=255)
                                               gAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGc                                                                                         >  6:198430/1‑135 (MQ=255)
                                                    ccAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTGCCAGGTGCAGGCCGATGGCGGTTa                                                                                    >  5:64559/1‑135 (MQ=255)
                                                    ccAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCGTCCAGGTGCAGGCCGATGGCGGTTa                                                                                    >  1:352628/1‑135 (MQ=255)
                                                     cAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTAc                                                                                   >  5:393506/1‑135 (MQ=255)
                                                       gTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCCAAGCCCGGCACGGTCTTCAAGCCTTCCAGGTGCCGGCCGCTGGCGGTTAccc                                                                                 >  5:143430/1‑135 (MQ=255)
                                                         tCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGt                                                                               <  4:14899/135‑1 (MQ=255)
                                                               ccGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGgtcg                                                                         <  1:182598/135‑1 (MQ=255)
                                                                     tGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCTCGGGCAAACCCCGGCACGGTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTAACCGTGGGTCGTCGa                                                                     >  5:12499/1‑133 (MQ=255)
                                                                        gAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGagca                                                                  <  4:115761/133‑1 (MQ=255)
                                                                        gAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGagc                                                                   <  4:189399/132‑1 (MQ=255)
                                                                        gAAACGCCCGTCTTCAATGCAATGAGCGGCGGGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGGTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGagca                                                                  <  1:390789/133‑1 (MQ=255)
                                                                             gCCCGTCTTGAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCAcatcc                                                          <  8:160924/136‑1 (MQ=255)
                                                                               ccGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGc                                                                                         >  4:181981/1‑103 (MQ=255)
                                                                               ccGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGc                                                                                         <  3:181981/103‑1 (MQ=255)
                                                                               cagTCTACAATGAAATGATCGGCGTGCCCTGCTGCAGCGCCTTCCAAGCGGCGAGTGCAAACCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCCATGGCGGTTACCCGTGGGTCGTCGAGCAGCAcatccat                                                        >  8:299966/3‑136 (MQ=255)
                                                                                 gTCTTCAATGCCATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCACGCCTTCCAGGTGCAGGCCGTTGGCGGTGACCCGTGGGTCGTCGAGCAGCACATCCATCa                                                      <  8:400611/136‑1 (MQ=255)
                                                                                  tCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCa                                                      <  3:121174/135‑1 (MQ=255)
                                                                                  tCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCa                                                      <  3:91320/135‑1 (MQ=255)
                                                                                    ttCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCCGc                                                    >  3:381230/1‑135 (MQ=255)
                                                                                    ttCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGc                                                    >  3:416733/1‑135 (MQ=255)
                                                                                    ttCAAAGCAATCATCGGCCTGCACTGCTACAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCCCCACGATATTCAACCCATACACGTTCAGGCCGATGGACGTTACCCCTTGTTAGTCGAGCAGCACATCCCTCAg                                                     >  5:410905/1‑134 (MQ=255)
                                                                                       aatgcaatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCt                                                   <  1:83971/133‑1 (MQ=255)
                                                                                        atgcaatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCGTCCAGGTGCAGGCCGCTGGCGGTGACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCg                                                 <  3:137656/134‑1 (MQ=255)
                                                                                          gcaatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGAGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGcc                                              >  6:301791/1‑135 (MQ=255)
                                                                                          gcaatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGAGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGcc                                              >  6:307613/1‑135 (MQ=255)
                                                                                            aatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAATCCCGGCACGGTCTTCAAAACTTCCAGGTGCAGGCCGAAGGAGGTTACCCTGTGGTCGTCAAGCAGCACATCCACCAGCTCGGCcaca                                           >  8:267256/1‑136 (MQ=255)
                                                                                            aatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCcaca                                           >  8:231165/1‑136 (MQ=255)
                                                                                            aatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCcac                                            <  5:121501/135‑1 (MQ=255)
                                                                                            aatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCca                                             <  3:96308/134‑1 (MQ=255)
                                                                                            aatgATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGAGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCcac                                            >  6:314581/1‑135 (MQ=255)
                                                                                                 tCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCCCGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCcccaccca                                       >  5:73789/1‑135 (MQ=255)
                                                                                                 tCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACCTCCATCAGCTCGGCCACCCCCa                                       >  1:137473/1‑135 (MQ=255)
                                                                                                      gTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCCAAGCCCGGCACCTTCTTCCAGCCGTGCAGGTGCAGGCCGATGGCGGGTACCCGTGGGTCGTCGCGCCGCCCATCCATCAGCTCGGCCACACCCAGTTgc                                  >  4:416267/1‑135 (MQ=255)
                                                                                                                gcAGCGCCTTGCTAGCGGCGCGGGCAATGCCCGGCACGTTCTTCAAGGCTTCCAGGTGCAGGCCGTTGGCGGTTACGCGTGGGTCGTCGAGCAGCACATGCATCAGCTGGGGCTCACCCAGTTGCGCCTGGTTGcc                       <  7:196971/136‑1 (MQ=255)
                                                                                                                gcAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGcc                       <  8:407608/136‑1 (MQ=255)
                                                                                                                 cAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTGCAGGTGCAGGCCGATGGCGGTTACCCGTGGGGCGTCGGGCGGCACATCCATCCGCTCGGCCACCCCCCGTTGCGCCTGGTTGcc                       >  5:328711/1‑135 (MQ=255)
                                                                                                                     gcCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgaccg                  <  7:41832/136‑1 (MQ=255)
                                                                                                                     gcCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgacc                   <  6:280407/135‑1 (MQ=255)
                                                                                                                      ccTTGCAAGCGGCGCGGGCATAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgaccg                  <  6:12499/135‑1 (MQ=255)
                                                                                                                      ccTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAATCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCTAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgac                    >  1:15336/1‑133 (MQ=255)
                                                                                                                      ccTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgaccg                  <  6:69868/135‑1 (MQ=255)
                                                                                                                      ccTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgaccg                  <  4:140197/135‑1 (MQ=255)
                                                                                                                      ccTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGccgacc                   >  7:236273/1‑134 (MQ=255)
                                                                                                                         tGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCCCACCCAGTTGCGCCTGGTTGCCGCCCGAgg               >  4:279632/1‑135 (MQ=255)
                                                                                                                           cAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCAGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCa            >  8:228684/1‑136 (MQ=255)
                                                                                                                            aaGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGGCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCa            <  3:168431/135‑1 (MQ=255)
                                                                                                                                gccgcgGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGGTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTa        <  3:360526/133‑1 (MQ=255)
                                                                                                                                 gcgcGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAgg      <  8:425014/136‑1 (MQ=255)
                                                                                                                                  cgcgGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAgg      <  5:291511/135‑1 (MQ=255)
                                                                                                                                  cgcgGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAgg      <  3:203172/135‑1 (MQ=255)
                                                                                                                                  cgcgAGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAgg      <  5:301791/135‑1 (MQ=255)
                                                                                                                                  cgcgAGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAgg      <  5:307613/135‑1 (MQ=255)
                                                                                                                                      ggCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAGGCCAc  <  1:188442/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
AGGCTGTCGTACAACGCATCGGAGCCGGACAACGAGCTGGTGTGGCTGAGCGCCAGTTCAGCGCCGATCTGCGAAACGCCCGTCTTCAATGCAATGATCGGCGTGCCCTGCTGCAGCGCCTTGCAAGCGGCGCGGGCAAAGCCCGGCACGTTCTTCAAGCCTTCCAGGTGCAGGCCGATGGCGGTTACCCGTGGGTCGTCGAGCAGCACATCCATCAGCTCGGCCACACCCAGTTGCGCCTGGTTGCCGACCGAGGCCATGTAGGCCAC  >  KT2440/4242872‑4243140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑